A set of SNP loci for canine strain identification

Abstract

The invention discloses a set of SNP (Single Nucleotide Polymorphism) sites for canine strain identification. The SNP sites for canine strain identification comprise SNP sites given in a table 2. Theinvention further discloses a chip used for canine strain identification. The chip is used for measuring alleles of the SNP sites given in the table 2.

Description

technical field [0001] The invention belongs to the field of gene detection, and more specifically, the invention relates to a set of SNP loci for identification of canine strains. Background technique [0002] The methods of canine genetic strain identification are mainly divided into three categories. The first category: judge the breed of dogs based on the recorded dog pedigree information. The second category: the breed is judged by the appearance of dogs. The third category: judging strains by DNA microsatellite loci (STR). [0003] The technical shortcomings of the above three types of products are as follows: The first type: 1) It is necessary for dogs to maintain a systematic genealogy file. If the file is lost or tampered with (bad pet dealers often use this method to deceive consumers), the pet breed Judgment will be wrong. 2) Most of the mixed breed pet dogs circulating in the market or kept by pet owners do not have systematic genealogical documents, so it is...

AI Technical Summary

Problems solved by technology

[0003] The technical shortcomings of the above three types of products are as follows: The first type: 1) Dogs need to keep a systematic genealogy file. If the file is lost or tampered with (bad pet dealers often use this method to deceive consumers), the pet breed Judgment will be wrong

2) Most of the mixed breed pet dogs circulating in the market or raised by pet owners do not have systematic genealogical documents, so it is impossible to accurately judge the composition of their breeds

The second category: 1) Through the appearance of pets, it is difficult to distinguish dog breeds with similar strains

2) The judgment is very subjective and cannot be supported by objective evidence

3) It is impossible to quantitatively determine how high the purity of the strain is

4) If it is a mixed breed, several books cannot accurately judge the composition of the strain from the appearance

2) The experimental process is difficult to be easily compatible with the next-generation sequencing platform, and it cannot be effectively combined with other next-generation sequencing DNA-based disease detection and trait prediction into an integrated experimental method

3) Due to the small number of STR marker sites and the limited amount of information, this method has acceptable resolution for purebred bloodlines, but cannot make accurate judgments for dogs with mixed breeds

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