A large-scale extraction method of egg yolk antibody

A technology of egg yolk antibody and extraction method, applied in the field of large-scale egg yolk antibody extraction, can solve the problems of short product shelf life, low product purity, long production cycle, etc. Effect

Active Publication Date: 2021-02-19
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned problems in the prior art, the present invention proposes a large-scale method for extracting egg yolk antibodies, which solves the problems of long production cycle, high production cost, and high content of yolk antibody products obtained by existing egg yolk extraction methods. Polymeric proteins lead to technical problems such as low purity of the product and short shelf life of the product

Method used

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  • A large-scale extraction method of egg yolk antibody
  • A large-scale extraction method of egg yolk antibody
  • A large-scale extraction method of egg yolk antibody

Examples

Experimental program
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Embodiment 1

[0026] A large-scale method for extracting egg yolk antibodies, comprising the steps of:

[0027] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies against gosling plague in 0.1% bromogeramine disinfectant for 15 minutes, then fumigate them with formaldehyde for 30 minutes;

[0028] 2) Manually or mechanically beat the sterilized 20,000 high-free eggs to separate the yolk;

[0029] 3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:1.5; continue stirring for 25 minutes, and stir The speed is 450r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.0, then stop the dripping;

[0030] 4) Add an appropriate amount of octanoic acid solution with a concentration of 1.5% to the egg yolk ob...

Embodiment 2

[0035] A large-scale method for extracting egg yolk antibodies, comprising the steps of:

[0036] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies of gosling plague with 0.1% bromogeramine disinfectant for 15 minutes, and then fumigate them with formaldehyde for 30 minutes;

[0037] 2) Manually or mechanically beat 20,000 sterilized high-free eggs to separate the yolk;

[0038]3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:2; continue stirring for 27 minutes, and stir The speed is 500r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.1, and then stop the dripping;

[0039] 4) Add an appropriate amount of octanoic acid solution with a concentration of 2.25% to the egg yolk obt...

Embodiment 3

[0044] A large-scale method for extracting egg yolk antibodies, comprising the steps of:

[0045] 1) Soak and disinfect 20,000 high-free eggs used to extract yolk antibodies against gosling plague in 0.1% bromogeramine disinfectant for 15 minutes, then fumigate them with formaldehyde for 30 minutes;

[0046] 2) 20,000 sterilized high-free eggs are manually or mechanically beaten to separate the yolk;

[0047] 3) Transfer the egg yolk separated in step 2) into a mixing tank, stir the egg yolk into a paste, then add water for injection into the mixing tank, the volume ratio of the egg yolk to water for injection is 1:3; continue stirring for 35 minutes, and stir The speed is 550r / min, after the stirring is completed; then gradually add an appropriate amount of acetic acid-sodium acetate buffer solution to the egg yolk until the pH value is 5.2, stop the dripping;

[0048] 4) Add an appropriate amount of octanoic acid solution with a concentration of 3% to the yolk obtained in s...

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Abstract

The invention discloses a large-scale method for extracting egg yolk antibodies, which comprises the following steps: separating egg yolks after sterilizing high-free eggs; stirring the separated egg yolks into a paste, adding water for injection, and the volume ratio of egg yolks to injection water is 1 : 1.5‑1: 3; then adjust the pH value of the egg yolk liquid to 5.0‑5.2; then add octanoic acid solution with a concentration of 1.5‑3%, stir evenly and then stand still for 2‑3 hours to filter the egg yolk liquid, when 80% of the lower layer After the clear liquid is filtered, stir the remaining liquid evenly and continue to filter to collect the filtrate; then add an appropriate amount of water for injection to dilute the filtrate to obtain a clear liquid, then adjust the pH value of the clear liquid to 6.0-7.0, and then add Formaldehyde solution with a final concentration of 0.05%, the clear liquid is filtered again, the filtrate is collected for sub-packaging and then inspected. After passing the inspection, it is labeled and transferred to the finished product warehouse. The invention significantly shortens the production cycle of the egg yolk antibody, reduces the production cost, has higher product purity and prolongs the shelf life of the product.

Description

technical field [0001] The invention relates to the field of egg yolk antibody extraction, in particular to a large-scale egg yolk antibody extraction method. Background technique [0002] Yolk antibody (yolk antibody, IgY) is formed when B cells of laying hens are stimulated by antigens to differentiate into plasma cells, secrete specific antibodies into the blood circulation, and accumulate in the yolk. The extracted yolk antibody can be used for the prevention and treatment of corresponding diseases . [0003] The extraction of egg yolk antibody has following several methods now: 1, chloroform extraction method, because the chloroform consumption of this method is very large, not only cause the cost height that egg yolk antibody extracts, and the purity of the egg yolk antibody product that obtains is low, and chloroform has relatively Strong toxicity; 2. Acidified water extraction method, the product of this method has many impurities, extremely unstable performance, an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/02
CPCC07K16/02
Inventor 赵光伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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