Chaetomium globosum and application theroef

A technology of Chaetomium globosum and original bacteria, which is applied in the application, fungicides, fungi and other directions, can solve the problem of not involving the endophytic Chaetomium globosum isolated from endangered plants Coral vegetables, and bagging of young fruits to increase the economy and labor of fruit farmers. Input, not involving the control effect of Chaetomium globosum, etc., to achieve the effect of high yield, easy control of growth conditions, and strong inhibitory effect

Active Publication Date: 2015-09-02
黑龙江瑟高生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bagging technology has been widely used in apple field production. The problem is that bagging young fruit during the growth period increases the economic and labor input of the fruit grower. If the bagging time and technology are not mastered, it may cause even more unnecessary economic loss
[0004] Biological control has broad application prospects, because it has less pollution to the environment, no residue, and is safe for humans and animals. It is a hot topic in recent years. Patent: CN103194490A, a yellow basket-shaped fungus and its application in the prevention and treatment of plant pathogenic bacteria, Chinese patent: CN102286383A, but the above-mentioned documents do not involve the control effect of Chaetomium globosa
The literature on Chaetomium globosa involves its biological control effect, such as the preparation method of the anti-phytopathogenic fermentation liquid of endophytic fungus camptophytes, Chinese patent: CN103255065A, but none of them involve the isolation of endophytic Chaetomium globosa from endangered plants report

Method used

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  • Chaetomium globosum and application theroef
  • Chaetomium globosum and application theroef
  • Chaetomium globosum and application theroef

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Screening and antibacterial activity of endophytic fungi:

[0019] The screening medium used (PDA medium with antibiotics): 200g of potatoes, peeled and cut into pieces, steamed for 20min, then passed through 4 layers of gauze to collect the filtrate, added 20g of glucose, 20g of agar, heated and melted, and the volume was adjusted to 1000ml, followed by adding penicillin and streptomycin to a final concentration of 80ug / ml, the pH value was natural, 121°C, autoclaved for 20min, and poured onto a plate for later use.

[0020] Screening of endophytic fungi: Take fresh leaves from healthy adult coral dishes, rinse them under running water, and sterilize the surface of the materials, first treat them with 75% ethanol for 90S, then soak them in mercuric chloride for 5 minutes, and then soak them in sterile water. After washing for 4-5 times, cut the material into small pieces of 0.5cm*0.5cm on a sterile petri dish. Place the cut pieces of coral leaf tissue on...

Embodiment 2

[0027] Example 2: Identification of the genus of the endophytic fungus HY1:

[0028] Morphological characteristics: The colony was light yellow at the initial culture, growing in a round shape, with no obvious decoration on the surface of the colony, the colony was loose and flat, the hyphae were well developed and long, and the growth rate was fast (see attached figure 1 ); later, the color began to turn yellow and black, and the hyphae were spherical in liquid culture. Microscopic morphology observation revealed that the hyphae had a transverse septum shape, and the hyphae in the base radiated to the periphery (see appendix figure 2 ), the sporangia are surrounded by opaque black ascus shells, and the ascus shells have special hair-like structures.

[0029] 18S rDNA sequence analysis of endophytic strains: Genomic DNA of endophytic fungus HY1 strain was extracted by CTAB method, using 18Sr DNA universal primers (NS: 5'-GTAGTCTATGCTTGTCTC-3' and NS6: 5'-GCATCACAGCCTGTTATTG...

Embodiment 3

[0063] Embodiment 3: antibacterial activity of chaetomium globosa HY1 intracellular and extracellular metabolites

[0064] Filter the culture medium of the strain HY1 cultured in liquid with 8 layers of sterile gauze, collect the fermentation broth and mycelia respectively, and the fermentation broth is the extracellular metabolite liquid. After the mycelium was dried at 60°C, it was ground into powder, extracted with distilled water at a ratio of 1:10 (g / ml) for 18 hours, and then crushed with an ultrasonic cell breaker (5min, 600W) to further extract its chemical components to obtain intracellular metabolite liquid. With apple anthracnose bacteria as indicator bacteria, add 0.05ml, 0.1ml, 0.15ml, 0.2ml, 0.25ml, 0.5ml, 0.75ml, 1.0ml, 1.25ml of extracellular metabolite solution per 100ml of culture medium; Product liquid 0.25ml, 0.5ml, 0.75ml, 1.0ml, 1.25ml, carries out the mensuration of antibacterial activity, and assay method is the same as the assay of the activity of inh...

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Abstract

The invention discloses chaetomium globosum with strong inhibition effect agaisnt anthracnose pathogens of apples. The chaetomium globosum is registered and booked with the number being CGMCC No. 10609 in general microorganism center of China committee for culture collection of microorganisms, and the collection date is march 11th, 2015. The chaetomium globosum HY1 disclosed by the invention is endophytic fungi of glehnia littoralis as a threatened plant, both fermentation liquor and mycelium extracting solution of the chaetomium globosum have strong inhibition effect against the anthracnose pathogens of the apples, and also have certain inhibition action against other plant pathogens, and the strain has wide application prospect in the aspects of preventing and treating the anthracnose pathogens of the apples and the like.

Description

Technical field: [0001] The invention relates to a strain of Chaetomium globosa which has a strong inhibitory effect on the pathogenic bacteria of apple anthracnose, and belongs to the field of biotechnology. Background technique: [0002] Apple anthracnose, also known as bitter rot, is one of the main diseases of apple fruit. It occurs in most apple producing areas in my country, especially in areas with high temperature, rain and humidity in summer. The fruit can be infected after fruit setting, but the disease begins when the fruit is close to maturity, and continues to develop during the storage period after harvest, resulting in a large number of fruit drop before harvest and fruit rot in postharvest storage. [0003] In my country, apple anthracnose is mainly caused by G. anthracnose, and the control agents are mainly aimed at this pathogen. At present, the methods of preharvest control of apple anthracnose mainly include: bagging during growth period, chemical contro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00
CPCA01N63/30C12N1/145C12R2001/645
Inventor 张萍毛志远程仕伟
Owner 黑龙江瑟高生物科技有限公司
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