Method for extracting pachymic acid and pachymaran from poria peel

A Poria polysaccharide and Poria bark technology, applied in the directions of steroids, organic chemistry, etc., can solve problems such as waste of Poria bark resources, and achieve the effects of saving traditional Chinese medicine resources, increasing added value, and reducing risks.

Inactive Publication Date: 2018-03-20
长沙爱扬医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Aiming at the resource waste problem of Poria cocos bark, in order to utilize Poria cocos bark more rationally, the present invention provides a kind of production technology that is simple to operate, process is reasonable, extracts pachymic acid and pachycosan at the same time rapidly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033]Crush Poria cocos skin, pass through a 100-mesh sieve, weigh 5 kg, add 50 L of 95% ethanol solution to reflux and extract 3 times, each time for 2 hours, filter, and combine the filtrates to obtain filtrate I and filter residue I. The filtrate I was concentrated under reduced pressure at 55°C to 65°C to obtain 5L of extract I. Add extract I to 10 L of ethyl acetate and extract twice for 1 hour each time, combine the ethyl acetate extracts, concentrate to dryness, and obtain 0.3 kg extract II. Add 3L of 80% ethanol to dissolve the extract II, put it on a silica gel chromatography column (200-300 mesh), absorb it for 30min, elute with 8L of 40% ethanol, discard the eluent, and then use 15L of 95% ethanol After elution, the eluate I was collected, concentrated until crystals were precipitated, and 48.2 g of crude crystals were obtained by filtration. Add 0.2L of 95% ethanol to dissolve the crude crystals, add 0.6L of water, refrigerate and crystallize to obtain 20.3g of pu...

Embodiment 2

[0035] Pulverize Poria cocos skin, pass through 80-mesh sieve, weigh 10kg, add 80L of 95% ethanol solution to reflux extraction for 3 times, each time for 2 hours, filter, and combine the filtrates to obtain filtrate I and filter residue I. The filtrate I was concentrated under reduced pressure at 55°C-65°C to obtain 8L of extract I. The extract I was extracted twice with 16 L of ethyl acetate, each time for 1 hour, the ethyl acetate extracts were combined, concentrated to dryness, and 0.7 kg of extract II was obtained. Dissolve the extract II in 6L of 90% ethanol, put it on a silica gel chromatography column (200-300 mesh), absorb for 30min, then elute with 10L of 50% ethanol, discard the eluent, and then use 30L of 95% ethanol After elution, the eluent I was collected, concentrated until crystals were precipitated, and filtered to obtain 0.1 kg of crude crystals. Add 0.4L of 95% ethanol to dissolve the crude crystals, add 1L of water, refrigerate and crystallize to obtain 4...

Embodiment 3

[0037] Crush Poria cocos skin, pass through a 120-mesh sieve, weigh 15 kg, add 120 L of 95% ethanol solution to reflux extraction for 3 times, each time for 2 hours, filter, and combine the filtrates to obtain filtrate I and filter residue I. The filtrate I was concentrated under reduced pressure at 55°C to 65°C to obtain 20L of extract I. Add extract I to 40 L of ethyl acetate and extract twice, each time for 1 hour, combine the ethyl acetate extracts, concentrate to dryness, and obtain 1.2 kg extract II. Dissolve the extract II in 10L of 80% ethanol, put it on a silica gel chromatography column (200-300 mesh), absorb for 30min, then elute with 30L of 70% ethanol, discard the eluent, and then use 45L of 95% ethanol After elution, the eluate I was collected, concentrated until crystals were precipitated, and filtered to obtain 0.16 kg of crude crystals. Add 0.8 L of 95% ethanol to dissolve the crude crystals, add 2 L of water, refrigerate and crystallize to obtain 65.8 g of p...

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Abstract

The invention relates to a method for extracting pachymic acid and pachymaran from poria peel, wherein the method comprises the following steps: performing alcohol reflux extraction on poria peel, filtering, and concentrating the filtrate to obtain filtrate and filter residue; concentrating the filtrate and adding ethyl acetate for extraction; concentrating the extract, purifying on a silica gel column, and crystallizing to obtain pachymic acid; adding a sodium hydroxide solution into the filter residue, and performing reflux extraction; filtering; adding acid into the filtrate to regulate thepH value; adding alcohol for precipitation; washing the precipitate and drying to obtain pachymaran. According to the method, active ingredients in the poria peel can be fully utilized, high-purity pachymic acid and pachymaran can be obtained at the same time, the utilization rate of the poria peel is greatly increased, the problem of resource waste is solved, and the production cost is lowered.

Description

Technical field [0001] The invention relates to a method for extracting pachymic acid and polysaccharide from poria cocos cortex, belonging to the technical field of natural product chemistry. Background technique [0002] Poria cocos bark is the dry outer skin of Poria cocos (Schw.) Wolf sclerotia of Polyporaceae fungus. More than July to September excavation, when processing "Poria slices" and "Poria pieces", collect the peeled skin and dry in the shade. Poria cortex is the peeled Poria cocos skin, with different shapes and sizes, flat nature, sweet and light taste, special function of water and swelling, mainly contains tetracyclic triterpene acids and polysaccharides, and triterpenoids have a wide range of pharmacological activities , such as diuretic, anti-hemolysis, anti-cancer, anti-inflammatory, anti-bacterial, anti-viral, lowering cholesterol, killing molluscs and anti-fertility. Poria cocos polysaccharide has the functions of protecting the liver, reducing alanin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J9/00C08B37/00
CPCC07J9/005C08B37/0003
Inventor 姚蓉
Owner 长沙爱扬医药科技有限公司
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