33 results about "Chaetomium sp." patented technology

The invention discloses a chaetomium globosum FSR-74. The collection unit of the chaetomium globosum FSR-74 is China General Microbiological Culture Collection Center, the collection date of the chaetomium globosum FSR-74 is September 19, 2014 and the collection number of the chaetomium globosum FSR-74 is CGMCC No. 9690. The strain is relatively slow in growth and has light brown colonies and an irregular wave-like edge when subjected to plate culture at 25 DEG C in a potato dextrose agar (PDA) culture medium. FSR-74 ascocarps are supergene and fixed on the surface of a matrix by rhizoids; top hair is wavy, brown and has intervals, and dense wart points are arranged on the surface; 8 ascospores are arranged in an ascus; the ascospores are shaped like lemons and have single apical bud holes. The chaetomium globosum FSR-74 has an efficient broad-spectrum bacteriostatic effect against main pathogenic bacteria causing ginseng rust rot, blight, sclerotinia rot, rust rot, blackspot, sheath blight and gray mold. The invention further provides a biological control mechanism and application of the chaetomium globosum FSR-74 in prevention and treatment of plant fungal diseases, as well as application in preparation of a microbial agent for preventing and treating the plant fungal diseases.

The invention discloses a method for manually inducing Aquilaria sinensis to generate agilawood. The method comprises the following steps of: injecting a methanoic acid or acetic acid solution with pH value of 1.5-3 or a mixed solution of methanoic acid and acetic acid into xylem of Aquilaria sinensis [Aquilaria sinensis (Lour.) Gilg]; respectively injecting the solution together with fungi, suchas Botryosphaeria rhodina, Hypocrea jecorina, Fusarium sp., Colletotrichum gloeosporioides, Ampelomyces sp, Khuskia sp, Fungal endophyte sp.A 16, Pestalotiopsis sp., Hypocrea lixii and Chaetomium sp.or injecting the solution and the fungi in a mixed way; and inducing the Aquilaria sinensis to generate the agilawood. According to the invention, the methanoic acid or acetic acid solution or the mixed solution of methanoic acid and acetic acid is injected into the xylem of the Aquilaria sinensis to induce the Aquilaria sinensis to generate defense reaction so as to generate the agilawood; and alarge amount of agilawood are generated by using the method. The method for manually inducing the Aquilaria sinensis to generate the agilawood, disclosed by the invention, has the advantages of simplicity and convenience in operation, low cost and suitability for manually making agilawood on a large scale by using Aquilaria sinensis trees; and a large amount of agilawood can be effectively generated by the Aquilaria sinensis. According to the invention, the agilawood producing period of the Aquilaria sinensis is largely reduced, the agilawood can be generally obtained after 6-24 months, the production cost is reduced, the market requirements are satisfied, and important economic, social and ecological benefits are obtained in aspects of protection and sustainable utilization of the Aquilaria sinensis, economic development of mountainous regions and the like.

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.

The invention relates to a preparation method of an antibacterial fermentation liquid of Solidago canadesis endophytic fungi, which comprises the following operation steps: 1. activating strain; 2. carrying out first-stage seed culture; 3. carrying out second-stage seed culture; and 4. carrying out fermentation culture to obtain brown Solidago canadesis endophytic fungus fermentation liquid, filtering the Solidago canadesis endophytic fungus fermentation liquid in aseptic condition to remove mycelia, and then passing a 0.22 mu m filter to obtain the antibacterial fermentation liquid of Solidago canadesis endophytic fungi. According to the invention, the preparation method of antibacterial fermentation liquid of Chaetomium globosum yt03 with broad spectrum antibacterial activity is determined; and antibacterial test finds that the Chaetomium globosum yt03 antibacterial fermentation liquid has relatively significant hypha growth inhibiting action on six tested plant pathogenic fungi.

The invention provides chaetomium globosum (Chaetomium globosum), which has a preservation name of 12XP1-2-3 and a preservation number of CGMCC No.17183, wherein the preservation unit is the China general microbiological culture collection centre; and the preservation date is January 23th, 2019. The invention further provides application of chaetomium globosum in promoting growth and increasing yield of wheat. Field test results show that: when the chaetomium globosum 12XP1-2-3 provided by the invention is used for wheat seed coating treatment, the chlorophyll content of leaves can be increased by 1.9%-14.7% in the booting stage; and the wheat yield can be increased by 2.3%-10.9%.

The invention discloses chaetomium globosum, a fungicide, a seed soaking solution and an application thereof, and relates to the technical field of agricultural biology. The strain is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 61363, and the taxonomic name of the strain is Chaetomium globosum. Experiments prove that the strain has broad spectrum for improving the stress resistance of crops, and can obviously improve the drought resistance and saline-alkali resistance of the crops. Under the stress of a drought environment, the chaetomium globosum MN110495 can generate substances such as exopolysaccharides and antioxidants to resist the drought environment, so that the drought resistance of various plants such as fruits, vegetables and food crops is improved. The chaetomium globosum also has the effects of promoting seed germination and crop growth.

The invention discloses a method for producing chitinase through fermentation of chaetomium globosum and application of the chitinase. According to the method, chaetomium globosum HY1 is adopted for strain activation, an activation culture medium comprises glucose, peptone, monopotassium phosphate and disodium hydrogen phosphate, then shake cultivation or fermentation tank cultivation are carriedout, a fermentation medium comprises soluble starch, glycerin, tryptone, yeast powder, corn steep liquor, NH4NO3, MnSO4, MgSO4, Na2HPO4, K2HPO4 and colloidal chitin, and chitinase produced through fermentation is used for preparing chitosan oligosaccharide. The culture method is simple, the nutritional requirement is not high, and the chitinase produced by liquid state fermentation is high in activity and has a relatively great industrial application value.

The invention belongs to the technical field of biological detection, and discloses a primer for detecting chaetomium globosum in jet fuel, application and a detection method. The primer for detectingthe chaetomium globosum comprises an outer primer F3, an outer primer B3, an inner primer FIP, an inner primer BIP and a loop primer LB; and the sequence of the outer primer F3 is shown as SEQ ID NO.1, the sequence of the outer primer B3 is shown as SEQ ID NO.2, the sequence of the inner primer FIP is shown as SEQ ID NO.3, the sequence of the inner primer BIP is shown as SEQ ID NO.4, and the sequence of the loop primer LB is shown as SEQ ID NO. 5. The detection method has the advantages of being high in sensitivity and specificity.

The invention discloses chaetomium globosum, which is a chaetomium globosum (Chaetomiumglobosum) 12CG5-11 strain, is preserved in China General Microbiological Culture Collection Center on August 4, 2020, has a preservation number of CGMCC No.19939, has a preservation address of No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, and is named as chaetomium globosum (Chaetomiumglobosum) in taxonomy. The chaetomium globosum spore liquid is produced through fermentation of a millet grain culture medium, the chaetomium globosum spore liquid is applied to preparation of a medicament for preventing and treating wheat sharp eyespot, and field test results show that after the chaetomium globosum 12CG5-11 spore liquid is sprayed in the sowing period of wheat, the prevention effects on the wheat sharp eyespot in the jointing period and the mature period reach 47.83% and 53.40% respectively.