41results about How to "Growth conditions are easy to control" patented technology

The invention provides application of photosynthetic bacteria fermentation broth in pesticide and metal combined contamination soil. In the application, the photosynthetic bacteria fermentation broth with the number effective viable bacteria to be between 108 and 1,010 per millimeter is diluted to 10 to 150 times with water; the fermentation broth is uniformly sprayed to degrading bacteria treated soil in dosage of between 10 and 50kg per mu; the earth surface is turned over for about 20 cm after the spraying is completed to ensure that the broth and the soil are sufficiently mixed and the photosynthetic bacteria can degrade residues of carbamic acid ester pesticide in the soil in high efficiency; and simultaneously, the bioavailability of the metal can be reduced to repair the carbamic acid ester pesticide and metal combined contamination soil. The photosynthetic bacteria has wide raw material sources, low price and easily controlled growing condition, can use low molecular organic substances as photosynthetic electron receptor to perform photo heterotrophic growth under anaerobic illuminating condition, can use organic substances as breathing substrate to perform aerobic or heterotrophic growth under dark and aerobic conditions, can carry out the photosynthesis without releasing oxygen, and is suitable for large-scale industrial implementation.

The invention discloses a solar blind ultraviolet photoelectric detector based on an amorphous gallium oxide film and a preparation method thereof, and belongs to the technical field of photoelectricdetectors. The method comprises the steps that the crystal face (0001) Al2O3 is adopted as a substrate, and the substrate is cleaned; then, the cleaned substrate is fed into a settling chamber, a radio frequency magnetic control sputtering technology is applied to the substrate to grow a gallium oxide film; finally, a hollow interdigital mask plate is used for shielding on the amorphous gallium oxide film, a direct current magnetic control sputtering method is adopted for sputtering an interdigital metal electrode on the interdigital mask plate to obtain the solar blind ultraviolet photoelectric detector, the structure is an MSM type sandwiched structure, and the Al2O3 substrate, the amorphous gallium oxide film material and the Ti/Au interdigital metal electrode are arranged from bottom to top. The manufacturing technology is simple, the repeatability is good, dark current is small, the stability is high, the response speed is high, the ultraviolet visible restrain ratio is high, andthe detector conforms to the energy-saving and emission-reducing theory, is suitable for large-scale production, and has the wide development prospect.

The invention relates to Bacillus amyloliquefaciens and a method for producing chymosin by fermenting using the same and belongs to the technical field of microbial fermentation. The invention discloses a bacterial strain for producing the chymosin. The bacterial strain is named as Bacillus amyloliquefaciens JUN002 and is collected in China Center for Type Culture Collection, wherein the collection number is CCTCC No:M2011045. The method for producing the chymosin by using the bacterial strain through liquid fermentation comprises the following steps of: performing slant culture; (2) performing seed culture; and (3) performing liquid fermentation culture, namely inoculating cultured seeds into a fermentation culture medium according to the inoculation quantity of 0.1 to 0.2 percent to culture the seeds at the temperature of between 32 and 36 DEG C for 60 to 72 hours. The Bacillus amyloliquefaciens and the method for producing the chymosin by fermenting using the same have the advantages that: the chymosin is produced by using the Bacillus amyloliquefaciens CCTCC No:M2011045 through liquid fermentation, so the enzyme production level is high, the fermenting time is short, growth conditions are easy to control and the like; and the method is completely suitable for application to industrialized production.

The invention provides a method and application of a photosynthetic bacteria culture solution in medicinal material planting. The method comprises the following steps: diluting the photosynthetic bacteria culture solution; and evenly irrigating soil, soaking seeds, irrigating roots and spraying. By utilizing the method, the medicinal material growth can be promoted; the occurrence of various disasters can be prevented and treated; the problem of manual culture quality reduction caused by utilizing chemical fertilizers and pesticides can be solved; the traditional Chinese medicine extract content can be increased; the content of the effective components in the traditional Chinese medicinal material is increased; the heavy metal content is reduced; the utilization of the Chinese medicinal material is improved; the sustainable utilization of the Chinese medicinal material is realized; the resources are protected; the implementation of GAP (good agriculture practice) in China can be improved; the problem that crop rotation can not be performed in Chinese medicinal material planting can be solved; and the fertilizer amount is saved by one third.

The invention relates to a strain of bacillus subtilis and a method for producing chymosin by using the same, belonging to the technical field of microbial fermentation. The invention discloses a bacterial strain producing chymosin, which is named as bacillus subtilis T and is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCCNO: M2010259. A method for applying the bacterial strain to produce the chymosin through liquid fermentation comprises the following steps of: (1) slant culture; (2) seed culture; and (3) liquid fermentation culture: inoculating cultured seeds into a fermentation culture medium according to the inoculation quantity of 0.1-0.2%, culturing for 96-136 h at 32-36 DEG C, and controlling the rotating speed of a shaker at 150 rpm. Bacillus subtilis is common nonpathogenic bacterium and is applicable to chymosin production; the method for producing the chymosin by using the bacillus subtilis CCTCCNO: M2010259 through liquid fermentation has the advantages of high chymosin production level, short production period, low production cost, easily controlled growth conditions, convenient chymosin extraction and the like and completely has the potential of industrial production.

The invention relates to bacillus amyloliquefaciens and an application thereof, belonging to the technical field of microbial fermentation. The invention provides a new strain namely bacillus amyloliquefaciens, wherein the collection number of the strain is CGMCC No.9928, the collection date is November 4, 2014, and the collection unit is General Microbiological Center of China Committee for Culture Collection of Microorganisms (CGMCC). Bacillus amyloliquefaciens with the collection number of CGMCC No.9928 provided by the invention can be used for producing naringinase. According to bacillus amyloliquefaciens provided by the invention, bacillus amyloliquefaciens capable of producing naringinase is separated and cultured for the first time; and the naringinase activity in a liquid fermentation solution can reach 201.12 U/mL.

The invention relates to the technical field of biology and discloses a method for screening yield increase stimulants for rubber trees. The method comprises the steps of with stems or leaf stalks of budlings of the rubber trees in a bronze period as explants, disinfecting the explants, sectioning the explants, inoculating the explants to a reference culture medium and a screening culture medium added with to-be-detected chemical reagents, culturing under a dark condition of 24-28 DEGC for 60-100 days, calculating differentiation frequencies of laticiferous cells in callus tissues in the reference culture medium and the screening culture medium, and screening the to-be-detected chemical reagents which can be taken as the yield increase stimulants for the rubber trees according to a judgment standard that the differentiation frequency of the laticiferous cells in the callus tissues in the screening culture medium is remarkably higher than the differentiation frequency of the laticiferous cells in the callus tissues in the reference culture medium. Compared with exiting methods, the method is good in repeatability, is short in time, is not easily influenced by environments and can be applied to large-scale screening of the yield increase stimulants for the rubber trees, and the screening efficiency of the yield increase stimulants for the rubber trees can be greatly improved.

The invention relates to a method of preparing volvaria volvacea essential powder from volvaria volvacea deeply fermented mycelia. The method includes the steps of: 1) deeply fermenting the mycelia; 2) breaking the mycelia; 3) performing enzymolysis; 4) performing concentration; and 5) spray-drying a product to prepare the volvaria volvacea essential powder. In the method, the deep fermentation technology is combined with oriented bio-enzymolysis so as to prepare the volvaria volvacea essential powder with the deeply fermented mycelia as a raw material. The method, compared with a conventional method of deep processing of the volvaria volvacea with fruiting body, has important breakthrough. The volvaria volvacea essential powder is 80-90% in peptidyl nitrogen and is high in content (more than 85%) of small peptide (relative molecular mass being less than 1000D). The volvaria volvacea essential powder has a plump and mellow umami flavor.

The invention relates to a device and a method for culturing microalgae in a large scale, in particular to a cylinder-shaped thin film reactor device for fast culturing the microalgae. In the cylinder-shaped thin film reactor device, a main body is a vertical steel structure cylinder-shaped or polygonal cylinder bracket, the upper end and the lower end of the bracket are formed by a plurality of stainless steel metal straps arranged in a circular shape through welding, and the cylinder-shaped or polygonal cylinder bracket is arranged in a rectangular formation or a square formation. The lower end of the cylinder-shaped thin film reactor is opened, a pipe is arranged at the position of an opening, the pipe and a thin film are integrated to be a closed structure, the pipe is of a screw structure, the reactor is directly sleeved on the base of the cylinder-shaped bracket, and the upper end of the reactor is provided with an exhaust port, a feed port and an inlet port. A top cover of the reactor is installed and fixed at the upper end of the cylinder-shaped bracket so that direct impact of rainwater, and the like and attachment of dust can be avoided. The base of the cylinder-shaped bracket is provided with the opening, the opening is of the screw structure and can be connected with the pipe of the opening at the lower end of the thin film reactor, and raw material belts are filled in connection position of the opening and the pipe for closing.

The invention relates to a ZnO-base light emitting diode, which uses ZnO as base, to deposit a n-ZnO film layer, a n-Zn1-xMgxO film while 0<x<0.6, a ZnO quantum well layer, a p-Zn1-xMgxO film while 0<x<0.6, a p-ZnO film, and a second electrode on one face of a liner from down to up, and deposit a first electrode on another face of the liner. The ZnO quantum well layer is composed of z periodical ZnO/Zn1-yMgyO quantum well layers, while 0<y<0.5, and z value is 5-10.The invention uses metal organic chemical gas deposit method to grow product, wherein the p-ZnO and p-Zn1-xMgxO film use the non-plasma of O2/NO mixed gas to assist nitrogen doping. The invention optimizes the NO/O2 ratio to improve the nitrogen doping density, to improve the device property. The ZnO-base light emitting diode uses quantum well as active layer to improve lighting efficiency.

A method of preparing nano-metal sulfide by microorganism, it comprises the steps of: it adapts the photosynthetic bacterium as the inoculants, which is fixed and activated; we put metal salts into substrate, then antisepticises, inoculates the bacterium, incubated at room temperature, illumination, culture condition for 12-72 hours; after ageing, we remove the supernatant, fluid, wash and dry the product, and then obtains the nano-metal sulfide. The products particulate is quite small, distributing is symmetrical, and has a high purity. It is prone to control the reaction with no pollution in the process, and low cost, and is prone to industrialization.

The invention discloses a callus induction and subculture medium for cyclocarya paliurus, and also discloses a culture method based on the callus induction and subculture medium for the cyclocarya paliurus. The culture method includes explant selection, explant sterilization, establishment of a sterile anti-browning system, and proliferation culture. The callus induction and subculture medium andthe culture method have the advantages that the anti-browning abilities of calluses of the cyclocarya paliurus can be effectively improved, a large quantity of non-browning calluses with high growth speeds can be obtained in a short time, the problem of resource shortage of the cyclocarya paliurus can be solved through biotechnological measures, and accordingly, the foundation is laid for industrial extraction of secondary metabolites of the cyclocarya paliurus, such as triterpenes, flavonoids and polysaccharides, in later period.

The invention discloses a method for constructing a composite material based on bean sprouts, and particularly relates to the field of supercapacitors. A metal salt solution is utilized to culture bean sprouts, so that living bean sprouts absorb metal ions, and after carbonization and activation, the composite electrode material of activated carbon and metal oxide can be prepared. Soybeans are irrigated and cultivated with a metal salt solution, living bean sprouts containing metal salt are cultivated by controlling the variety and concentration of a metal ion solution, living organisms contain metal ions, and then an electrode material of an activated carbon/metal oxide compound is directly prepared through carbonization and activation; bean sprouts which are low in price and extremely easy to meet the growth environment are adopted, and living biomass containing metal salt is obtained through a simple metal ion solution irrigation cultivation method, so that the supercapacitor electrode material is prepared.