A method for producing Cordyceps militaris enzyme based on enzyme-fermentation coupling technology

Abstract

The invention provides a method for the production of Cordyceps militaris enzyme by enzyme-fermentation coupling. The production technology is based on the metabolic basis of two main combined enzyme preparations coupled with microbial fermentation to produce Cordyceps militaris enzyme, and adopts optimized conditions and reasonable techniques to make Cordyceps militaris The cell wall dissociates and transforms, and the active ingredients are fully released, coupled with microbial co-fermentation, to produce highly active enzymes and specific physiologically active substances, especially to improve the taste and digestibility of Cordyceps militaris, forming high-quality Cordyceps militaris enzymes. The technical invention is to biodegrade the cell wall of Cordyceps militaris through food-grade compound enzymes, coupled with the second-stage fermentation and separation of food-grade Lactobacillus plantarum to obtain liquid Cordyceps militaris enzymes, which can be used in food, beverages, seasonings and Addition of cosmetics and tobacco and wine materials.

Description

technical field

[0001] The invention relates to a method for producing cordyceps militaris enzyme based on an enzyme-enzyme coupling technology, and belongs to the technical field of enzyme preparation. Background technique

[0002] Cordyceps militaris is the type species of Ascomycota, Hypocystis, Ergotaceae and Cordyceps genus. scientific name Cordyceps militaris Cordyceps militaris, also known as Cordyceps militaris; as a new resource food, Cordyceps militaris is rich in cordycepin, cordyceps polysaccharides, cordycepic acid and a large number of nucleosides, amino acids, etc. It has good biological activity; Cordyceps militaris is a fungus (fungus; eumycetes), is Heterotrophic organisms with a nucleus and cell wall. The most characteristic cell wall of Cordyceps militaris is rich in chitin and cellulose. These cell wall structures cannot be degraded by the human body, making it difficult for the intracellular components of Cordyceps militaris to be released and absor...

Examples

Embodiment Construction

[0015] Include the following steps:

[0016] 1, Cordyceps militaris raw material processing: Cordyceps militaris raw material refers to Cordyceps militaris liquid fermentation mycelium and fungus powder, Cordyceps militaris fresh product and dry product, raw material processing comprises the following methods, fresh Cordyceps militaris by weight 1:5 ratio (100 kilograms of fresh Cordyceps militaris plus 500 kilograms of water) add water into the colloid mill for grinding and then transfer to the fermenter, sterilize at 121°C for 0.5 hours, and then cool down to 48±2°C for later use; dry Cordyceps militaris is in a ratio of 1:10 by weight (100 kg of dry pupa) Cordyceps plus 1000 kg of water) add water into the colloid mill for grinding and then transfer to the fermentation tank, pass through 121 ° C, sterilize for 0.5 hours, then cool down to 48 ± 2 ° C for standby; After sterilization, cool down to 48±2°C for standby use; add 800kg of water to 50kg of Cordyceps militaris ferme...

technical field

[0001] The invention relates to a method for producing cordyceps militaris enzyme based on an enzyme-enzyme coupling technology, and belongs to the technical field of enzyme preparation. Background technique

[0002] Cordyceps militaris is the type species of Ascomycota, Hypocystis, Ergotaceae and Cordyceps genus. scientific name Cordyceps militaris Cordyceps militaris, also known as Cordyceps militaris; as a new resource food, Cordyceps militaris is rich in cordycepin, cordyceps polysaccharides, cordycepic acid and a large number of nucleosides, amino acids, etc. It has good biological activity; Cordyceps militaris is a fungus (fungus; eumycetes), is Heterotrophic organisms with a nucleus and cell wall. The most characteristic cell wall of Cordyceps militaris is rich in chitin and cellulose. These cell wall structures cannot be degraded by the human body, making it difficult for the intracellular components of Cordyceps militaris to be released and absor...

Examples

Embodiment Construction

[0015] Include the following steps:

[0016] 1, Cordyceps militaris raw material processing: Cordyceps militaris raw material refers to Cordyceps militaris liquid fermentation mycelium and fungus powder, Cordyceps militaris fresh product and dry product, raw material processing comprises the following methods, fresh Cordyceps militaris by weight 1:5 ratio (100 kilograms of fresh Cordyceps militaris plus 500 kilograms of water) add water into the colloid mill for grinding and then transfer to the fermenter, sterilize at 121°C for 0.5 hours, and then cool down to 48±2°C for later use; dry Cordyceps militaris is in a ratio of 1:10 by weight (100 kg of dry pupa) Cordyceps plus 1000 kg of water) add water into the colloid mill for grinding and then transfer to the fermentation tank, pass through 121 ° C, sterilize for 0.5 hours, then cool down to 48 ± 2 ° C for standby; After sterilization, cool down to 48±2°C for standby use; add 800kg of water to 50kg of Cordyceps militaris ferme...