Surface-enhanced Raman gold-labeled test paper strip for quickly detecting trace fipronil and preparation method thereof

A surface-enhanced Raman and micro-fipronil technology, which is applied in the direction of measuring devices, Raman scattering, instruments, etc., to achieve high sensitivity, consumer health protection, and high labeling rate
CN107941785AInactive Publication Date: 2018-04-20HENAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HENAN ACAD OF AGRI SCI
Publication Date
2018-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a surface-enhanced Raman gold-labeled test paper strip for quickly detecting trace fipronil and a preparation method thereof. A bottom layer of the test paper strip is a support layer, a middle layer of the test paper strip is an adsorption layer, a protection layer is fixed on the adsorption layer, and the adsorption layer sequentially comprises an absorption fibrous layer, a surface-enhanced Raman colloidal gold-labeled antibody fibrous layer, a cellulose membrane layer and a water absorption material layer of a handle end from a test end, wherein the cellulose membrane is coated with fipronil-coupled carrier protein to serve as a detection line and is also provided with a quality control line printed by goat or rabbit anti-mouse IgG (Immunoglobulin G), goat anti-rabbit IgG antibody or SPA (Staphylococcal Protein A). The test paper strip disclosed by the invention is prepared on the basis of a competitive mode, and target objects on a sample and the detectionline are competitively combined with the surface-enhanced Raman colloidal gold-labeled antibody. Tests show that the test paper has stronger specificity and higher sensitivity, the visual detection limit is an ng level, the quantitative detection limit of a Raman instrument is a pg level, and the test paper has no cross reaction with other pesticides or veterinary drugs.
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Description

technical field

[0001] The invention relates to a device for rapidly detecting trace amounts of pesticides, in particular to a surface-enhanced Raman gold standard test strip for rapidly detecting trace amounts of fipronil and a preparation method thereof. Background technique

[0002] Fipronil, trade name Fipronil, English common name Fipronil (fipronil), chemical name (RS)-5-amino-1-(2,6-dichloro-a,a,a-trifluoro-p -Tolyl)-4-trifluoromethylsulfinylpyrazole-3-carbonitrile, the molecular formula is C 8 N 2 Cl 4 , molecular formula C12H4Cl2F6N4OS, molecular weight 437.2, is a phenylpyrazole insecticide developed in 1981 by the French company Rhône-Planck. It has been used since the mid-1990s. It is a broad-spectrum insecticide and veterinary drug widely used in the world. It is mainly used for crop pest control, family pet parasite treatment and control, public health and entertainment venue pest control, etc. Widely used in all aspects. It binds to the γ-aminobutyric aci...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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