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Method for rapidly extracting cereal protein

An extraction method and protein technology are applied in the field of grain protein extraction, which can solve the problems of low extraction rate, low economic benefit, long extraction cycle and the like, and achieve the effects of shortening extraction time, improving extraction rate and shortening operation time.

Inactive Publication Date: 2018-04-06
安徽省东博米业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Protein is an essential biomacromolecule in living organisms. It is the macromolecular substance with the most abundant content and the most functions in cells. It plays an important role in various life activities and is the material basis for sustaining life. According to the Food and Agriculture Organization of the United Nations, adult People should consume more than 75g of protein per day, while the world average level is only 68.8g, and the current average level in my country is only 60g. The lack of protein intake is mainly due to the insufficient absolute intake of protein and the imbalance of amino acids in the protein intake. Currently, The primary way to solve the problem of insufficient protein intake is to develop new protein sources. The nitrogen conversion route requires more economic costs and a longer time period than the extraction of plant proteins, and the utilization cost of plant proteins is relatively low. Therefore, processing and utilization Vegetable protein is currently the main measure to solve the shortage of protein supply in our country. Among them, the extraction of grain protein has great significance, but there are few studies on the extraction of grain protein in the prior art. Separation and other methods for protein extraction, the extraction cycle takes a long time, the extraction rate is low, and the economic benefit is low, so it cannot be popularized and used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A method for rapidly extracting grain protein, comprising the following steps:

[0015] (1) Dehulling the grains, irradiating the obtained grains with far-infrared rays with a wavelength of 7 μm for 2 minutes, and then grinding them to a particle size of 100 mesh to obtain grinding materials for later use;

[0016] (2) Take 100mg of the obtained grinding material and add it to 2ml of acetone solution at a temperature of 45°C, extract it under the action of ultrasonic waves with a frequency of 64kHz and a power of 80-100kW for 10 minutes, and then centrifuge at a centrifugal force of 8000g for 10 minutes to obtain preliminary extraction liquid;

[0017] (3) Remove the acetone solution in the preliminary extract, and then add saline solution with a mass concentration of 4% equivalent to twice its weight. The temperature of the saline solution is 50°C. After keeping warm for 4 minutes, centrifuge with a centrifugal force of 12000g After 7 minutes, the resulting supernatan...

Embodiment 2

[0021] A method for rapidly extracting grain protein, comprising the following steps:

[0022] (1) Dehulling the grains, irradiating the obtained grains with far-infrared rays with a wavelength of 6 μm for 2 minutes, and then grinding them to a particle size of 80 mesh to obtain grinding materials for later use;

[0023] (2) Take 100mg of the obtained grinding material and add it to 2ml of acetone solution at a temperature of 42°C, extract it under the action of ultrasonic waves with a frequency of 60kHz and a power of 80kW for 12 minutes, and then centrifuge at a centrifugal force of 8000g for 10 minutes to obtain a preliminary extract;

[0024] (3) Remove the acetone solution in the preliminary extract, and then add saline solution with a mass concentration of 5% equivalent to 2.5 times its weight. The temperature of the saline solution is 55°C. After keeping it warm for 3 minutes, centrifuge it with a centrifugal force of 12000g After 6 minutes, the resulting supernatant wa...

Embodiment 3

[0028] A method for rapidly extracting grain protein, comprising the following steps:

[0029] (1) Dehulling the grains, irradiating the obtained grains with far-infrared rays with a wavelength of 8 μm for 2 minutes, and then grinding them to a particle size of 80-100 mesh to obtain abrasives for use;

[0030] (2) Take 100mg of the obtained grinding material and add it to 2ml of acetone solution at a temperature of 48°C, extract it under the action of ultrasonic waves with a frequency of 60kHz and a power of 100kW for 12 minutes, and then centrifuge at a centrifugal force of 8000g for 10 minutes to obtain a preliminary extract;

[0031] (3) Remove the acetone solution in the preliminary extract, and then add 1.5 times its weight of saline with a mass concentration of 5%. The temperature of the saline is 45°C. After keeping warm for 3 minutes, centrifuge with a centrifugal force of 12000g After 6 minutes, the resulting supernatant was dialyzed and dried to obtain protein.

[0...

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Abstract

The invention belongs to the technical field of cereal protein extraction, in particular to a method for rapidly extracting cereal protein. The method for rapidly extracting the cereal protein comprises preparation of an abrasive, preparation of preliminary extraction liquid and secondary centrifugation to obtain the protein. Compared with the prior art, the method has the advantages that far-infrared irradiation on cereal grains is beneficial to the crushing of the cereal grains and also can further increase the extraction rate of the preliminary extraction liquid and shorten the extraction time, and the protein extraction rate can be increased to 60% or above through a combination of secondary extraction. Compared with the traditional rice protein extraction time of 4 to 8 hours, by means of the method, the entire extraction process can be completed in only about 1 hour, and the operation time can be shortened on the basis of ensuring the extraction rate.

Description

technical field [0001] The invention belongs to the technical field of grain protein extraction, and in particular relates to a rapid extraction method of grain protein. Background technique [0002] Protein is an essential biomacromolecule in living organisms. It is the macromolecular substance with the most abundant content and the most functions in cells. It plays an important role in various life activities and is the material basis for sustaining life. According to the Food and Agriculture Organization of the United Nations, adult People should consume more than 75g of protein per day, while the world average level is only 68.8g, and the current average level in my country is only 60g. The lack of protein intake is mainly due to the insufficient absolute intake of protein and the imbalance of amino acids in the protein intake. Currently, The primary way to solve the problem of insufficient protein intake is to develop new protein sources. The nitrogen conversion route req...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/34C07K1/14
Inventor 刘德田
Owner 安徽省东博米业有限公司