Atractylodes macrocephalaon polysaccharide extraction method

Abstract

The invention discloses an atractylodes macrocephalaon polysaccharide extraction method which comprises the following steps: (1) taking dry bighead atractylodes rhizome or / and stem powder, adding distilled water of 85 DEG C or more to be extracted to obtain leach liquor and adjusting a temperature of the leach liquor to 30 DEG C to 80 DEG C; (2) adding distilled water into the extraction liquid obtained in the step (1), meanwhile adding a sulfate solution, adjusting a pH value to 3 to 8, reacting for 0.5 to 3.0h and filtering to obtain first extracting solution and filter residues; (3) repeatedly extracting the filter residues obtained in the step (1) and the step (2) at least once to obtain multiple extraction solutions, combining the multiple extraction solutions, evaporating and concentrating, adding absolute ethyl alcohol, standing overnight and filtering to obtain precipitate, wherein the obtained precipitate is washed and dried to obtain atractylodes macrocephalaon polysaccharidecrude product chelated with metal ions. The extraction method disclosed by the invention has the advantages of low technological difficulty, more moderate extraction condition, higher yield and better polysaccharide biological activity retention.

Description

technical field [0001] The invention relates to a method for plant polysaccharides, in particular to a method for extracting Atractylodes macrocephala polysaccharides. Background technique [0002] At present, the extraction methods of Atractylodes macrocephala polysaccharides are mainly hot water extraction method, enzymatic method, microwave-assisted extraction method, supercritical extraction method and ultrasonic-assisted extraction method. The most widely used extraction method is hot water extraction. [0003] Yuan Haimei [1] etc. using the orthogonal test optimization design, water extraction and alcohol precipitation method to extract Atractylodes macrocephala crude polysaccharide. [0004] The general synthesis method of polysaccharide-iron complexes is to make refined polysaccharides into a certain concentration of aqueous solution, then add trisodium citrate, mix well and add ferric chloride to control the pH value to about 8.0, and filter the supernatant alcoho...

AI Technical Summary

Problems solved by technology

[0007] In the prior art, metal polysaccharide complexes are usually synthesized by leaching from raw materials to obtain refined polysaccharides, and then chelate with specific metal ions under suitable conditions to obtain polysaccharide metal complexes; the refined polysaccharides are generally separated by hot water leaching Obtained by purification; hot water extraction method usually requires high extraction temperature and many extraction times, so the energy loss in production is large, and the preparation process of the complex is complicated, which also increases the energy loss in production

[0008] Although there have been some studies on the synthesis of polysaccharide metal complexes, there is no report in the literature on the synthesis and preparation process of Atractylodes macrocephala polysaccharide metal complexes.