Primers for identification of a porcine epidemic diarrhea virus vaccine virus cv777 and wild strain

Abstract

The invention discloses porcine epidemic diarrhea virus vaccine strain and wild strain identification primers and a preparation method thereof, and belongs to the technical field of detection of porcine epidemic diarrhea virus; specifically, a PEDV vaccine strain CV777 and a wild strain are designed, and a differential gene sequence of the PEDV vaccine strain CV777 and the wild strain is GTCTTCCTGTGGCACCTGAAACAA. Correspondingly, the primers include Ps1, Pa1 and Pa2, wherein the sequence of the Ps1 is shown as ACACTATACTATCCCACCG, the sequence of the Pa1 is shown as TTTCAGGAGCTACATTARGA, and the sequence of the Pa2 is shown as CACCCAAAGATCCCAAGA; the length of an amplified fragment of a Ps1 / Pa1 primer pair is 505bp; and the length of the amplified fragment of a Ps1 / Pa2 primer pair is 793bp; and the preparation method provided by the invention comprises steps of RNA extraction, identification of the PEDV vaccine strain and the wild strain RT-PCR, specific test, sensitivity test and obtaining detection results.

Description

technical field [0001] The invention belongs to the technical field of detection of porcine epidemic diarrhea virus, in particular to a primer for identifying porcine epidemic diarrhea virus vaccine virus CV777 and wild virus strains. Background technique [0002] Porcine epidemic diarrhea disease is an acute intestinal infectious disease of pigs caused by Porcine Epidemic Diarrhea Virus and is characterized by watery diarrhea, vomiting and dehydration. The causative agent belongs to the genus Coronaviridae. Porcine epidemic diarrhea virus can persist in pig herds, and pigs of all ages are susceptible, especially suckling piglets, which are highly susceptible to infection, and the incidence rate can reach 100%. The mortality rate of suckling pigs within 7 days of age is as high as 100%, which has caused great economic losses to the pig industry. At present, PEDV infection is common and the epidemic range is wide, and the attenuated vaccine to prevent the disease is also wi...

AI Technical Summary

Problems solved by technology

The mortality rate of suckling pigs under the age of 7 days is as high as 100%, which has caused great economic losses to the pig industry

At present, PEDV infection is common and the epidemic range is wide, and attenuated vaccines to prevent the disease are also widely used. Antibody detection alone cannot truly reflect the immune status of pigs. Therefore, the differential diagnosis of PEDV vaccine strains and wild strains is very important for this disease Immunization and prevention of

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