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Rapid induction method for calathea zebrine rhizome adventitious buds

A technology of velvet and induction method, which is applied in botany equipment and methods, soilless cultivation, cultivation, etc. It can solve problems such as difficulty in forming new plants, small number of adventitious buds, and long time for adventitious buds to improve reproduction efficiency And the effect of commodity flower quality

Active Publication Date: 2018-05-01
CHANGCHUN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the deficiencies and defects of the prior art, the present invention provides a rapid induction method for adventitious buds of velvet arrowroot rhizomes to solve the problem of long time for adventitious buds to occur after rooting and rooting of velvet arrowroot rhizomes, difficulty in forming new plants, and the number of adventitious buds. less problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Cut the rhizome of velvet arrowroot from the mother plant, cut it into a length of 3 cm to 4 cm, and use a knife to make a 0.3 cm deep incision every 1 cm on the epidermis; use cells to break the velvet arrowroot Centrifuge it in a centrifuge for 10 minutes, and take the supernatant of the narrow-leaf bottle of Xiaocao for later use;

[0026] Put the rhizome of velvet arrowroot into the intermittent immersion plant culture device; dilute the supernatant of Arrowroot Narrowia with purified water to obtain the liquid of Arrowroot Narrowia, the weight of the supernatant of Arrowroot and purified water Ratio is 1:5, injects the small-leaved bottle grass liquid into the intermittent immersion type plant culture device, and the immersion frequency of the intermittent immersion type plant culture device is set to be submerged for 10 minutes every 1 hour, that is, every 1 hour the velvet arrowroot rhizome will Submerge them in the liquid of Narrow-leaved Pinger Xiaocao for 10 m...

Embodiment 2

[0029] Cut the rhizome of velvet arrowroot from the mother plant, cut it into a length of 5 cm to 6 cm, and use a knife to make a 0.5 cm deep incision every 1 cm on the epidermis; use cells to break the velvet arrowroot Centrifuge it in a centrifuge for 20 minutes after making it into juice, and take the supernatant of Xiaocao in the narrow-leaf bottle for later use;

[0030] Put the rhizome of velvet arrowroot into the intermittent immersion plant culture device; dilute the supernatant of Arrowroot Narrowia with purified water to obtain the liquid of Arrowroot Narrowia, the weight of the supernatant of Arrowroot and purified water Ratio is 1:4, injects the small-leaf bottle grass liquid into the intermittent immersion type plant culture device, and the immersion frequency of the intermittent immersion type plant culture device is set to be submerged for 15 minutes every 1 hour, that is, every 1 hour the velvet arrowroot rhizome will Submerged in the liquid of Azalea chinensis...

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Abstract

The invention discloses a rapid induction method for calathea zebrine rhizome adventitious buds, and belongs to the technical field of flower and plant propagation. Calathea zebrine rhizomes are sheared off from a stock plant and cut into pieces of the length being 3-6 cm, and 0.2-0.5 cm deep cuts are formed in the epidermis every 1 cm through a cutter; ophioglossum thermale is juiced through a cell crusher and then centrifuged on a centrifugal machine for 10-20 min, a supernate of the ophioglossum thermale is taken for use; the obtained calathea zebrine rhizomes are placed into a temporary immersion type plant cultivator; the supernate of the ophioglossum thermale is attenuated through pure water and then injected into the temporary immersion type plant cultivator, the immersion frequencyof the temporary immersion type plant cultivator is immersed for 10-15 min per hour, and the cultivation temperature is set between 20 DEG C and 30 DEG C. The problems that in the cutting propagationprocess of the calathea zebrine rhizomes, the adventitious bud occurrence time is long and the number is small are solved.

Description

technical field [0001] The invention belongs to the technical field of flower propagation, and in particular relates to a method for rapidly inducing adventitious buds when promoting rhizome propagation of velvet arrowroot. Background technique [0002] Velvet arrowroot (Calathea leopardina) is a perennial evergreen herbaceous foliage plant belonging to the arrowroot family Arathea genus. It is difficult to obtain seeds of velvet arrowroot, and the propagation methods that have been studied and applied are mainly tissue culture and rhizome cutting propagation. [0003] Tissue culture is mainly used for laboratory research because of its high production cost and complicated operation, but is rarely used by flower growers in production. At present, the most widely used in production is still to use its rhizomes for cutting propagation. [0004] The disadvantages of the existing rhizome cutting propagation method: the cultivation period is long and the reproduction coefficien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G31/00
Inventor 马艳丽丁云峰汤松梅鲍勃聿张朕杨蕾
Owner CHANGCHUN UNIV
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