Application of arsenic oxidizing bacterium in repairing pollution of trivalent arsenic in paddy field
A technology of arsenic oxidizing bacteria and trivalent arsenic, applied in the field of environmental chemistry and environmental microorganisms, can solve the problems of secondary pollution, low efficiency, time-consuming, etc., achieve strong mobility, strong resistance and adaptability, and reduce harm Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] Example 1: Isolation of Achromobacter xylosoxidans YYS001 from arsenic-contaminated soil
[0027] The experimental soil was taken from arsenic-contaminated soil at the foot of Zijin Mountain in Shanghang County, Fujian Province. The enrichment, isolation, purification and genus identification of arsenic-oxidizing bacteria YYS001 were carried out according to the following steps.
[0028] (1) Enrichment of arsenic-resistant bacteria: Accurately weigh 1 g of arsenic-contaminated soil into a Erlenmeyer flask filled with 150 ml of sterilized enrichment medium containing 25 mg As(III) / L, culture on a shaker at 30°C (128 rpm) for one week to observe the strain growth. When the culture medium appeared turbid, inoculate it in the enrichment medium with the next higher concentration of As(III) according to the ratio of 10% (volume ratio), and the concentrations of As(III) were 50 mg / L and 100 mg / L respectively. L, 150 mg / L, 200 mg / L, 400 mg / L, in order to achieve the purpose of...
Embodiment 2
[0034] Example 2: Arsenic oxidation curve and growth curve of Achromobacter xylosoxidans YYS001
[0035] Pick the monoclonal strain of Achromobacter xylosoxidans YYS001, inoculate it into 100mL of the most suitable liquid medium E containing 100mgAs(III) / L, and place it in a shaker at 30°C for 48 hours. Draw 5mL to 100mL of fresh optimum liquid medium E containing 150mgAs(III) / L, place it in a shaker at 30°C for 24 hours, and then draw 5mL to 100mL of fresh optimum liquid containing 200mgAs(III) / L In culture medium E, shake culture in a shaker at 30°C for 24 hours, at this time the OD of YYS001 600 It is about 0.8, stored in a refrigerator at 4°C, and used as a seed solution for inoculation. Draw 5mL from the seed bacterial solution with 5% inoculation amount into 100mL fresh optimum liquid medium E containing 1000mgAs(III) / L, put it in a shaker at 30°C for 9 hours and start sampling after 9 hours, every 1 hour Take a sample until As(III) is completely oxidized, take 1.2mL a...
Embodiment 3
[0037] Example 3: Tolerance of Achromobacter xylosoxidans YYS001 to environmental pH and temperature
[0038] Draw 5mL from the above-mentioned seed bacterial solution with an inoculum amount of 5% to 100mL fresh optimal liquid medium E containing 1000mg As(III) / L, and place it in a shaking table with different pH values and different temperatures for 22 hours. After that, take a sample and measure the OD of YYS001 by spectrophotometry 600 , Take it now and do it now.
[0039] The specific method is as follows:
[0040] 1. The temperature of the fixed medium E is 35°C, placed in the medium E with a pH of 3.0~12.0, and after shaking for 22 hours, use the most suitable liquid medium E without YYS001 as the reference solution, and take each time 1.2mL, directly measure its absorbance at 600nm. The tolerance of YYS001 to the environmental pH value is shown in Table 1.
[0041] 2. The pH value of the fixed medium E is 8.5. Place it in the medium E at 25~60°C. After 22 hours o...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap