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Pre-processing method for strengthening corncob and corncob residue efficient enzymatic hydrolysis

A corn cob and enzymatic hydrolysis technology, applied in biofuels, fermentation, etc., can solve problems such as patent publications that have not been found, and achieve the effects of improving enzymatic hydrolysis efficiency, alleviating the demand for fossil energy, and reducing input costs

Inactive Publication Date: 2018-04-10
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through the search, no patent publications related to the patent application of the present invention have been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A pretreatment method for strengthening the high-efficiency enzymatic hydrolysis of corncobs and corncob residues, the steps are as follows:

[0027] [1] Slurry preparation: Air-dry and pulverize the corn cob raw material, screen it with a 40-mesh sieve, and put the obtained raw material into a sealed bag for subsequent use;

[0028] [2] Mechanical pretreatment: Weigh a certain absolute dry mass of corncob raw material treated as above, add water to dilute and prepare a raw material suspension with a mass concentration of 4.0%, put it into a grinder, and when the rotating speed is 1000rpm, the raw material is processed. Treat for 5 minutes, then collect the ground corncobs, filter and dehydrate to achieve the purpose of concentration, and store them at low temperature (2-10°C) for subsequent use;

[0029] [3] Enzymatic hydrolysis: Weigh a certain absolute dry mass of corncobs treated as above and put them into the reaction kettle as the substrate for enzymatic hydrolysi...

Embodiment 2

[0036] A pretreatment method for strengthening the high-efficiency enzymatic hydrolysis of corncobs and corncob residues, the steps are as follows:

[0037] [1] Slurry preparation: Air-dry and pulverize the corn cob raw material, screen it with a 40-mesh sieve, and put the obtained raw material into a sealed bag for subsequent use;

[0038] [2] Mechanical pretreatment: Weigh a certain absolute dry mass of the corncob raw material treated above, add water to dilute and prepare a raw material suspension with a mass concentration of 4.0%, put it in a grinder, and grind the raw material at a speed of 1000rpm After 10 minutes, the ground corncobs were collected, dehydrated by suction to achieve the purpose of concentration, and stored under low temperature conditions (2-10°C) for subsequent use;

[0039] [3] Enzymatic hydrolysis: Weigh a certain absolute dry mass of corncobs treated as above and put them into the reaction kettle as the substrate for enzymatic hydrolysis, add HAc-NaAc...

Embodiment 3

[0046] A pretreatment method for strengthening the high-efficiency enzymatic hydrolysis of corncobs and corncob residues, the steps are as follows:

[0047] [1] Slurry preparation: air-dry and pulverize the raw material of corn cob residue, screen it with a 40-mesh sieve, and put the obtained raw material into a sealed bag for subsequent use;

[0048] [2] Mechanical pretreatment: Weigh a certain absolute dry mass of the corn cob residue raw material treated above, add water to dilute and prepare a raw material suspension with a mass concentration of 4.0%, place it in a grinder, and carry out the raw material under the speed of 2000rpm Grind for 30 minutes, then collect the ground corncob residue, filter and dehydrate to achieve the purpose of concentration, and store it at low temperature (2-10)°C for subsequent use;

[0049] [3] Enzymatic hydrolysis: Weigh a certain absolute dry weight of the corncob residue treated above as the substrate for enzymatic hydrolysis and put it i...

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Abstract

The invention discloses a pre-processing method for strengthening corncob and corncob residue efficient enzymatic hydrolysis. The pre-processing method comprises the following steps: (1) preparing slurry; (2) conducting mechanical pre-processing; and (3) conducting enzymatic hydrolysis, so as to obtain a hydrolysate, so that the corncob and corncob residue efficient enzymatic hydrolysis is strengthened. The pre-processing method provided by the invention, by processing corncobs and corncob residues by virtue of a mechanical pre-processing way, can reduce physical dimensions of raw materials, reduce a degree of crystallinity and improve the specific surface area and swelling performance of the raw materials, and subsequently, the efficiency of enzymatic hydrolysis is improved, and a relatively high glucose concentration and relatively high glucose conversion rate are finally guaranteed; with the application of the method, high-value utilization of the corncobs and the corncob residues can be achieved, and meanwhile, input cost of the enzymatic hydrolysis is reduced, the efficiency of the enzymatic hydrolysis is improved and a raw material foundation is provided for subsequent processing and utilization or for production of high-value-added products; therefore, the application field and scope of the corncobs and the corncob residues are expanded.

Description

technical field [0001] The invention belongs to the technical field of combining high-efficiency utilization of biomass resources and agricultural waste, and relates to a method for realizing high-efficiency enzymatic hydrolysis by adopting mechanical pretreatment, especially a pretreatment method for strengthening high-efficiency enzymatic hydrolysis of corncobs and corncob residues . technical background [0002] Biomass enzymatic hydrolysis is a new type of green energy utilization method, that is, by enzymatically hydrolyzing lignocellulosic raw materials and applying fermentable sugars to fermentation processes, organic chemical raw materials such as butanol, acetone and ethanol and biomass can be produced Fuel, which can also be used to produce feed, food and pharmaceuticals, provides a demonstration of the biomass refining process. In most cases, the raw material for enzymatic hydrolysis is screened wood fiber. The input cost of enzymatic hydrolysis is high, which is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14C12P7/10
CPCC12P7/10C12P19/14C12P2201/00Y02E50/10
Inventor 刘苇汪兵侯庆喜王思
Owner TIANJIN UNIV OF SCI & TECH
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