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A kind of snp marker of rice lodging resistance gene and its application

A technology for lodging and rice resistance, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of difficult and accurate determination of phenotype values, few SNP markers, and few major genes, and saves money Breeding time, avoiding the use of toxic substances, reducing the effect of pollution

Active Publication Date: 2020-11-13
HUAZHI RICE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, since the trait of lodging resistance is controlled by quantitative traits, and the phenotypic value is not easy to be accurately determined, there are relatively few main effect genes cloned at present, and there are relatively few SNP markers that are actually designed for the main effect genes
SNP molecular markers for rice lodging resistance are basically designed based on QTL, so such SNP markers may be less valuable in actual breeding

Method used

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  • A kind of snp marker of rice lodging resistance gene and its application
  • A kind of snp marker of rice lodging resistance gene and its application
  • A kind of snp marker of rice lodging resistance gene and its application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] According to literature data, the rice SCM3 gene was located on the third chromosome with reference to the physical location of Nipponbare: 28428504-28430438 interval. The gene interval was expanded to both sides by 50kb, and SNP sites were extracted based on 3,000 rice resequencing data from the International Rice Institute, and the sequencing results of 8 materials with clear phenotypes were analyzed by referring to Huazhi Rice Biotechnology Co., Ltd. , the SNP information of these 9 materials can be summarized as follows, as shown in Table 2:

[0044] Table 2 SNP information of 9 rice samples in the experiment

[0045]

[0046]

[0047] Among them, SNP6 is the differential site reported in the article, and SNP1, SNP2, SNP4, and SNP5 are linkage variant sites, so the above materials can be classified into three haplotypes (Haplotype), among which R900 is related to the donor who cloned the gene Chugoku117 is the same haplotype. According to the above SNP diffe...

Embodiment 2

[0054] According to the article on rice SCM3 cloning and genotyping results, we selected two pairs of parental materials (R900, Tetep and R900, Huahui 705) with differences in these three SNPs for hybridization, and selected positive F1 single plants, About 500 seeds were harvested in each combination, and the phenotypes of F2 families were used to verify the candidate markers. At the same time, DNA was extracted from F2 leaves, genotyping was carried out, and the three SNPs initially determined above were verified again in combination with phenotype values. The results proved that the three markers K_030531, K_030532, and K_030533 were functional marker combinations.

[0055] The phenotypic verification results of the R900×Tetep F2 generation are shown in Table 4. When the detection results of the three SNP markers K_030531, K_030532, and K_030533 are C, T, and G, respectively, the individual plant is detected as lodging-resistant SCM3 genotype (R) ; When the three SNP marker...

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Abstract

The invention provides a primer group used for detecting a rice lodging-resistant gene SCM3. The gene SCM3 comprises three SNP molecular markers which are respectively K_030531, K_030532 and K_030533,wherein the polymorphic basic group of the K_030531 is C or T, the polymorphic basic group of the K_030532 is T or C, and the polymorphic basic group of the K_030533 is C or G. The molecular markerscan be used to rapidly accurately detect the gene SCM3, so that the transformation efficiency of the gene is greatly improved. The detection process does not need digestion, electrophoresis, sequencing and the like, the operation is convenient and is favorable for the high-throughput rapid detection, and the aerosol pollution of a PCR product, the environment pollution caused by EB, and the hazardof formaldehyde on the human body are completely eliminated.

Description

Technical field: [0001] The invention relates to the field of molecular markers, in particular to a SNP molecular marker of a rice lodging resistance gene. Background technique: [0002] With the breeding and popularization of high-yielding rice in recent years, rice lodging has become a common phenomenon. Rice lodging seriously affects the yield and variety of rice, and increases the difficulty and cost of harvesting. Rice lodging resistance traits are controlled by quantitative trait loci (QTL), and currently only a few cloned lodging resistance genes are actually used for genetic improvement and breeding of rice. [0003] Traditional pedigree breeding is the most common rice breeding method in recent years, and a large number of high-yield and high-quality rice varieties have been born as a result. However, the traditional breeding method of hybridization combined with phenotype often needs to increase the backcross population because of the inaccurate grasp of the phen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/6895C12Q1/6858
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 姚文元彭佩李文博吴云天郑秀婷
Owner HUAZHI RICE BIO TECH CO LTD