Method for researching methanogenesis metabolic pathway by utilizing selective depressants

Abstract

The invention discloses a method for researching a methanogenesis metabolic pathway by utilizing selective depressants. In an anaerobic digestion system for ammonia nitrogen-tolerant sludge, a methodfor researching the methanogenesis metabolic pathway of the anaerobic digestion system through different depressants is utilized; different anaerobic digestion methanogenesis pathways are depressed byadopting depressants BES (Dibromoethane Sulfonate), fluoroacetate and lumazine; a methanogenesis pathway of the anaerobic digestion system can be indirectly analyzed and judged according to the changes of a methane yield and VFAs (Volatile Fatty Acids), and meanwhile, the contribution rate of an AM (Aceticlastic Methanogenesis) pathway and an SAO-HM (Syntrophic Acetate Oxidation-HydrogenotrophicMethanogenesis) pathway to methanogenesis and the significance of an SAO process is clear and definite.

Description

technical field [0001] The invention relates to the fields of organic waste biological treatment technology and environmental protection purification treatment technology, in particular to a method for using selective inhibitors to study methane-producing metabolic pathways. Background technique [0002] Anaerobic fermentation technology is one of the more reasonable treatment technologies for organic solid waste or organic wastewater. However, under anaerobic conditions, the organic matter is gradually decomposed into small molecular organic matter and finally transformed into methane, which is a complex process completed by a variety of microorganisms in cooperation with each other. First, complex organic substances such as cellulose, protein, and lipids are first degraded into soluble small molecules under the action of strict anaerobic bacteria; acidogenic bacteria then convert small molecules into volatile fatty acids (VFAs), and VFAs are converted by hydrogen-producing...

AI Technical Summary

Problems solved by technology

However, the main problem faced by this technology in the application process is the relatively high cost

Selective inhibition of different metabolic pathways using inhibitors can also achieve quantitative research on metabolic mechanisms. At present, the application of selective inhibition methods in methanogenic metabolic pathways has been widely accepted. However, different anaerobic digestion processes, Due to the different metabolic pathways, there are large differences in the concentration of inhibitors

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