Detection chip and detection device based on micro-fluidic technology

Abstract

The invention relates to the technical field of micro-fluidic chips, and provides an electrochemical luminescence detection chip and detection device based on a micro-fluidic technology. The detectionchip comprises a base layer, a loading layer and a sealing covering layer; a plurality of electrode units are arranged on the base layer; a plurality of through holes are formed in the loading layer;the plurality of through holes and the plurality of electrode units are in one-to-one correspondence to form a plurality of reaction tanks; a plurality of grooves are formed in the bottom of the sealing covering layer; the plurality of grooves are in one-to-one correspondence to the tops of all reaction tanks to form top covers of the reaction tanks; the sealing covering layer is also provided with an inflow hole and an outflow hole; the plurality of grooves communicate with each other through a micro-fluidic channel; and two ends of the micro-fluidic channel respectively communicate with theinflow hole and the outflow hole. The detection chip provided by the invention comprises a plurality of reaction tanks, and can be used to carry out detection of multiple biochemical or chemical indexes on a sample at the same time. In a using process, only the outflow hole and the inflow hole communicate with outside, and most of reactions are completed in a closed micro-fluidic channel system,so that the interference of the external environment to the reactions and detection process is effectively reduced.

Description

technical field [0001] The invention relates to the technical field of microfluidic chips, and in particular provides an electrochemiluminescent detection chip based on microfluidic technology. Background technique [0002] Electrochemiluminescence (ECL) is a combination of chemiluminescence and electrochemistry. By applying a certain voltage to perform an electrochemical reaction, some electrogenerated substances are generated on the electrode surface, and then these electrogenetic substances or between the electrobiological substances and the system Some components form an excited state through electron transfer, and a luminescent phenomenon occurs when the excited state returns to the ground state. [0003] Electrochemiluminescence has the advantages of high sensitivity, stable reaction system, and fast analysis speed, and has been widely used in the fields of biology, medicine, pharmacy, clinical, environment, food, immune and nucleic acid hybridization analysis and indu...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an electrochemiluminescence detection chip and detection equipment based on microfluidic technology, aiming to solve the problem of using electrochemiluminescence technology for detection in the prior art with many operating steps, complex systems, and susceptibility to environmental interference. , high electrode requirements, repeated use and easy cross-contamination and other technical problems

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