A large-scale extraction method of egg yolk antibody
A technology of egg yolk antibody and extraction method, applied in the field of large-scale egg yolk antibody extraction, can solve the problems of short product shelf life, low product purity, long production cycle, etc. Effect
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Embodiment 1
[0026] A large-scale method for extracting egg yolk antibodies, comprising the steps of:
[0027] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies against gosling plague in 0.1% bromogeramine disinfectant for 15 minutes, then fumigate them with formaldehyde for 30 minutes;
[0028] 2) Manually or mechanically beat the sterilized 20,000 high-free eggs to separate the yolk;
[0029] 3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:1.5; continue stirring for 25 minutes, and stir The speed is 450r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.0, then stop the dripping;
[0030] 4) Add an appropriate amount of octanoic acid solution with a concentration of 1.5% to the egg yolk ob...
Embodiment 2
[0035] A large-scale method for extracting egg yolk antibodies, comprising the steps of:
[0036] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies of gosling plague with 0.1% bromogeramine disinfectant for 15 minutes, and then fumigate them with formaldehyde for 30 minutes;
[0037] 2) Manually or mechanically beat 20,000 sterilized high-free eggs to separate the yolk;
[0038]3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:2; continue stirring for 27 minutes, and stir The speed is 500r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.1, and then stop the dripping;
[0039] 4) Add an appropriate amount of octanoic acid solution with a concentration of 2.25% to the egg yolk obt...
Embodiment 3
[0044] A large-scale method for extracting egg yolk antibodies, comprising the steps of:
[0045] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies against gosling plague in 0.1% bromogeramine disinfectant for 15 minutes, then fumigate them with formaldehyde for 30 minutes;
[0046] 2) 20,000 sterilized high-free eggs are manually or mechanically beaten to separate the yolk;
[0047] 3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:3; continue stirring for 35 minutes, and stir The speed is 550r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.2, stop the dripping;
[0048] 4) Add an appropriate amount of octanoic acid solution with a concentration of 3% to the yolk obtained in s...
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