Molecular marker relevant with soybean seed methionine contents, zone, primer and application

A seed methionine and molecular marker technology, applied in the field of soybean molecular biology, can solve the problems of undiscovered and genetically improved molecular marker-assisted selection breeding, etc., and achieve the effects of improving efficiency, reducing selection cost and improving selection efficiency.

Inactive Publication Date: 2018-05-04
JILIN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, no SNP (Single nucleotide polymorphism, single nucleotide polymorphism) molecular markers related to the methionine content traits of soybean seeds have been found, which brings difficulties to the genetic improvement of soybean seed methionine content traits and molecular marker-assisted selection breeding

Method used

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  • Molecular marker relevant with soybean seed methionine contents, zone, primer and application
  • Molecular marker relevant with soybean seed methionine contents, zone, primer and application
  • Molecular marker relevant with soybean seed methionine contents, zone, primer and application

Examples

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Embodiment Construction

[0023] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments, but should not be construed as a limitation of the present invention. Unless otherwise specified, the technical means used in the following examples are conventional means well known to those skilled in the art, and the materials, reagents, etc. used in the following examples, unless otherwise specified, can be obtained from commercial sources.

[0024] In the following examples, 3000 copies of soybean germplasm resources were widely collected in the north of 40°N in my country, and the content of methionine in soybean seeds was determined by high performance liquid chromatography. According to the distribution of methionine in the resources, each distribution group was sampled, and then Compare the genetic diversity of the sampling population and the total population, and determine the number of sampling samples when they are consistent. A total...

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Abstract

The invention belongs to the technical field of soybean molecular biology, and particularly relates to a molecular marker relevant with soybean seed methionine contents, a zone, a primer and application. The molecular marker is positioned at the 38617472th site of the No.2 chromosome of a soybean, and a nucleotide of the molecular marker is T or G; a nucleotide sequence containing the marker sequence is shown in SEQ ID NO.1; a marker zone is ranged from the 38517472th site to the 38717472th site of the No.2 chromosome of the soybean. The molecular marker belongs to a codominant marker, is reliable and is convenient to use, so that a great convenience is provided for performing selective breeding on varieties of high methionine contents of the soybeans, and the molecular marker can be applied to auxiliary selection of the molecular marker in improvement of soybean seed methionine content as well as fine location and map-based cloning of relevant genes of seed methionine content, so thatan improved progress of the soybean seed methionine content characters is accelerated.

Description

technical field [0001] The invention belongs to the technical field of soybean molecular biology, and in particular relates to a molecular marker, interval, primer and application related to the methionine content of soybean seeds. Background technique [0002] Soybean protein is one of the important sources of nutrition for humans and poultry. Its amino acid composition is similar to that of milk protein. It is a relatively complete protein, except for the lack of methionine necessary for human body. The quality of soybean protein affects the market competitiveness of soybean products, and the content of methionine is the primary limiting factor. The methionine content in soybeans is generally only 12mg / g protein, which is far from the 26mg / g recommended by the World Health Organization and the Chinese Nutrition Society, which meets the nutritional and health needs of the human body. In order to meet people's increasing nutritional needs, increasing the methionine content ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 邱红梅王跃强王洋侯云龙马晓萍高淑芹陈健胡金海王丽
Owner JILIN ACAD OF AGRI SCI
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