Drug-metabolizing enzyme metabotype assessment method

Abstract

The invention provides a drug metabolic enzyme metabolism type evaluation method, comprising steps: S1, acquiring haploid variant allele names of genes from a preset gene data bank, to form a gene information table; S2, according to the gene information table, obtaining a diploid variant allele name sequence; S3, forming metabolism types, to obtain a metabolism type information table; S4, according to gene detection results, obtaining a locus diploid name table; S5, obtaining a first haploid variant allele name sequence; S6, according to enzymatic activity of each haploid variant allele, obtaining a second haploid variant allele name sequence; S7, from the second haploid variant allele name sequence, obtaining a diploid variant allele name result; S8, retrieving from the metabolism type information table, to obtain a metabolism type detection result. The drug metabolic enzyme metabolism type evaluation method can accurately determine a gene metabolism type, and has important meaning on drug metabolic enzyme gene detection and personalized medication aspects.

Description

technical field [0001] The invention relates to the technical field of gene detection, in particular to a method for evaluating the metabolic type of drug-metabolizing enzymes. Background technique [0002] In pharmacogenetics, the most widely used nomenclature to describe pharmacogenetic genotypes differs from other genetic tests such as the cytochrome p450 family of genes involved in metabolism, Human Cytochrome P450 (CYP) Allelic Nomenclature Committee (http: / / www.cypalleles.ki.se) is recommended to be named with "*", see figure 1 . In this system, the most common allele is designated as "*1". Essentially, a "*" allele is the result of a combination of mutations at multiple base positions. At present, whether it is gene sequencing technology or SNP (single nucleotide polymorphism) chip detection, only independent genotyping (genotype) judgments can be performed on multiple loci, and the SNP loci that appear cannot be specifically judged. It is cis, that is, on the sa...

AI Technical Summary

Problems solved by technology

At present, whether it is gene sequencing technology or SNP (single nucleotide polymorphism) chip detection, only independent genotyping (genotype) judgments can be performed on multiple loci, and the SNP loci that appear cannot be specifically judged. Are in cis, that is, on the same chromosome; or in trans, that is, appear on different chromosomes

Therefore, it is also not possible to assign "*" alleles to the detected genes

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