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AGTR1 gene detection kit and AGTR1 gene detection method

A gene detection and kit technology, applied in the fields of genetic engineering and molecular biology, can solve the problems of low sensitivity, high false positive rate and long period of AGTR1 gene detection, improve specificity and sensitivity, reduce false positive rate, The effect of short detection period

Inactive Publication Date: 2018-05-11
GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to provide an AGTR1 gene detection kit and detection method, aiming to solve the technical problems of low detection sensitivity, high false positive rate, long cycle and low throughput of AGTR1 gene detection in the prior art

Method used

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  • AGTR1 gene detection kit and AGTR1 gene detection method

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no. 1 example

[0025] The present invention proposes a first embodiment, an AGTR1 gene detection kit, comprising a primer set for specific amplification of nucleic acid fragments containing the rs5186 site, said primer set including upstream primers SEQ ID NO: 1, SEQ ID NO: 1, SEQ ID NO: ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and downstream primers SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8.

[0026] The AGTR1 gene detection kit provided by this program includes a specific amplification primer set, which can specifically amplify nucleic acid fragments containing the rs5186 site, reducing the possibility of amplifying non-target fragments, thereby The detection sensitivity of the rs5186 site is high, and the false positive rate is low.

[0027] Preferably, the kit further includes a linker, the linker includes at least one of linker A, linker B, linker C, and linker D; the linker A consists of SEQ ID NO: 9 and SEQ ID NO: 10; The joint B consists of SEQ ID NO:11 and SEQ ID NO:12; the ...

no. 2 example

[0032] The present invention proposes a second embodiment, a gene detection method, comprising the following steps:

[0033] A. Utilize the amplification primer set to amplify the sample containing the rs5186 site to obtain an amplified product; it is characterized in that the primer set includes upstream primers SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO : 3 or SEQ ID NO: 4, and downstream primers SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7 or SEQ ID NO: 8;

[0034] B. Connecting adapters to the amplification products to obtain library molecules;

[0035] C. Sequencing the library molecules by using the second-generation high-throughput gene sequencing technology to determine the genotype of the rs5186 site.

[0036] The gene detection method provided by the invention uses a specific amplification primer set to amplify the nucleic acid fragment containing the rs5186 site, thereby improving the detection sensitivity of the rs5186 site and reducing the false positive rate of detection.

...

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Abstract

The invention relates to the fields of genetic engineering and molecular biology, and discloses an AGTR1 gene detection kit. The detection kit comprises a primer group for specifically amplifying a nucleic acid fragment containing an rs5186 site, the primer group comprises an upstream primer represented by SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 or SEQ ID NO:4, and a downstream primer represented bySEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7 or SEQ ID NO:8. The invention also provides a gene detection method. The kit uses the specific amplification primer group to amplify the nucleic acid fragment containing the rs5186 site, so the detection sensitivity of AGTR1 gene mutation is high, and the false positive rate is low; and the kit adopts a second generation high-throughput gene sequencing technology to detect, so short detection period and high throughput are achieved.

Description

technical field [0001] The invention relates to the fields of genetic engineering and molecular biology, and more specifically relates to an AGTR1 gene detection kit and detection method. Background technique [0002] Hypertension is a disease caused by the interaction of genetic factors and environmental factors. Studies have shown that the renin-angiotensin system (RAS) plays an important role in regulating blood pressure and maintaining water and electrolyte balance in the body, and is involved in the occurrence and development of essential hypertension. The main biologically active substance of this system, angiotensin Ⅱ (angiotensin Ⅱ, AT Ⅱ) plays an important role in the occurrence and development of cardiac hypertrophy, and it plays a role mainly through the mediation of its type I receptor (angiotensin Ⅱ type1 receptor, AGTR1). its role. [0003] The AGTR1 gene is located on human chromosome 3q21-25, and its encoded product mainly mediates the cardiovascular effect...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12Q1/6869
CPCC12Q1/6869C12Q1/6888C12Q2600/106C12Q2600/156C12Q2525/191C12Q2535/122C12Q2521/301
Inventor 盛司潼高虹
Owner GUANGZHOU KANGXINRUI GENE HEALTH TECH CO LTD
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