23 results about "Chaetomium sp." patented technology

The present application belongs to the field of biotechnology, and particularly relates to a preparation method and application of five compounds (1-5) extracted from liquid fermentation of Chaetomium sp. Specifically, the present application discloses five compounds, wherein compound 1 is a new compound. The Chaetomium sp. metabolite has good antibacterial activity on three tested pathogenic bacteria (Pseudomonas syringae pv. actinidiae, Xanthomonas oryzae pv. oryzae and Xanthomonas oryzae pv. oryzicola), and has important significance for development of a new type of microbial source antibacterial agent.

The present application provides a new Chaetomium sp. strain, which is preserved in China General Microbiological Culture Collection Center (CGMCC) with a preservation number of CGMCC NO.23896; the present application also provides a new compound shown as formula I produced by solid fermentation of the strain, wherein the compound has stable performance, no cytotoxicity, and significant neuroprotective activity; the compound can be obtained by ethyl acetate extraction on the fermentation product of the aforementioned strain, followed by silica gel column chromatography and high performance liquid chromatography separation and purification, and the separation steps are simple, easy to implement, easy to mass produce in industry, and have broad application prospects.

The present invention belongs to the field of microbial applications, specifically relating to a Chaetomium globosum DX-THS3 bacterial fertilizer, its preparation method, and its application. The present invention finds that Chaetomium globosum DX-THS3 significantly promotes the growth of peppers. Furthermore, it not only significantly inhibits peach brown rot and pythium in the soil, but also improves the soil itself. This significantly broadens the application scope of Chaetomium globosum and provides a new technical means for soil regulation and pepper growth promotion.

The invention provides a recombinant mutant dextranase from chaetomium graciliforme as well as a construction method and application thereof, and belongs to the technical field of mutant enzymes. The recombinant mutant dextranase provided by the invention is CgDEXA289V, and the amino acid sequence of the recombinant mutant dextranase is as shown in SEQ ID NO: 21. Non-conservative site mutation is carried out on the basis of a Chaetomium gracile-sourced dextranase coding gene, a high-enzyme-activity mutant is preliminarily screened out through escherichia coli and is expressed in a pichia pastoris expression system, the high-performance mutant enzyme CgDEXA289V is obtained, the enzyme is endo-dextranase and has good substrate affinity for a substrate, and the mutant enzyme CgDEXA289V can be used for preparing a high-performance mutant enzyme CgDEXA289V. Due to higher enzymatic reaction rate and better stability, the enzymolysis preparation efficiency can be improved, the production cost is reduced, and the method has a better application prospect.

This invention belongs to the field of saline-alkali land improvement technology, specifically relating to a compound microbial agent and its application in saline-alkali land improvement. This invention prepares a compound microbial agent for improving saline-alkali land using microorganisms such as Chaetomium coccidioides, Bacillus subtilis, Bacillus licheniformis, Bacillus belye, and humic acid. When applied to saline-alkali land improvement, this agent not only significantly improves the soil's physical and chemical properties but also significantly reduces the number of stunted cotton seedlings, increases cotton seed yield, and improves fiber quality.

The invention belongs to the technical field of biocontrol microorganisms, and particularly relates to chaetomium globosum 5141-4, a fungicide and application of the chaetomium globosum 5141-4 and the fungicide. According to the invention, a chaetomium endophytic fungus is separated from the ground coat of litmus, and the chaetomium endophytic fungus is named as chaetomium globosum 5141-4. Through verification, the strain can effectively inhibit growth of fusarium oxysporum or colletotrichum gloeosporioides, has a good inhibition effect on six pathogenic bacteria including pseudomonas aeruginosa and enterococcus faecalis, and is expected to be used for preparing biocontrol products or further screening antibacterial active compounds.

The invention belongs to the technical field of microbial agents, and relates to chaetomium globosum NtCg1 and application thereof in embedding biocontrol bacterium microbial microcapsules. The chaetomium globosum NtCg1 has an obvious antagonistic effect on various root rot pathogenic bacteria, the control effect on mung bean root rot reaches 62.16%, the control effect on tobacco root rot reaches 59.62%, and the root rot phenomenon caused by infection of the root rot is effectively restrained. According to the invention, the chaetomium globosum NtCg1 and the acremonium corioli NtSi1 are adopted to prepare the sodium alginate embedded biocontrol bacterium microorganism microcapsule, the control stability is improved through the synergistic effect of strains, and a better root rot control effect is achieved.

The invention discloses application of chaetomium to preparation of medicines, health care products and cosmetics for resisting skin injury. Through skin injury resisting activity analysis of the chaetomium cuspidatum, it is verified that the chaetomium cuspidatum can have a good skin injury resisting effect, and when the chaetomium cuspidatum is applied to medicine, health care products and cosmetics, the skin injury can be reduced, and the repairing effect is achieved.

The invention discloses chaetomium formosanum and application thereof in prevention and treatment of pine wood nematode disease, and belongs to the technical field of biocontrol of plant diseases. The name of the bacterial strain is Chaetomium ascotrichioides 1-24-2, and the preservation number of the bacterial strain is GDMCC No: 64397. The invention further discloses a preparation method of the chaetomium ascotrichioides strain. The chaetomium formosanum 1-24-2 disclosed by the invention is derived from resistant masson pine plant tissues; the chaetomium formosanum 1-24-2 and the biological agent obtained based on the chaetomium formosanum 1-24-2 have a strong inhibition effect on pine wood nematode diseases, are low in requirements on culture conditions and have good development and application prospects.

This invention discloses a recombinant β-glucanase bacterium, its construction, and its application in the preparation of water-soluble β-glucan. Belonging to the fields of genetic engineering and enzyme engineering, the recombinant plasmid pWB980-g9050 is introduced and expressed in the β-glucanase recombinant bacterium. The plasmid contains the gene sequence shown in SEQ ID NO.1 from *Chaetoceros* JY25 with accession number CGMCC No. 6882. The full gene sequence of the recombinant plasmid is shown in SEQ ID NO.2. This recombinant bacterium can be used in the preparation of water-soluble β-glucan. This invention achieves efficient and targeted biotransformation of yeast β-glucan by constructing a recombinant *Bacillus subtilis* strain that efficiently expresses a specific endoglucanase and employing a simple process based on crude enzyme solution fermentation. This not only stably produces water-soluble products with concentrated molecular weight distribution and well-defined structures, but also utilizes mild and environmentally friendly processes, demonstrating excellent potential for industrial application.

The invention belongs to the technical field of application of chaetomium globosum, and particularly relates to chaetomium globosum Ba08, a solid-state fermentation biological agent and application of the chaetomium globosum Ba08, and the preservation number of the chaetomium globosum Ba08 is CCTCC M 2025292; the solid state fermentation biological agent comprises chaetomium globosum Ba08 or metabolite; the invention discloses application of a solid-state fermentation biological agent in in-situ remediation of organic contaminated soil. The organic contaminated soil comprises polycyclic aromatic hydrocarbon contaminated soil, chlorine-containing organic contaminated soil and petroleum contaminated soil. The chaetomium globosum Ba08 can secrete cytochrome enzyme P450, manganese peroxidase and laccase under the aerobic condition, and C-C conjugated double bonds of aromatic hydrocarbon are broken through enzyme action, so that physiological toxicity of polycyclic aromatic hydrocarbon metabolism intermediate products is relieved, benzene series, heterocyclic compounds and polycyclic aromatic hydrocarbon can be thoroughly mineralized, and the yield of the product is increased. And the utilization rate of the petroleum biodegradation residues is obviously improved.

This invention belongs to the field of biocontrol microbial technology, specifically relating to a strain of Chaetomium globosum (… Chaetomium globosum )5141-4 、 Inoculants and their applications. This invention isolates an endophytic fungus from *Lithocarpus* lichens and names it *Chaetoceros globosa*. Chaetomium globosum 5141-4. Verification has shown that the above strains can effectively inhibit the growth of Fusarium oxysporum or anthrax, and also exhibit good inhibitory effects against six pathogens, including Pseudomonas aeruginosa and Enterococcus faecalis. They hold promise for use in the preparation of biocontrol products or further screening of antibacterial compounds.

The invention relates to the technical field of microorganisms, in particular to a culture method of chaetomium globosum strain H-1 with high yield of uranidin, which comprises the following steps: (1) activating the strain; (2) preparing an optimized culture medium which comprises 20-30 g / L of glucose, 5-9 g / L of fish peptone, 0.1-0.5 g / L of dipotassium phosphate and the balance of deionized water, and adjusting the initial pH value of the culture medium to 6.5-7.5; (3) inoculating the activated strain into the optimized culture medium, and standing and culturing for 8-12 days under the dark condition of 23-27 DEG C; and (4) after the culture is finished, separating and extracting the yellow pigment. By accurately optimizing the carbon source, the nitrogen source, the inorganic salt concentration and culture environment parameters, the yield of the yellow pigment of the chaetomium globosum is remarkably increased, the produced yellow pigment is a natural water-soluble pigment and is high in biological safety, and the culture method is easy and convenient to operate, controllable in cost, good in repeatability, suitable for industrial large-scale production and suitable for industrial large-scale production. And an effective technical support is provided for industrial application of the natural pigment.

The invention discloses a nucleic acid product, a kit and a method for detecting chaetomium globosum and application of the nucleic acid product, the kit and the method in detecting mildewed tobacco leaves. The nucleic acid product comprises a primer pair and a probe for detecting chaetomium globosum, the primer pair comprises an upstream primer with a nucleotide sequence as shown in SEQ ID NO: 1 and a downstream primer with a nucleotide sequence as shown in SEQ ID NO: 2, and the nucleotide sequence of the probe is as shown in SEQ ID NO: 3. The nucleic acid product can specifically detect chaetomium, realizes rapid detection of tobacco mildew based on qPCR, has the advantages of high sensitivity, simple operation, low cost and fast detection speed, can complete the whole detection process within several hours, does not need uncapping in the amplification and detection process, and avoids aerosol pollution caused by uncapping detection in common PCR.

The application discloses a strain of Chaetomium globosum QM, which is named as Chaetomium globosum, and has been preserved in the China General Microbiological Culture Collection Center (CGMCC) (address: No. 1, Xibaheyanli 3, Chaoyang District, Beijing, China Institute of Microbiology) on May 22, 2024, and the strain preservation number is CGMCC NO.41324. The application is a kind of Chaetomium globosum QM separated and purified from wild forest area of Yangshu in Taishan, which is made into fungus powder by using solid fermentation, drying and crushing raw materials, and is applied to crops by seed dressing or flushing, has the effects of promoting growth, disease resistance, stress resistance and the like, and the whole production process does not produce three wastes, has low cost and high benefit, and meets the social needs of environmental protection and grain safety production.

This utility model belongs to the technical field of microbial fermentation and spore extraction system equipment, specifically relating to a sterile material handling device for a *Chaetomium globosum* solid-state fermentation tank. It includes a material handling rod handle, a silicone corrugated tube, and a storage tube connected in sequence, and a material handling rod that passes through the internal cavities of the silicone corrugated tube and the storage tube. One end of the material handling rod is fixedly connected to the handle, and the other end is equipped with a material handling ring. This utility model achieves sealed material handling through the coordinated action of the material handling rod handle, the silicone corrugated tube, the storage tube, and the material handling rod, ensuring no leakage or contamination during material transfer. The silicone corrugated tube, due to its elasticity, fits tightly against the material handling rod, forming a dynamic seal. The elasticity of the silicone corrugated tube allows the material handling rod to move back and forth while maintaining a seal, providing a sterile environment for the rod's movement.

The invention relates to a bisbenzoquinone compound and a preparation method and application thereof.The bisbenzoquinone compound is separated from a fermentation product of chaetomium trilateral, has a brand-new chemical structure, has remarkable inhibitory activity on proliferation of multiple tumor cells, and reaches the nM-level inhibitory activity.

The application provides a chaetomium globosum, a method for separating secondary metabolites of the chaetomium globosum and application, and belongs to the technical field of biological medicines. Arcopilus cupreus DGR-7 has been preserved in Guangdong Microbial Culture Collection Center on October 10, 2025, with a preservation number of GDMCC No: 67068; the application first utilizes rice medium to ferment A. cupreus strains, and 8 compounds are separated and purified from the strains, wherein 6 compounds are anthraquinones. In-vitro antibacterial experiments show that the chaetomium globosum Arcopilus cupreus The secondary metabolites of DGR-7, anthraquinones, have good bioactivity in inhibiting the growth of staphylococcus aureus, methicillin-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, bauman acinetobacter, pseudomonas aeruginosa and escherichia coli.

The invention provides chaetomium hordeum, a method for separating secondary metabolites from chaetomium hordeum and application of the chaetomium hordeum, and belongs to the technical field of biological medicine. The chaetomium cupreum DGR-7 is preserved in the Guangdong Microbial Culture Collection Center on October 10, 2025, and the preservation number of the chaetomium cupreum DGR-7 is GDMCC No: 67068; according to the invention, the rice culture medium is used for fermenting the A.cupreus strain for the first time, and the A.cupreus strain is separated and purified to obtain 8 compounds, including 6 anthraquinone compounds. An in-vitro antibacterial experiment shows that the secondary metabolite anthraquinone compound of the Arcoporus cupreus DGR-7 has good biological activity in the aspect of inhibiting the growth of staphylococcus aureus, methoxyl-resistant staphylococcus aureus, streptococcus salivarius, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa and escherichia coli.

The invention discloses a microbial preparation method of low-molecular-weight water-soluble beta-glucan, and belongs to the technical field of water-soluble beta-glucan preparation, and the microbial preparation method specifically comprises the following steps: carrying out liquid fermentation culture on chaetomium JY25 with the preservation number of CGMCC No.6882 to obtain fermentation liquor; carrying out solid-liquid separation on the fermentation liquor to obtain crude enzyme containing a beta-glucan degrading enzyme system; carrying out enzymolysis reaction on the crude enzyme liquid and an insoluble yeast beta-glucan substrate under the conditions that the pH is 4.0-6.0 and the temperature is 50-65 DEG C to obtain an enzymolysis product; terminating the reaction, and separating and purifying an enzymolysis product to obtain the low-molecular-weight water-soluble beta-glucan with the weight-average molecular weight of 25kDa-30kDa; the invention provides an efficient, green and controllable new method for preparing the low-molecular-weight and water-soluble beta-glucan, and systematically solves multiple limitations of a traditional method in the aspects of efficiency, controllability, environmental protection property and product activity maintenance through combination of a specific functional strain, a synergistic compound enzyme system and an optimized reaction process.

The invention discloses a device and method for producing dextranase through chaetomium gracile fermentation, and relates to the technical field of dextranase production.The device comprises a tank body and further comprises an oscillation stirring part, the oscillation stirring part comprises a rotating shaft which rotates and slides up and down on the tank body, and the rotating shaft is connected with the oscillation stirring part; a stirrer is fixedly mounted at one end, positioned in the tank body, of the rotating shaft; comprising the following steps: S1, taking out a chaetomium graciliforme strain from a glycerol tube at-80 DEG C, inoculating the chaetomium graciliforme strain to a potato dextrose agar slant culture medium in an ultra-clean workbench, and culturing at 28 DEG C for 5 days until a bacterial colony is in an off-white villus shape. The device has the advantages that the growth of thalli and the synthesis of dextranase can be effectively improved through up-and-down reciprocating oscillation, a fermentation sample can be automatically and quantitatively extracted at regular time in the up-and-down reciprocating oscillation process, manual sampling is not needed, improper influence on subsequent fermentation degree judgment caused by misoperation can be avoided, and the working efficiency is improved. And a worker can more accurately analyze and judge the fermentation progress.

The application discloses a chaetomium sublucidum YB-366 for preventing and treating plant parasitic nematodes, a biocontrol agent and application thereof, and aims to solve the technical problem of lacking effective biological agents for effectively preventing and treating plant parasitic nematode diseases. Chaetomium megalocarpum The application screens the chaetomium sublucidum (YB-366) strain with a preservation number of CGMCC No.19646, which has strong lethal and parasitic effects on phillipsonia cyst nematode, southern root knot nematode and scribner short nematode, and the biocontrol agent prepared from the chaetomium sublucidum YB-366 has good prevention and treatment effects on wheat cyst nematode disease, tomato root knot nematode disease and corn root rot nematode disease, and can promote the growth and yield of plants, and is safe, efficient, simple in method and low in prevention and treatment cost.

The invention discloses chaetomium cochliodes GRQ-2 and application of the chaetomium cochliodes GRQ-2, the chaetomium cochliodes GRQ-2 is separated from tobacco plants and has an excellent prevention effect on tobacco floating seedling root rot, and the morbidity is only 22.22%; the confrontation culture shows that the strain GRQ-2 has an obvious inhibition effect on the growth of the trichoderma trefoil, and the bacteriostasis rate can reach 82.09%; in addition, the antagonistic activity of the strain GRQ-2 on pathogenic fungi of 12 test plants (including tobacco, pepper and the like) is detected through a plate confrontation method, and the bacteriostasis rate ranges from 37.83% to 74.12%. In conclusion, the chaetomium cochliodes GRQ-2 disclosed by the invention has broad-spectrum disease resistance and strong environmental adaptability, and can be used for effectively preventing and treating the tobacco floating seedling root rot.