Method for extracting microalgae oil by adopting assistance of feeding digestive wall breaking of protozoon

A technology of protozoa and assisted extraction, which is applied in the direction of biofuel, fat production, fat oil/fat production, etc., can solve the problems of high cost and large water pollution, and achieve the effect of low pollution, low cost and high efficiency

Inactive Publication Date: 2018-03-13
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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Problems solved by technology

This scheme has the problems of large water pollution and high cost

Method used

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  • Method for extracting microalgae oil by adopting assistance of feeding digestive wall breaking of protozoon

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Embodiment 1

[0032] see figure 1 flow shown, to 1L the concentration is 1 x 10 8 3000 Daphnia were added to the chlorella algae liquid per mL, and the algae cells were broken through the feeding and digestion process of the Daphnia. The treatment time was 6 hours. / min centrifuge for 10min, use a 100-mesh filter to filter Daphnia, add 40mL of n-hexane / isopropanol with a volume ratio of 3:1 to the obtained algae mud, place it on a shaker for 3h, and add 10mL of 0.9% NaCl centrifugal layering, absorption of organic solvent and oil mixture, until the organic solvent is completely volatilized, after drying in a blast drying oven at 60°C, the crude oil can be obtained. The oil extraction rate can reach more than 98%, which is comparable to the traditional wall breaking method. Compared with that, the method provided by the invention has the advantages of high wall breaking efficiency and low cost.

Embodiment 2

[0034]see figure 1 flow shown, to 1L the concentration is 1 x 10 9 4000 brine shrimp were added to the Scenedesmus solution per mL, and the algae cells were broken through the feeding and digestion process of the brine shrimp. The treatment time was 12 hours. Centrifuge for 5 minutes, filter the brine shrimp with an 80-mesh filter, add 20 mL of n-hexane / isopropanol with a volume ratio of 2:1 to the obtained algae mud, place it on a shaker for 6 hours, and add 20 mL of 0.9% NaCl after the reaction is completed. Layering, absorption of organic solvent and oil mixture, after the organic solvent is completely volatilized, the crude oil can be obtained after drying in a blast drying oven at 80°C, and the oil extraction rate can reach more than 98%. Compared with the traditional wall breaking method, The method provided by the invention has the advantages of high wall breaking efficiency and low cost.

[0035] This scheme constructs a low-cost, high-efficiency, low-pollution techn...

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Abstract

The invention relates to a method for extracting microalgae oil by adopting the assistance of feeding digestive wall breaking of a protozoon. The method comprises the following steps: A: cell wall breaking: feeding a certain number of protozoa into an appropriate amount of algae solution, and performing the wall breaking treatment for the algae cells by utilizing the feeding digestion process of the protozoa; B: centrifugally filtering; C: extracting with a solvent; D: oil extracting, wherein the oil extraction rate can reach up to 98 percent or higher. The method for extracting the microalgaeoil by adopting the assistance of the feeding digestive wall breaking of the protozoon has the advantages of high efficiency, low cost, low pollution and industrialization; the wall breaking of waterflea digestion system is applied to the microalgae oil extraction process, so that the extraction cost of the microalgae oil can be effectively decreased, the extraction time is shortened, the harm of the extraction process on the environment and human body can be weakened and even eliminated, and the method can support the industrialization of the microalgae biological diesel.

Description

technical field [0001] The invention belongs to the field of microalgae biodiesel, in particular to a method for assisting extraction of oil from microalgae by using protozoa to ingest, digest and break walls. Background technique [0002] As a very important link in the microalgae biodiesel industry chain, oil extraction mainly adopts the combination of pretreatment and wall breaking and organic solvent extraction. Microalgal cells are small and have a relatively tough cell wall structure. The carbohydrate polymers in the algal cell wall are mainly composed of cellulose, xylose, glucose, fructose and mannose, and oil exists in the microalgal cell body. Therefore, pretreatment of algal cell wall breaking is a necessary condition for oil extraction. Microalgae cell wall breaking methods are mainly divided into physical methods, chemical methods and biological methods. Physical methods mainly include methods such as ultrasound, bead milling, microwave, pulsed electric field, ...

Claims

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Application Information

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IPC IPC(8): C11B1/02C11B1/04C11B1/10
CPCC11B1/025C11B1/04C11B1/10Y02E50/10
Inventor 金文标涂仁杰韩松芳丁婉晴
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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