Chaetomium globosum, fungicide, seed soaking solution and application thereof

A technology of Chaetomium and seed soaking solution, applied in the field of agricultural biology, can solve problems such as the inability of conventional crops to produce broad-spectrum synergy, and achieve the goal of improving drought resistance and salinity resistance, increasing germination speed, and promoting crop growth. Effect

Active Publication Date: 2021-08-10
MOON (GUANGZHOU) BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing patents have the following problems: most bacterial strains can only be effective against

Method used

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  • Chaetomium globosum, fungicide, seed soaking solution and application thereof
  • Chaetomium globosum, fungicide, seed soaking solution and application thereof
  • Chaetomium globosum, fungicide, seed soaking solution and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0093] This example provides screening, isolation and identification methods for Chaetomium globosum MN110495 strain.

[0094] (1) Screening and isolation of strains:

[0095] The samples came from rhizome soil collected from cotton fields in Henan Province. Weigh 10g of dry soil, put the soil into a sterilized mortar and pound it with a pestle, place it on the gauze and rinse it with tap water, move the clay attached to the gauze to a centrifuge tube, add 50ml of sterile water, vortex, and centrifuge at 10000rpm After 6 minutes, discard the supernatant, wash with sterile water, and centrifuge again to allow the particles to settle to the bottom of the conical centrifuge tube, and repeat centrifugation for 3 times. Get 1 g of the above-mentioned cleaned soil and dilute the precipitate with a suspension made of 20 mL of sterile sodium carboxymethyl cellulose and apply it on the RBM medium, and cultivate it upside down in the dark at room temperature for 7 days. The mycelia we...

Embodiment 2

[0104] In this example, the fermentation broth of Chaetomium coccidioides MN110495 strain was prepared.

[0105] Use a sterile puncher to punch 18 holes. The bacteria obtained from the separation and screening in Example 1 were placed in a 50mL sterile centrifuge tube containing 30mL of PDB culture solution, placed in a constant temperature shaking incubator, and cultivated at 28°C and 200rmp. 4d, count the number of spores with a hemocytometer, and adjust the number of spores in the fermentation broth to 10 6 CFU / mL.

Embodiment 3

[0107] In this example, the effect of Chaetomium coccidioides MN110495 strain on the drought resistance of wheat seeds during germination was studied. Technical route reference figure 2 shown.

[0108] Take out wheat seeds (Jimai 23) from the cold storage, select healthy seeds with consistent particle size, disinfect with 75% ethanol for 1 min, wash with sterile water for 3 times, then disinfect with 5% sodium hypochlorite for 5 min, and finally wash with sterile water for 3 all over. Then use the treatment solution to carry out seed soaking treatment at room temperature. According to the different treatment solutions, set up the experimental group and the blank control group.

[0109] The experimental groups were MN110495 treatment group, negative control group and positive control group, wherein the MN110495 treatment group used the strain fermentation liquid in Example 2 to soak seeds overnight; the negative control group used PDB culture solution to soak seeds; the pos...

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Abstract

The invention discloses chaetomium globosum, a fungicide, a seed soaking solution and an application thereof, and relates to the technical field of agricultural biology. The strain is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC No: 61363, and the taxonomic name of the strain is Chaetomium globosum. Experiments prove that the strain has broad spectrum for improving the stress resistance of crops, and can obviously improve the drought resistance and saline-alkali resistance of the crops. Under the stress of a drought environment, the chaetomium globosum MN110495 can generate substances such as exopolysaccharides and antioxidants to resist the drought environment, so that the drought resistance of various plants such as fruits, vegetables and food crops is improved. The chaetomium globosum also has the effects of promoting seed germination and crop growth.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a Chaetomium coccidioides, bacterial agent, seed soaking solution and application. Background technique [0002] The shortage of water resources is a global problem currently restricting agricultural production. With the rapid development of global industrialization, frequent natural disasters, and reduced precipitation, about 43% of the arable land is in dry or semi-dry areas. Dry and early adversity seriously affects the growth of plants. development, resulting in crop yield reduction. At present, the agricultural field mainly uses seaweed fertilizers, amino acid water-soluble fertilizers, and plant growth regulators to assist crops in drought resistance, and humic acid is one of their representatives. Humic acid fertilizer is a kind of chemical fertilizer made from humic acid-rich peat and weathered coal as the main raw materials, after chemical treatment such as amm...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01C1/00C05G3/00A01N63/30A01P21/00C12R1/645
CPCC12N1/14A01C1/00C05G3/00A01N63/30
Inventor 张颖施陈娟陈泽怡彭锦胜梁均钿
Owner MOON (GUANGZHOU) BIOTECH CO LTD
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