Method for separating sheep muscle stem cells

A separation method and stem cell technology, applied in cell dissociation methods, animal cells, vertebrate cells, etc., can solve the problems of muscle stem cell separation troubles, short cell survival time, affecting research and treatment, and achieve low infection rate, Easy to train, effects for speed and efficiency

Inactive Publication Date: 2018-05-18
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Muscle stem cells are an indispensable culture object for studying how to repair damaged muscle tissue and how to effectively treat urinary incontinence. However, the separation of muscle stem cells in the prior art is more troublesome, and the survival time of cells after separation is short, which affects research and treatment.

Method used

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Embodiment Construction

[0015] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0016] A method for isolating sheep muscle stem cells, comprising the steps of:

[0017] S1. Select sheep of 2-3 years old, clean the sheep and lead them to the sterile room, remove the hair on the thighs, wrap the rest with sterile gauze, wash the thighs 2-3 times with alcohol, and take out the thigh muscles 4- 8g, soaked in alcohol;

[0018] S2. Alcohol disinfection for 10-20 minutes, remove the connective tissue and blood cells on the thigh muscles, and wash with normal saline to ensure the integrity of the cells. Clean the sheep thigh muscles;

[0019] S3, chopping the washed sheep thigh muscle in S2 to 0.8-1.2mm...

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Abstract

The invention discloses a method for separating sheep muscle stem cells. According to the method, muscle cells of the legs of a sheep are cut and obtained, the muscle cells are disinfected, cleaned, chopped, digested, centrifugally separated and the like, the muscle stem cells are separated and placed in a petri dish for culture; compared with conventional methods for separating the muscle stem cells, the method has the advantages that the separation efficiency is high, the infection rate in the separation process is low, the separated stem cells are high in survival rate and easy to culture,the number of the stem cells is increased, the muscle repair speed in the process of using the stem cells for treatment is increased, and the muscle repair efficiency in the process of using the stemcells for treatment is improved.

Description

technical field [0001] The invention belongs to the field of cell separation, and more specifically relates to a method for separating sheep muscle stem cells. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are not fully differentiated and immature cells, which have the potential to regenerate various tissues and organs and the human body, and are called "universal cells" in the medical field. Muscle stem cells can develop and differentiate into myoblasts, which can fuse with each other to form multinucleated muscle fibers, forming the most basic stru...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0775
CPCC12N5/0668C12N2509/00
Inventor 赵俊星秦旭泽张婷张瑞鑫王建新
Owner SHANXI AGRI UNIV
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