Zymoid test paper used for detecting hydrogen peroxide and preparation method and application thereof
A technology of hydrogen peroxide and test paper, which can be applied to measurement devices, analysis by chemical reaction of materials, and material analysis by observing the impact on chemical indicators, which can solve the problems of interference color development, long preparation or measurement time, and test methods Cumbersome and other problems to achieve the effect of reducing usage and improving accuracy
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Embodiment 1
[0038] Detection of hydrogen peroxide (400mg / L) in aqueous solution: Cut the qualitative filter paper into strips with a width of 6mm and a length of 80mm. Take 10mg iron-based MIL-88NH 2 Disperse the enzyme-like catalyst into 10 mL of acetone, disperse ultrasonically, and continue to configure a 0.2 mg / mL 3,3',5,5'-tetramethylbenzidine in propanol solution as the developer solution. Take 50 μL of the catalyst dispersion and apply it on the cut test paper, dry it in a vacuum at 50°C, evenly spread 50 μL of the developer solution on the dry test paper, and dry it under vacuum at 50°C to obtain the prepared enzyme-like test paper. The loading amount of the obtained enzyme-like test paper iron-based MIL series metal organic framework material is 0.05 mg, and the loading amount of the color developer is 0.01 mg.
[0039] In order to detect different concentrations of hydrogen peroxide solution, first configure 100mg / L, 200mg / L, 400mg / L, 600mg / L, 800mg / L, 1000mg / L gradient concentrati...
Embodiment 2
[0041] Detection of hydrogen peroxide (20mg / mL) in aqueous solution: Cut the qualitative filter paper into strips with a width of 6mm and a length of 80mm. Disperse 30 mg of iron-based MIL-100 enzyme catalyst into 10 mL of acetone, disperse ultrasonically, and continue to configure a 2 mg / mL 3,3',5,5'-tetramethylbenzidine dimethyl sulfoxide solution as a display Toner solution. Take 50μL of catalyst dispersion and smear it on the cut test paper, dry it under vacuum at 40℃, evenly smear 50μL of the developer solution on the dry test paper, and dry it under vacuum at 40℃ to obtain the prepared enzyme-like test paper. The loading amount of the obtained enzyme-like test paper iron-based MIL series metal organic framework material is 0.15mg, and the loading amount of the color developer is 0.1mg.
[0042] In order to detect different concentrations of hydrogen peroxide solution, first configure 1mg / L, 5mg / L, 10mg / L, 30mg / L, 50mg / L, 100mg / L gradient concentration of hydrogen peroxide ...
Embodiment 3
[0044] Detection of hydrogen peroxide (4mg / L) in aqueous solution: Cut the qualitative filter paper into strips with a width of 6mm and a length of 80mm. Disperse 30 mg of iron-based MIL-101 type enzyme catalyst into 10 mL of acetone, disperse ultrasonically, and continue to configure a methanol solution of o-phenylenediamine with a concentration of 10 mg / mL as the developer solution. Take 50μL of catalyst dispersion and smear it on the cut test paper, dry it under vacuum at 40℃, evenly smear 50μL of the developer solution on the dry test paper, and dry it under vacuum at 40℃ to obtain the prepared enzyme-like test paper. The loading amount of the obtained enzyme-like test paper iron-based MIL series metal organic framework material is 0.15mg, and the loading amount of the color developer is 0.5mg.
[0045] In order to detect different concentrations of hydrogen peroxide solution, first configure 0.5mg / L, 2mg / L, 5mg / L, 10mg / L, 25mg / L gradient concentration of hydrogen peroxide so...
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