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A kind of oil palm somatic cell suspension culture obtains the method for regeneration plant

A technology for suspension culture and plant regeneration, applied in the biological field, can solve the problems such as no research report on oil palm somatic cell suspension culture, and achieve the effects of shortening the breeding cycle of varieties, short plant cycle and convenient operation.

Active Publication Date: 2019-12-03
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no research report on oil palm somatic cell suspension culture in China

Method used

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  • A kind of oil palm somatic cell suspension culture obtains the method for regeneration plant
  • A kind of oil palm somatic cell suspension culture obtains the method for regeneration plant
  • A kind of oil palm somatic cell suspension culture obtains the method for regeneration plant

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Fragile embryogenic callus induction

[0024] Get mature oil palm core leaf explants and cut them into small pieces of 1cm × 2cm, inoculate them in the embryogenic callus induction medium, cultivate them in the dark at 28°C, and obtain fragile embryogenic callus in 110 days (see figure 1 ).

[0025] Embryogenic callus induction medium: NH 4 Cl 1105mg / L+H 3 BO 3 6.2mg / L+KNO 32500mg / L+Ca(NO 3 ) 2 600mg / L+NaH 2 PO 4 150mg / L+MgSO 4 ·7H 2 O 370mg / L+CuSO 4 ·5H 2 O 0.025mg / L+Na 2 -EDTA37.3mg / L+FeSO 4 ·7H 2 O 27.8mg / L+MnSO 4 4H 2 O 22.3mg / L+ZnSO 4 ·5H 2 O 8.6mg / L+KI0.83mg / L+Na 2 MoO 4 2H 2 O 0.25mg / L+CoCl 2 ·6H 2 O 0.025mg / L+inositol 100mg / L+niacin 0.5mg / L+pyridoxine hydrochloride 5mg / L+thiamine hydrochloride 5mg / L+aminoacetic acid 2.0mg / L+dequamycin 5mg / L+30mg / L dicamba+ Activated carbon 1000mg / L+6000mg / L agar+sucrose 45000mg / L+calcium pantothenate 0.5mg / L+biotin 0.5mg / L+hydrolyzed casein 500mg / L, the pH of the medium was adjusted to 5.6 before st...

Embodiment 2

[0033] 1. Fragile embryogenic callus induction

[0034] The core leaf explants of mature oil palm were cut into small pieces of 1 cm×2 cm, inoculated into embryogenic callus induction medium, cultured in the dark at 30°C, and fragile embryogenic callus was obtained after 130 days.

[0035] Embryogenic callus induction medium: NH 4 Cl 1100mg / L+H 3 BO 3 6mg / L+KNO 3 2490mg / L+Ca(NO 3 ) 2 585mg / L+NaH 2 PO 4 160mg / L+MgSO 4 ·7H 2 O 350mg / L+CuSO 4 ·5H 2 O 0.027mg / L+Na 2 -EDTA 36.3mg / L+FeSO 4 ·7H 2 O 27.3mg / L+MnSO 4 4H 2 O 22.6mg / L+ZnSO 4 ·5H 2 O 8.6mg / L+KI8.7mg / L+Na 2 MoO 4 2H 2 O0.27mg / L+CoCl 2 ·6H 2 O 0.024mg / L+inositol 80mg / L+niacin 0.8mg / L+pyridoxine hydrochloride 10mg / L+thiamine hydrochloride 10mg / L+aminoacetic acid 2.0mg / L+dequamycin 7.5mg / L+20mg / L dicamba +Activated carbon 2000mg / L+6g / L agar+sucrose 45000mg / L+calcium pantothenate 0.5mg / L+biotin 0.5mg / L+hydrolyzed casein 750mg / L, the pH of the medium was adjusted to 5.6 before sterilization.

[0036] ...

Embodiment 3

[0043] 1. Fragile embryogenic callus induction

[0044]The core leaf explants of mature oil palm were cut into small pieces of 1 cm×2 cm, inoculated into embryogenic callus induction medium, cultured in the dark at 28°C, and fragile embryogenic callus was obtained after 120 days.

[0045] Embryogenic callus induction medium: NH 4 Cl 1200mg / L+H 3 BO 3 7mg / L+KNO 3 2510mg / L+Ca(NO 3 ) 2 615mg / L+NaH 2 PO 4 140mg / L+MgSO 4 ·7H 2 O 390mg / L+CuSO 4 ·5H 2 O 0.023mg / L+Na 2 -EDTA38.3mg / L+FeSO 4 ·7H 2 O 28.3mg / L+MnSO 4 4H 2 O 22.2mg / L+ZnSO 4 ·5H 2 O 8.0mg / L+KI0.79mg / L+Na 2 MoO 4 2H 2 O 0.23mg / L+CoCl 2 ·6H 2 O 0.026mg / L+inositol 120mg / L+niacin 0.2mg / L+pyridoxine hydrochloride 15mg / L+thiamine hydrochloride 15mg / L+aminoacetic acid 2.0mg / L+dequamycin 10mg / L+15mg / L dicamba+ Activated carbon 1000mg / L+6000mg / L agar+sucrose 45000mg / L+calcium pantothenate 5mg / L+biotin 0.5mg / L+hydrolyzed casein 1000mg / L, the pH of the medium was adjusted to 5.6 before sterilization.

[004...

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Abstract

The invention belongs to the technical field of biology and relates to a method of producing regenerated plants through suspension culture of body cells of oil palm. In the method, core leaves of theoil palm is employed as an explant material and is directly induced to produce fragile embryonic callus; then embryogenic cell is reproduced to a large quantity through the cellular suspension culture; finally the embryogenic cell is cultured into a complete plant. The method has simple process and convenient operation, is short in period of acquiring clone plants and is high in reproductive efficiency. In the method, the suspension cell line has consistent source and clone, so that growth conditions are easy to control and physiological status is basically equal. The method has higher speed and consistency in various reactions, and the plants have excellent cell totipotency; the method has practical significance of high-effective breeding of excellent planting material of oil palm and reducing variety breeding period when being applied to biological seed breeding.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for obtaining regenerated plants by suspension culture of plant somatic cells, in particular to a method for obtaining regenerated plants by suspension culture of oil palm somatic cells. Background technique [0002] Oil palm is native to tropical Africa, mainly distributed in Malaysia, Indonesia, west and central Africa in Asia, northern South America and Central America. my country has introduced oil palm for many years, mainly distributed in Hainan, Yunnan and Guangdong It is the world's largest oil-producing crop. Its oil can be used as edible oil, industrial raw material, bio-energy, etc. It has a wide range of uses and a large market demand. It is also one of the key woody oil crops developed in my country. The environment in my country's thermal regions is complex and diverse, and there are no suitable domestic cultivars at present. Direct introduction and tradi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 潘登浪李炜芳邹积鑫曾宪海林位夫李哲曾精
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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