Method for screening yield increase stimulants for rubber trees

Abstract

The invention relates to the technical field of biology and discloses a method for screening yield increase stimulants for rubber trees. The method comprises the steps of with stems or leaf stalks of budlings of the rubber trees in a bronze period as explants, disinfecting the explants, sectioning the explants, inoculating the explants to a reference culture medium and a screening culture medium added with to-be-detected chemical reagents, culturing under a dark condition of 24-28 DEGC for 60-100 days, calculating differentiation frequencies of laticiferous cells in callus tissues in the reference culture medium and the screening culture medium, and screening the to-be-detected chemical reagents which can be taken as the yield increase stimulants for the rubber trees according to a judgment standard that the differentiation frequency of the laticiferous cells in the callus tissues in the screening culture medium is remarkably higher than the differentiation frequency of the laticiferous cells in the callus tissues in the reference culture medium. Compared with exiting methods, the method is good in repeatability, is short in time, is not easily influenced by environments and can be applied to large-scale screening of the yield increase stimulants for the rubber trees, and the screening efficiency of the yield increase stimulants for the rubber trees can be greatly improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for screening rubber tree yield-increasing stimulants. Background technique [0002] Rubber tree (Hevea brasiliensis Muell-Arg) belongs to Euphorbiaceae and is an important tropical economic crop. The bark of the rubber tree is cut regularly, and the latex (cytoplasm) is discharged from the cut and processed into natural rubber. At present, the natural rubber produced by the rubber tree accounts for more than 99% of the world's total natural rubber output. It is listed as the four major industrial raw materials together with petroleum, coal, and iron ore, and is an important strategic material. [0003] The application of chemical agent control technology to promote the rubber production and rubber removal technology of rubber trees is an important technology to improve the productivity of rubber trees, and it is also an important content of rubber production and theoretica...

AI Technical Summary

Problems solved by technology

This method has the following significant disadvantages: ① It occupies a large area and the test cost is high

When verifying the effect of chemical agents on increasing the production of rubber trees, a large area of ​​mature forest sections is required, which consumes a lot of manpower and material resources, resulting in high test costs

②Some chemicals may have harmful effects on the gum tree, which will cause the economic value of the gum tree to lose; ③Environmental factors are difficult to control

The test is carried out in the field, which is easily affected by environmental factors such as seasons, climates, differences between plants, etc., often resulting in poor repeatability of the test

④Long test period

These shortcomings limit the large-scale screening of rubber tree chemicals

So far, only a few chemical reagents such as jasmonic acid and ethephon have been tested on the effect of tapping rubber production, but the production increasing effect of a large number of other chemical agents has not been verified due to the limited test conditions, which restricts the screening of rubber tree production increasing stimulants. efficiency

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