Weissella confusa 3DW having high antagonism effect on Edwardsiella tarda

A technology of Edwardsiella tarda and Weissella, which is applied in the direction of bacteria, antibacterial drugs, and methods based on microorganisms, can solve problems such as reducing water bodies, and achieve high antagonistic effects

Inactive Publication Date: 2018-04-13
DALIAN OCEAN UNIV
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  • Abstract
  • Description
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Problems solved by technology

However, so far there is no relevant report on strains that can significantly reduce the pH of water and have a high antagonistic effect on Edwardsiella tarda

Method used

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  • Weissella confusa 3DW having high antagonism effect on Edwardsiella tarda
  • Weissella confusa 3DW having high antagonism effect on Edwardsiella tarda
  • Weissella confusa 3DW having high antagonism effect on Edwardsiella tarda

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Experimental program
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Embodiment Construction

[0013] 1. Isolation and purification of strains:

[0014] Taking the saline-alkali pond water environment in Panjin area as the source of bacteria isolation, the samples were processed according to the method of ten-fold gradient dilution, and the water samples with appropriate dilution were selected and spread on the MC solid medium with a salinity of 10. The selected MC medium had The colony of the calcium-dissolving circle was purified and cultivated to obtain a single pure colony, which was streaked on the MC solid medium and cultured at a constant temperature of 28 ℃ for 24-36 h to obtain an acid-producing bacterium, which was named 3DW.

[0015] 2. Identification of Strain 3DW

[0016] For the strain 3DW Gram stain results as figure 1 shown.

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Abstract

The invention discloses a Weissella confusa 3DW having a high antagonism effect on Edwardsiella tarda. The Weissella confusa 3DW is preserved in China General Microbiological Culture Collection Centerwith the preservation codename being CGMCC 14656. A difference between the inhibition zone diameter and the colony diameter of the Weissella confusa 3DW on the Edwardsiella tarda is 8.17 mm, so the Weissella confusa 3DW can effectively prevent and treat various infected diseases of aquatic products, caused by the Edwardsiella tarda. The strain can significantly reduce the pH value of water, can be applied to a micro-ecologic preparation to reduce the pH value of water in a saline and alkaline land culture pond, and is suitable for saline and alkaline land pond culture.

Description

technical field [0001] The invention relates to a fused Weissella strain, in particular to a fused Weissella 3DW which can significantly reduce the pH of water and has a high antagonistic effect on Edwardsiella tarda. Background technique [0002] Edwardsiella tarda Edwardsiella tarda Belonging to Gram-negative pathogenic bacteria, it can infect a variety of animals including fish, amphibians, reptiles and mammals. The general clinical symptoms of fish infection are exophthalmos and opacity, skin necrosis and muscle ulceration, and anal protrusion , Muscle and visceral organs produce abscesses and other phenomena, which have the characteristics of wide epidemic area, high morbidity and mortality. At present, the most common way to prevent and treat bacterial diseases is to use antibiotics, which can not only cause fish flora imbalance, increase of drug-resistant strains, and decline of immune function, but also cause harmful substances such as drugs to remain in fish produc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61P31/04C12R1/01
CPCC12N1/205C12R2001/01
Inventor 叶仕根杜明洋谢明旭李强黎睿君
Owner DALIAN OCEAN UNIV
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