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Glioma prognostic marker circ 7:73686636|73687095 and application

A prognostic marker, glioma technology, applied in biochemical equipment and methods, microbiological determination/testing, DNA/RNA fragments, etc.

Active Publication Date: 2018-04-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, even so, the recurrence rate of patients is still high, the 2-year survival rate is only 5%, and the average survival period of malignant glioblastoma is only about 14 months

Method used

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  • Glioma prognostic marker circ 7:73686636|73687095 and application
  • Glioma prognostic marker circ 7:73686636|73687095 and application
  • Glioma prognostic marker circ 7:73686636|73687095 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Preparation of reagents for detecting the expression of circRNA circ 7:73686636|73687095 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0020] 1. RNA stabilization solution 50ml

[0021] 2. Isopropanol 100ml

[0022] 3. Chloroform 100ml

[0023] 4. Trizol (from Molecular Research Center company) 50ml

[0024] 5. Enzyme-free water 10ml

[0025] 6.1 μM random reverse transcription primer (Thermo Company) 50 μl

[0026] 7.5 × reverse transcription buffer (Thermo company) 200ml

[0027] 8. 10mM base triphosphate deoxynucleotide (Thermo company) 100μl

[0028] 9.40U / μl RNase inhibitor (Thermo company) 500μl

[0029] 10.200U / μl MMLV reverse transcriptase (Thermo Company) 50μl

[0030] 11.Premix Ex Taq (Thermo Company) 50μl

[0031] 12.10μM circRNA circ 7:73686636|73687095 real-time fluorescence quantitative PCR specific primer 30μl

[0032] circRNA circ 7:73686636|73687095 Forward primer:

[0033] 5'-TTACCCTGATGGGTGTGGA...

Embodiment 2c

[0039] Example 2 The relationship between the expression level of circRNA circ 7:73686636|73687095 in glioma tissue and prognosis

[0040] 1. Preservation of glioma tissue: collect the glioma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0041] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After liquid, move to tube, add 200μl of chloroform to the tube, shake by hand for 15-30s, place on ice for 15min, centrifuge at 12000rpm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add pre-cooled isopropanol Mix with 0.5ml, put it on ice for 20min, centrifuge at 12000rpm at 4°C for 10min; discard the supernatant, add 1-2ml of ethanol diluted with 75% DEPC water, mi...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker circ 7:73686636|73687095 and application. A reagent for detecting the expression quantity of circRNA circ 7:73686636|73687095 of a glioma source is used for preparing a prognostic preparation of a glioma patient. Research proves that the glioma patient with relatively low expression of circRNA circ 7:73686636|73687095 has relatively high postoperative survival rate. A prognosis of the glioma patient is made by detecting the expression level of circRNA circ 7:73686636|73687095 in glioma tissue of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker for glioma prognosis, the application of a reagent for detecting the marker to prepare a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most common intracranial tumor with high incidence and high malignancy. The current treatment of glioma is mainly surgical resection, supplemented by radiotherapy and chemotherapy. However, even so, the recurrence rate of patients is still high, the 2-year survival rate is only 5%, and the average survival period of malignant glioblastoma is only about 14 months. One of the main factors that make glioma difficult to cure is the aggressive growth of glioma itself, and the boundary between tumor cells and normal brain tissue is very blurred and it is difficult to completely remove it by surgery; in addition, due to the rapid proliferation of glioma cells Tumor cells that survive in the hypoxic micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 付海娟武明花刘洋李沛瑶赵春花陈琼余志斌
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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