Weissella confusa 3DW having high antagonism effect on Edwardsiella tarda
A technology of Edwardsiella tarda and Weissella, which is applied in the direction of bacteria, antibacterial drugs, and methods based on microorganisms, can solve problems such as reducing water bodies, and achieve high antagonistic effects
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[0013] 1. Isolation and purification of strains:
[0014] Taking the saline-alkali pond water environment in Panjin area as the source of bacteria isolation, the samples were processed according to the method of ten-fold gradient dilution, and the water samples with appropriate dilution were selected and spread on the MC solid medium with a salinity of 10. The selected MC medium had The colony of the calcium-dissolving circle was purified and cultivated to obtain a single pure colony, which was streaked on the MC solid medium and cultured at a constant temperature of 28 ℃ for 24-36 h to obtain an acid-producing bacterium, which was named 3DW.
[0015] 2. Identification of Strain 3DW
[0016] For the strain 3DW Gram stain results as figure 1 shown.
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