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Paenibacillus polymyxa for preventing and treating American ginseng root decay diseases and application thereof

A polymyxa spore-like and American ginseng root technology is applied in the field of polymyxa spore-like spore, which can solve the problems of species and scope limitation, economic loss of growers, lack of special drugs, etc., and achieves the effect of broad application prospect.

Active Publication Date: 2018-04-20
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the development of the concept of green agriculture, the country has stricter limits on pesticide residues on crops. The types and scope of pesticides used in the management of American ginseng are greatly restricted. The most effective pesticides against Fusarium containing pentachloronitrobenzene have been banned. , there is no special medicine when root rot occurs, which brings great economic losses to the growers

Method used

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  • Paenibacillus polymyxa for preventing and treating American ginseng root decay diseases and application thereof
  • Paenibacillus polymyxa for preventing and treating American ginseng root decay diseases and application thereof
  • Paenibacillus polymyxa for preventing and treating American ginseng root decay diseases and application thereof

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Effect test

Embodiment 1

[0024] Example 1, Screening of Paenibacillus polymyxa DN67

[0025] On July 2, 2016, samples were collected from the surface sediments of the offshore seabed in Yantai City, Shandong Province, and the microorganisms were separated and purified by the dilution coating method. 10g of mud samples were weighed and added to a triangular flask filled with 90mL of sterile water, and oscillated at 180r / min 30min, the resulting suspension was diluted to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Different dilutions were spread on the LB plate medium, each dilution was repeated twice, and cultured upside down in a 30°C incubator for 24 hours, a single colony was picked, and the plate was streaked and purified 3 times to obtain the cultured pure The strains are respectively inoculated on the PDA medium coated with American ginseng root rot fungus Fusarium solani, cultured at 28°C for 2-3 days, and the strains with obvious bacteriostatic zone are selected for purification and preservation.

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Embodiment 2

[0032] Embodiment 2, the antibacterial effect measurement of Paenibacillus polymyxa (Paenibacillus polymyxa) DN67

[0033] The present embodiment provides a kind of biocontrol bacterium agent, and described biocontrol bacterium agent is the fermented liquid of Paenibacillus polymyxa DN67, and its preparation method is as follows:

[0034] (1) inoculating the Paenibacillus polymyxa DN67 in a liquid medium and culturing it until the OD600 of the 20-fold dilution of the culture solution is 0.3 to 0.8 to obtain a seed solution;

[0035] Components and final concentration of the liquid medium: glucose 1.6%, sodium chloride 0.25%, magnesium sulfate 0.05%, yeast extract powder 1%, ammonium chloride 0.7%, dipotassium hydrogen phosphate 0.1%, pH7.

[0036] (2) The seed solution is 1-10% by volume. The amount of inoculation in the fermentation medium to expand the culture, the temperature of the expanded culture is 30 ° C, until the concentration of bacteria in the fermentation broth r...

Embodiment 3

[0039] Embodiment 3, Paenibacillus polymyxa (Paenibacillus polymyxa) DN67 is to the inhibitory effect of Fusarium solani spore germination

[0040] In 2% sucrose solution and Paenibacillus polymyxa DN67 fermentation supernatant, add the final concentration of 10 4 1 / mL of the spore suspension of Fusarium solani, each treatment group took 10 μL of the spore suspension and placed it on a glass slide, and placed it in a heat-preserving and moisturizing incubator for 10 hours at 28°C, and observed the spore germination of each treatment group , to calculate the germination rate. Microscopic observation found that the Fusarium solani spores in the fermentation supernatant of Paenibacillus polymyxa DN67 could not form germ tubes, and the spores appeared abnormal expansion and rupture, and the germination rate was 0, while the solani The germination rate of Fusarium spores was 98%.

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Abstract

The invention relates to a strain of paenibacillus polymyxa for resisting American ginseng root decay fusarium and application thereof, and belongs to the technical field of plant disease biological prevention and treatment, microbial resource development and utilization. The preservation number of the paenibacillus polymyxa is CGMCC No.15112. The application refers to the application of the paenibacillus polymyxa to resistance of fusarium and the prevention and treatment of American ginseng root decay diseases, watermelon fusarium wilt, wheat scab and solanaceae wilt. Compared with the existing farm insecticides and biological bacterium agents, the paenibacillus polymyxa DN67 has an efficient resistance effect on the fusarium.

Description

technical field [0001] The invention belongs to the technical field of biological control of plant diseases and the field of development and utilization of microbial resources, in particular to a Paenibacillus polymyxa and its application. Background technique [0002] American ginseng (scientific name: Panax quinquefolius) is a perennial plant of the genus Panax of the Araliaceae family. It is also known as American ginseng, American ginseng, American ginseng, and American ginseng. It is also planted in Changbai Mountain and other places. American ginseng has the dual effects of nourishing yin and nourishing qi, calming the mind and improving intelligence, clearing away heat and promoting body fluid, reducing fire and relieving summer heat. It is widely used and has a great market demand. However, the obstacles to continuous cropping of American ginseng are extremely serious. When American ginseng is continuously planted on the same land, it is often severely damaged by di...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 陈国忠冯志彬张兴晓李田田刘钺潘佳明
Owner LUDONG UNIVERSITY
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