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Fermenting culture medium of bacillus and fermenting method

A fermentation medium and fermentation culture technology, applied in the field of fermentation engineering, can solve the problems of increased equipment investment, loss of fermentation metabolites, loss of fermentation broth, etc.

Inactive Publication Date: 2018-05-04
SICHUAN LOMON BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in addition to increasing the investment in equipment, centrifugal treatment also loses part of the fermentation broth due to the increase of fermentation solid content, resulting in the loss of fermentation metabolites

Method used

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  • Fermenting culture medium of bacillus and fermenting method
  • Fermenting culture medium of bacillus and fermenting method
  • Fermenting culture medium of bacillus and fermenting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0060] 1.1 Medium

[0061] 1.1.1 Seed activation medium

[0062] The seed activation medium is PDA medium, as shown in Table 1 below.

[0063] Table 1 Seed activation medium

[0064] components

Raw material specification

Dosage(g / L)

Potato

*

200

glucose

food grade

20

agar powder

Biochemical reagents

20

[0065] The pH is natural, and the pH before sterilization is 5.8-6.2, and this range is used as a reference for process parameters.

[0066] 1.1.2 Shake flask seed (first-class seed) culture medium

[0067] Seed shake flask culture medium is shown in Table 2 below.

[0068] Table 2 Shake Flask Medium

[0069]

[0070] 1.1.3 Fermentation medium

[0071] The fermentation medium is specifically shown in Table 3 below.

[0072] Table 3 Fermentation medium

[0073]

[0074]

[0075] 1.2 Instruments and equipment

[0076] Table 4 Instruments and equipment

[0077] Instruments and equipment

...

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Abstract

The invention provides a fermenting culture medium of bacillus. The fermenting culture medium comprises 30 to 80g / L of nitrogen source, 80 to 120g / L of carbon source, 1 to 4g / L of inorganic phosphate,and 1.5 to 5g / L of inorganic sulfate, wherein the carbon source comprises 60 to 100g / L of feeding carbon source. Compared with the prior art, the fermenting culture medium has the advantage that by adjusting the carbon and nitrogen ratio in the fermenting culture medium, the concentration of the bacillus is increased, the total amount of product is increased, and the content of solid in the fermenting process is increased.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a fermentation medium and a fermentation method of bacillus. Background technique [0002] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) is a Gram-positive bacterium that grows well on NA medium. The colonies are off-white, with irregular edges, rough surface, and rod-shaped microscopic examination with spores. [0003] Bacillus amyloliquefaciens has a wide range of applications in agriculture. It has obvious advantages in degrading chemical pesticide residues, preventing crop diseases, inhibiting bacteria and eliminating diseases, and improving crop quality and yield. The development of Bacillus amyloliquefaciens fermentation technology is in line with the country's agricultural needs requirements for sustainable development. In addition, in addition to the wide application in the agricultural field, the use in the aquaculture industry can...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/07
CPCC12N1/20
Inventor 刘健熊仁科左建英张俊景飞江黎欢
Owner SICHUAN LOMON BIO TECH CO LTD
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