Method for identifying germplasm resource of radix codonopsis by use of ISSR (inter-simple sequence repeat) fingerprint

A technology of fingerprints and Codonopsis pilosula, which is applied to biochemical equipment and methods, and the determination/inspection of microorganisms, etc., can solve the problems of complex operation steps, high cost of identification methods, and long time consumption, and achieve the optimization of amplification procedures, simple and easy Effect of implementing and reducing workload
CN107974491AInactive Publication Date: 2018-05-01湖北省农业科学院中药材研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
湖北省农业科学院中药材研究所
Publication Date
2018-05-01
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for identifying the germplasm resource of radix codonopsis by use of ISSR (inter-simple sequence repeat) fingerprint. The method comprises the following steps: (1) collecting and numbering radix codonopsis from different production places; extracting the DNA of the radix codonopsis from different production places and performing PCR amplification; detecting amplification products by agarose gel electrophoresis; according to the result of agarose gel electrophoresis and by adopting the number of radix codonopsis as a horizontal coordinate and the length of DNA fragment as a longitudinal coordinate, recording the detection results of the DNA amplification stripes of the radix codonopsis from different production places at the intersections between the horizontal coordinate and the longitudinal coordinate respectively; marking the detection result with DNA amplification stripe, and not marking the detection result without DNA amplification stripe, so as toobtain the ISSR fingerprints of the radix codonopsis from different production places; (2) comparing the ISSR fingerprint of the to-be-detected radix codonopsis with the ISSR fingerprints of the radix codonopsis from different production places, and judging the production place of the to-be-detected radix codonopsis according to the comparison result. The method disclosed by the invention can beused for conveniently and quickly identifying the radix codonopsis medicinal materials with little difference in appearance and easily confused, and quick and accurate identification can be performedin the seedling stage of the radix codonopsis; therefore, the method is of great significance on guaranteeing the accuracy and reliability of the base source of a medicinal material.
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Description

technical field

[0001] The invention belongs to the technical field of quality detection of traditional Chinese medicinal materials, and relates to a method for identifying origins of Codonopsis pilosula, in particular to a method for identifying germplasm resources of Codonopsis pilosula using ISSR fingerprints. Background technique

[0002] Codonopsis pilosula is the dry root of Codonopsis pilosula (Franch.) Nannf), C.pilosula Nannf.var.modesta (Nannf.) L.T.Shen or C.tangshenOliv. The commonly used traditional Chinese medicine for medicine and food in my country has the effects of invigorating the spleen and lungs, nourishing blood and promoting body fluid. Driven by economic interests and limited by the level of species identification, Codonopsis pilosula is currently used in many places, and the value of different varieties is different. The aboveground parts of Codonopsis pilosula belong to closely related plants in appearance, and the types of active ingredients contai...

Claims

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