Supercharge Your Innovation With Domain-Expert AI Agents!

Saccharomyces cerevisiae transformation method for batch transformation, and application thereof

A Saccharomyces cerevisiae, batch technology, applied in the fields of biochemistry and molecular biology, can solve the problems of long time period, achieve the effect of simple operation and shortened test time

Inactive Publication Date: 2018-03-23
TOBACCO RES INST CHIN AGRI SCI ACAD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most of the currently existing transformation methods for Saccharomyces cerevisiae need to determine the concentration of yeast growth (i.e. OD 600 value), and need different temperature water bath heat shock treatment, repeated washing and long time period and other disadvantages

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Saccharomyces cerevisiae transformation method for batch transformation, and application thereof
  • Saccharomyces cerevisiae transformation method for batch transformation, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The genome sequencing of the diploid wild species of peanut has been completed. The HSP90-RAR1-SGT1 complex is widely involved in plant disease resistance response and growth and development. After analysis, the whole peanut genome includes at least 5 HSP90 , 1 RAR1 , 3 SGT1 Gene. In order to verify the interaction relationship between the members of the HSP90-RAR1-SGT1 complex in peanut, the present invention HSP90 , RAR1 and SGT1 Gene self-stimulation activity as well as pairwise interactions were detected. This takes about 80 conversion events. But utilizing the technical scheme of the present invention can be easily completed at one time. The specific technical scheme is as follows:

[0024] 1. In the afternoon of the first day, take a fresh Saccharomyces cerevisiae colony growing about 2-3 mm on YPDA solid medium, and inoculate it in more than 160 mL of fresh YPDA liquid medium under sterile conditions, at 30°C and 200 rpm Cultivate for 12-24 h.

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Saccharomyces cerevisiae transformation method for batch transformation, and an application thereof. The method comprises the following four steps: 1) taking about 2-3 mm fresh Saccharomyces cerevisiae monoclone which grows on a YPDA solid medium, inoculating a fresh YPDA liquid medium with the fresh Saccharomyces cerevisiae monoclone under an aseptic condition, and performing culture for 12-16 h; 2) collect thalli, removing the obtained supernatant, and adding an LPS yeast transformation solution; 3) adding a plasmid to be transformed, and performing full vortex shaking; and 4) standing the obtained solution at room temperature for a period of time, collecting the thalli, removing the obtained supernatant, dissolving the thalli in a sterile sorbitol solution, coating a proper yeast selection medium with the obtained solution by using a ball, inverting the coated yeast selection medium in an incubator, and growing a normal colony in 2-3 days. The method has the advantages of simplicity and fastness in operation, great shortening of the test time, and realization of large-batch conversion, is a simple and rapid yeast transformation method in a laboratory, and can be applied to yeast induced expression and protein interaction verification.

Description

technical field [0001] The invention belongs to the fields of biochemistry and molecular biology, and in particular relates to a transformation method of Saccharomyces cerevisiae for batch transformation and its application. Background technique [0002] In the study of the function of a protein encoded by a gene, it is sometimes necessary to induce its expression in Saccharomyces cerevisiae or to verify its interaction with other proteins, which requires first transforming the yeast expression vector carrying the gene into Saccharomyces cerevisiae. Most of the currently existing transformation methods for Saccharomyces cerevisiae need to determine the concentration of yeast growth (i.e. OD 600 Value), and need different temperature water bath heat shock treatment, repeated washing and long time period and other disadvantages. Contents of the invention [0003] The object of the present invention is to provide a method for transforming Saccharomyces cerevisiae used for ba...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/81C12R1/865
CPCC12N15/81
Inventor 苑翠玲孔英珍丁安明
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com