Primer set for detecting plasma miR-140-3p of child patient with biliary atresia and detection kit containing same
A detection kit and biliary atresia technology, which are applied in recombinant DNA technology, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of good specificity, high cost, unfavorable promotion and application, etc. The effect of high sensitivity and low price cost
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[0028] The present invention will be described in further detail below in conjunction with specific embodiments and accompanying drawings. This implementation case is carried out on the premise of the technology of the present invention, and the detailed implementation mode and specific operation process are given now, but the protection scope of the present invention is not limited to the following examples.
[0029] 1. Implement high-throughput sequencing of samples
[0030] 1. Grouping of sequencing samples The "mixed pool" method was used to group and mix samples. The collected 9 cases of BA patients were divided into three groups (A1-3) according to age and gender (3 persons / group), and 9 normal control children were divided into three groups according to age and sex. After age and sex were matched with the case group, they were also divided into three groups, N1~3; the equal volumes (200ul:200ul:200ul) of plasma samples from 3 cases in the group were mixed into 1 sample,...
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