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A medium for callus induction and sub-proliferation of astragalus plants

A technology of callus induction and subculture, which is applied to plant cells, plant regeneration, botany equipment and methods, etc., can solve the problems of shortage of Astragalus membranaceus plant resources, increase photosynthesis, reduce the amount of agar used, and shorten the production cycle Effect

Active Publication Date: 2018-01-09
甘肃省农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a medium formula for induction and subculture propagation of Astragalus plant callus and a culture method thereof, which can obtain a large amount of Astragalus leaf callus without browning in a relatively short period of time, through biotechnology Means to solve the problem of lack of Astragalus plant resources, and provide sufficient raw materials for the industrial extraction of active ingredients of Astragalus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A. Selection and sterilization of explants: Astragalus leaves were used as explants. Collect healthy and disease-free Astragalus leaves, wash them 5 times with sterile water, soak them in 75% ethanol for 30 seconds, and then put them in 0.10% HgCl 2 Soak in the solution for 12.0 minutes, then wash 3 times with sterile water.

[0047] B. Sterilization of the culture medium: the callus induction medium and the subculture proliferation medium are respectively packed in 100ml triangular culture bottles, 20.0ml per bottle, and the bottle mouth is covered with air-permeable plastic sealing film, and sterilized at a pressure of 0.10MPa 20.0 minutes, cool for later use.

[0048] C. Callus induction: take the sterilized leaves of Astragalus membranaceus and cut them into 2.0 mm long sections with a sterile scalpel, and inoculate them on the sterilized callus induction medium. The formula of callus induction medium is: MS+6-BA1.5mg / L+NAA0.8mg / L+sucrose 20.0g / L+glucose 10.0g / L+a...

Embodiment 2

[0051] A. Selection and sterilization of explants: Astragalus leaves were used as explants. Collect healthy and disease-free Astragalus leaves, wash them three times with sterile water, soak them in ethanol with a volume concentration of 72% for 40 seconds, and then put them in HgCl with a mass concentration of 0.12%. 2 Soak in the solution for 8.0 minutes, then wash 4 times with sterile water.

[0052] B. Sterilization of the culture medium: the callus induction medium and the subculture proliferation medium are respectively packed in 100ml triangular culture bottles, each bottle is 22.0ml, the bottle mouth is covered with a breathable plastic sealing film, and sterilized at a pressure of 0.15MPa 20.0 minutes, cool for later use.

[0053] C. Callus induction: take the sterilized leaves of Astragalus membranaceus and cut them into 4.0 mm long sections with a sterile scalpel, and inoculate them on the sterilized callus induction medium. The formula of callus induction medium ...

Embodiment 3

[0056] A. Selection and sterilization of explants: Astragalus leaves were used as explants. Collect healthy and disease-free Astragalus leaves, wash them three times with sterile water, soak them in 70% ethanol for 50 seconds, and then put them in 0.15% HgCl 2 Soak in the solution for 5.0 minutes, then wash 5 times with sterile water.

[0057] B. Sterilization of the culture medium: the callus induction medium and the subculture proliferation medium are respectively packed in 100ml triangular culture bottles, each bottle is 25.0ml, the bottle mouth is covered with a breathable plastic sealing film, and sterilized at a pressure of 0.12MPa 20.0 minutes, cool for later use.

[0058] C. Callus induction: take the sterilized leaves of Astragalus membranaceus and cut them into 5.0 mm long sections with a sterile scalpel, and inoculate them on the sterilized callus induction medium. The formula of callus induction medium is: MS+6-BA1.0mg / L+NAA0.6mg / L+sucrose 18.0g / L+glucose 8.0g / L+...

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Abstract

The invention relates to a medium formula for induction and subculture proliferation of astragalus plant callus and a culture method thereof. Including explant selection and sterilization, medium sterilization, callus induction, callus subculture four steps. Astragalus plant callus culture method uses Astragalus leaves as explants, and adds maltose, glucose, sucrose, agar powder, sodium alginate, naphthalene acetic acid, 6-benzylpurine, etc. Tissue culture to obtain the callus of leaves of Astragalus membranaceus. Adjust the pH value of the medium, and the prepared callus induction medium and callus subculture medium are respectively packed in triangular culture bottles, and the bottles are covered with air-permeable plastic sealing film, and sterilized at a pressure of 0.10~0.15MPa Then callus culture was carried out. The invention can realize effective induction and rapid proliferation of astragalus leaf callus, obtain a large amount of astragalus leaf callus in a relatively short period of time, has the characteristics of short production cycle and fast proliferation speed, and is suitable for industrial production.

Description

technical field [0001] The invention relates to a plant callus culture method, in particular to a medium for induction and subculture proliferation of astragalus plant callus. Background technique [0002] Astragalus (Astragalus sembranaceus (Fisch.) Bunge) is Fabaceae (Leguminosa esp.) Astragalus (AstragalusLinn.) plant, Astragalus contains saponins, sucrose, polysaccharides, various amino acids, folic acid, selenium, zinc, copper and other trace elements. Astragalus membranaceus has a high medicinal value. Raw astragalus membranaceus is beneficial for strengthening qi, diuresis, detumescence, detoxification, and muscle growth. It is suitable for spontaneous sweating, night sweats, blood numbness, edema, carbuncle and gangrene without ulceration or long-term ulceration. embolism. Honey-roasted Astragalus has the functions of invigorating qi, nourishing blood, and benefiting the middle. It is suitable for internal injuries, fatigue, spleen deficiency and diarrhea, qi def...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 赵瑛罗俊杰王红梅张运晖崔明九叶春雷欧巧明薛莲
Owner 甘肃省农业科学院生物技术研究所
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