33 results about "Chaetomium sp." patented technology

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention discloses a microbial compound inoculum for the production of cellulosic ethanol and a preparation method thereof, relating to a compound inoculum and a preparation method thereof, and solving the problems that the cost of fermentation production of cellulosic ethanol is high due to the adoption of a rinsing and detoxification method, and the ultimate yield of the ethanol is low because the rinsing and detoxification method loses the nutrition elements in the lignocellulose. The microbial compound inoculum is mainly prepared from a trichoderma koningii spore suspension, an aspergillus oryzae spore suspension and a chaetomium cupreum spore suspension. The preparation method comprises the following steps: firstly, mixing the trichoderma koningii spore suspension, the aspergillus oryzae spore suspension and the chaetomium cupreum spore suspension; and secondly, mixing and inoculating the mixed spore suspension in a culture medium for cultivation to obtain the microbial compound inoculum. The microbial compound bacterium has few dosage and reduces the cost of the fermentation production of cellulosic ethanol, and the ethanol prepared from the microbial compound inoculum has high ultimate yield.

The invention provides an application of Chaetomium globosa in preventing and treating wheat stem base rot. The preservation name of the Chaetomium globosa is 12XP1-2-3, the preservation number is CGMCC No.17183, and the preservation unit is China Microbial Bacteria The General Microbiology Center of the Species Depository Management Committee, the deposit date is January 23, 2019. The field test results showed that using Chaetomium globulus 12XP1‑2‑3 to coat wheat seeds could reduce the diseased stem rate, disease grade and disease index by 19.0%‑41.3%, 43.7% and 4.7%‑45.4%, respectively.

The invention discloses a preparation method and application of a secondary metabolite of the endophytic fungus Chaetomium sp., the method comprising: (1) activating and subculturing the endophytic fungus Chaetomium sp. on PDA solid medium, Then shake culture in PDB liquid medium; (2) ferment and cultivate the liquid seeds obtained in step (1) in rice medium to obtain solid fermented product; (3) solid fermented product is extracted by methanol cold immersion, filtered, The filtrate is concentrated to obtain a crude extract, and the crude extract is suspended in water and extracted with petroleum ether and ethyl acetate to obtain the crude extract of petroleum ether and ethyl acetate layer respectively; (4) by vacuum column chromatography, dextran Compound A‑F was obtained by gel column chromatography and semi-preparative high performance liquid chromatography. The method of the invention separates the secondary metabolites of the endophytic fungus Chaetomium sp., and the secondary metabolites have antibacterial and antitumor effects.

The invention discloses an endophytic fungus Chaetomium sp. HQ-1 with good antibacterial activity. The astragalus endophyte HQ‑1 active product differanisole A to G + Bacteria Staphylococcus aureus S.aureus, methicillin-resistant Staphylococcus aureus MRSA, Listeria monocytogenes L.monocytogenes showed moderate inhibitory activity. It showed a strong inhibitory effect on the pathogenic fungus S. rolfsii, indicating that differanisole A has the potential as an agricultural antibiotic. The astragalus endophytic fungus Chaetomium sp.HQ‑1 provided by the invention can provide new resources and approaches for the production of antibacterial drugs, especially for the production of agricultural antibiotics against plant pathogenic fungi.

The invention belongs to the technical field of biology and particularly relates to a method for extracting oosporein from a chaetomium cupreumfermentation liquor. The method comprises the steps thatchaetomium cupreum is obtained through screening and separation and is fermented, filtered, concentrated, acidized, extracted, re-concentrated and crystallized, and the oosporein is finally obtained.The oosporein directly acts on plant diseases, the limitations of the chaetomium cupreum serving as a biocontrol bacterium for biological prevention and control are overcome, use of medication is accurate, plant damage is reduced, the dosage of an organic solvent for extracting the oosporein by using a direct extraction method is decreased, the energy consumption needed by oosporein extraction isreduced, the extracting efficiency is improved, the effect of conveniently and quickly obtaining high-purity oosporein at low energy consumption is achieved, and the method has a great popularizationand use value.

The invention discloses a method for fermenting and producing chitinase by Chaetomium globosa and its application. The strain is activated by using Chaetomium globosa HY1, and the activation medium includes glucose, peptone, potassium dihydrogen phosphate, dihydrogen phosphate Sodium; then shaker culture or fermenter culture, the fermentation medium includes soluble starch, glycerol, tryptone, yeast powder, corn steep liquor, NH 4 NO 3 , MnSO 4 , MgSO 4 、Na 2 HPO 4 、K 2 HPO 4 , Colloidal chitin, the chitinase produced by fermentation is used for the preparation of chitooligosaccharides. The cultivation method of the invention is simple, the nutritional requirements are not high, the chitinase produced by liquid fermentation has high activity, and has great industrial application value.

The invention relates to the field of genetic engineering, in particular to a high temperature resistant chaetomium polygalacturonase mutant and an encoding gene and an application thereof. Polygalacturonase from achaetomium sp.xz8 is used as a female parent, and the polygalacturonase mutant with improved heat stability is obtained through rational design and molecular biological technique. Related amino acid mutation is combined mutation (ASA, Asp244Ala / Asp299Arg) of two points Asp244Ala and Asp299Arg in each mutant sequence. Optimum temperature, T50 and T1 / 2 of the mutant are improved in comparison with those of wild type enzyme. The optimum temperature is increased from 45 DEG C to 55 DEG C, T50 is increased from 56 DEG C to 73 DEG C, half-life period T1 / 2 at 50 DEG C is increased from 1.5h to 10h, half-life period T1 / 2 at 55 DEG C is increased from 33min to 78min, and Tm value is increased from 43.8 DEG C to 54.1 DEG C. Besides, the mutant keeps enzyme catalytic activity which is equal to that of the wild type enzyme. Application demands in fields of feedstuff, foodstuff and the like can be met.

The invention relates to a preparation method of an antibacterial fermentation liquid of Solidago canadesis endophytic fungi, which comprises the following operation steps: 1. activating strain; 2. carrying out first-stage seed culture; 3. carrying out second-stage seed culture; and 4. carrying out fermentation culture to obtain brown Solidago canadesis endophytic fungus fermentation liquid, filtering the Solidago canadesis endophytic fungus fermentation liquid in aseptic condition to remove mycelia, and then passing a 0.22 mu m filter to obtain the antibacterial fermentation liquid of Solidago canadesis endophytic fungi. According to the invention, the preparation method of antibacterial fermentation liquid of Chaetomium globosum yt03 with broad spectrum antibacterial activity is determined; and antibacterial test finds that the Chaetomium globosum yt03 antibacterial fermentation liquid has relatively significant hypha growth inhibiting action on six tested plant pathogenic fungi.

The invention provides a preparation method and application of a biological seedling raising substrate of chaetomium globosum, and belongs to the technical field of agricultural microorganisms. The method comprises the steps that after preliminary fermentation is performed on agricultural waste, the agricultural waste is inoculated with chaetomium globosum ND35 sexual conidial powder to perform secondary fermentation, so that a functional flora taking chaetomium globosum as the main microorganisms in the substrate, and the biological seedling raising substrate of chaetomium globosum is obtained. The agricultural waste comprises, but is not limited to, bark, wood chips, straw and the like. The preparation method and application of the biological seedling raising substrate of chaetomium globosum have the advantages of being simple, being capable of being directly applied to the seedling raising stages of tomatoes, cucumbers and other plants, planting chaetomium globosum ND35 bacterial strains into the plants more directly and quickly, and being beneficial to the plants perpetually; the biological seedling raising substrate of chaetomium globosum is an excellent seedling raising substrate, can be applied to industrialized seedling raising of various plants, fills in the blank of domestic excellent substrates, replaces imported substrates, promotes the healthy development of industrialized seedling raising of vegetables, and has great economic and social benefits.

The invention provides an application of Chaetomium globosa in the prevention and treatment of wheat take-all. The preservation name of the Chaetomium globosa is 12XP1-2-3, the preservation number is CGMCC No.17183, and the preservation unit is China Microorganisms The General Microorganism Center of the Preservation Management Committee, the deposit date is January 23, 2019. The Chaetomium globosa 12XP1‑2‑3 has an inhibitory rate of 45.4% on the flat plate to the pathogen GaNS90 of wheat take-all. Reduce white ear rate and disease index of wheat.