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Strain applicable to industrial cultivation of white Flammulina velutipes and application thereof

A technology for white Flammulina velutipes and Flammulina velutipes, which is applied in cultivation, application, plant cultivation and other directions, can solve the problems of easy variation, poor performance, and influence on production stability, and achieves the effects of high uniformity, white roots, and difficulty in opening umbrellas.

Inactive Publication Date: 2018-04-20
江苏华绿生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Strains are the key and basis for the industrial cultivation of Flammulina velutipes. The quality and stability of the strains ultimately determine whether high and stable yields can be achieved. However, the current domestically used Flammulina velutipes varieties generally perform poorly and are prone to variation, which has serious impact on the yield and stability of production. had a great impact

Method used

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  • Strain applicable to industrial cultivation of white Flammulina velutipes and application thereof
  • Strain applicable to industrial cultivation of white Flammulina velutipes and application thereof
  • Strain applicable to industrial cultivation of white Flammulina velutipes and application thereof

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Effect test

Embodiment 1

[0031] Breeding Method of Flammulina velutipes Strain CG-FVDA12

[0032] Flammulina velutipes strain CG-FVDA001 produces mushrooms under factory conditions, collects spores, dilutes the spores with sterile water to form a spore suspension, spreads it evenly on PDA solid medium, cultures at 18-20°C, and the spores germinate After the formation of monokaryotic hyphae, separate and purify the monokaryotic hyphae under a microscope, and obtain a pure culture of monokaryotic hyphae after culturing at 18-20°C, and perform a pairwise hybridization test on the obtained multi-strain monokaryotic mycelium After the two monokaryotic hyphae come into contact, place them for a period of time, pick the contact parts of the two, and examine them under a microscope. If there is a lock-like joint, the pairing is successful. Pick the culture block and inoculate it on a 90mm petri dish containing PDA solids. When the diameter of the filament is 3cm-4cm, the tip of a single dikaryotic hyphae is p...

Embodiment 2

[0043] The factory cultivation test of Flammulina velutipes (Flammulina velutipes) bacterial strain CG-FVDA12, concrete steps are as follows:

[0044] 1. Preparation of liquid strains

[0045] 1. Take the screened Flammulina velutipes strain CG-FVDA12 as the strain, inoculate it into the PDA test tube culture medium, and cultivate it at 18°C ​​for 8 days to expand the culture;

[0046] 2. Put it into the liquid Erlenmeyer flask culture medium, the formula is 20g first-grade white granulated sugar + 3g 200 mesh soybean meal + 0.6g magnesium sulfate + 0.6g potassium dihydrogen phosphate + 1L purified water, pH 6.0, shake culture at 18°C ​​for 9 days;

[0047] 3. The strains of the shake flask are inserted into the culture medium of the fermenter, and the formula is 12Kg first-grade white granulated sugar + 1.5Kg 200 mesh soybean meal + 300g magnesium sulfate + 300g potassium dihydrogen phosphate + 30mL defoamer + 700L pure water, pH6.0, After culturing at 18°C ​​for 9 days, it ...

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Abstract

The invention provides a strain applicable to industrial cultivation of white Flammulina velutipes. The strain is classified and named as Flammulina velutipes CG-FVDA12 and preserved in China GeneralMicrobiological Culture Collection Center with an accession number of CGMCC No. 12971. The invention also provides a method for industrial cultivation of white Flammulina velutipes by utilizing the Flammulina velutipes strain CG-FVDA12. The Flammulina velutipes strain CG-FVDA12 provided by the invention has the following advantages under the condition of industrial cultivation: after fruiting, sporocarps have white mushroom body, high uniformity, circular and complete caps, insusceptibility to cap-opening, white roots after root cutting, etc.; and extensive popularization and application can be realized.

Description

technical field [0001] The invention belongs to the technical field of industrial cultivation of edible fungi, and in particular relates to a strain suitable for industrial cultivation of Flammulina velutipes and an application thereof. Background technique [0002] Flammulina velutipes is a low-temperature fruiting edible fungus that occurs in late autumn to early spring. It is clump-shaped when wild. Flammulina velutipes has the characteristics of crisp and tender stipe, sticky and slippery cap, and delicious taste. It is rich in amino acids, and is also known as "enhancing wisdom mushroom "There are two strains of Flammulina velutipes, yellow and white. Compared with the yellow strain, the white strain has a better product appearance, so it is popular in the market. Most of the white strains sold in the market are products cultivated in factory mode. Compared with traditional cultivation, the factory cultivation mode uses various automation equipment to artificially creat...

Claims

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Application Information

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IPC IPC(8): A01G18/00
Inventor 余养朝林瑞虾张光忠邱昌里
Owner 江苏华绿生物科技股份有限公司
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