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Application of chaetomium globosum in preventing and treating wheat take-all

A technology for wheat take-all and Chaetomium globosa, applied in application, seed coating/seed dressing, fungicides, etc., to achieve the effects of reducing disease grade, reducing disease index, and increasing plant height

Active Publication Date: 2020-11-03
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of isolated Chaetomium globosa to control wheat take-all

Method used

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  • Application of chaetomium globosum in preventing and treating wheat take-all
  • Application of chaetomium globosum in preventing and treating wheat take-all

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation of Chaetomium globosa Strain 12XP1-2-3

[0019] At the wheat grain filling stage, the unaffected plants in the wheat take-all field were collected in Xiping County, Henan Province and put into paper bags. The samples were stored in a refrigerator at 4°C. Rinse the root of the sample, and then take 2-3cm of wheat root tissue, disinfect the surface with 75% absolute ethanol for 10-30s, disinfect with 1% NaCIO for 1.5min, rinse with sterile water for 3 times, and blot dry with sterile filter paper After hydration, cut into small sections of about 0.5 cm, and place them on a potato dextrose agar medium (PDA, raw material composition: 300 g of peeled potatoes, 20 g of glucose, 20 g of agar and 1000 ml of distilled water) with sterile tweezers. After 5-7 days of cultivation under the same conditions, pick off the edge mycelia and grow on a new PDA plate. After 5-7 days under the same conditions, pick off the mycelium pieces with uniform colonies and place them on a...

Embodiment 2

[0021] Extraction of DNA from Chaetomium globosa Strain 12XP1-2-3

[0022] Pick the chaetomium strain 12XP1-2-3 onto the PDA medium, and after culturing at 20°C for 5-7 days, pick 5 edge mycelium pieces and place them evenly on the PDA plate covered with sterilized cellophane. After 3-5 days, scrape the mycelium with a sterilized small shovel, freeze it with liquid nitrogen, and store it in a refrigerator at -20°C. When extracting DNA, take out 20-25 mg mycelium and put it into a pre-cooled 1.5ml EP tube; add a little liquid nitrogen and grind the mycelium to powder in the EP tube with a pre-cooled iron nail; add 500 μl Extraction Buffer (50mM Tris -Cl (pH 8.0), 150mM NaCl, 100mM EDTA (8.0)), shake the suspended pellet on a vortex shaker and mix well; , mixed upside down, placed in a water bath at 37°C for 1-3 hours; then added 75 μl of 5M NaCl, mixed upside down; added 65 μl of CTAB / NaCl (10% CTAB, 0.7M NaCl) solution, bathed in water at 65°C for 30 minutes ; Add an equal v...

Embodiment 3

[0024] Molecular identification of Chaetomium globosa strain 12XP1-2-3

[0025] In this test, primers ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS4 (5'-TCC TCCGCT TAT TGA TAT GC-3') were used to amplify the strains containing ITS1, 5.8S rDNA and ITS2 of DNA fragments. The system refers to Daval et al. (2010). Amplification reaction conditions: The PCR reaction program is: 95°C for 3min; 95°C for 45s, 50°C for 30s, 72°C for 1min, 35 cycles; 72°C for 10min. After the PCR products were sequenced, BLAST sequence alignment analysis was carried out in NCBI. The result of the sequence alignment shows that the biocontrol bacterium 12XP1-2-3 is a globular hair shell.

[0026] Table 1. PCR reaction system

[0027] Element concentration Dosage (μl) 10×PCR buffer 10mM 5.0 dNTP mix 10mM 1.0 TaqDNase 5u 0.5 Primer 1 20μM 1.0 Primer 2 20μM 1.0 DNA template 10-50ng / μl 1.0 double distilled water pure water 40.5 Tot...

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Abstract

The invention provides an application of chaetomium globosum in prevention and treatment of wheat take-all, the preservation name of the chaetomium globosum is 12XP1-2-3, the preservation number is CGMCC No.17183, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is January 23, 2019. The inhibition rate of the chaetomium globosum 12XP123 onwheat take-all pathogens GaNS90 on a flat plate is 45.4%, and field test results show that when the chaetomium globosum 12XP1-2-3 is used for wheat seed coating treatment, the white spike rate and the disease index of wheat can be obviously reduced.

Description

technical field [0001] The invention relates to the application of chaetomium globosa in preventing and treating wheat take-all disease. Background technique [0002] Take-all is an important root disease of wheat, which can cause clusters or large areas of wheat plants to die, and reduce the number of effective panicles, grains per panicle and thousand-grain weight. Wheat can be infected at both seedling and adult stages. Typical symptoms are blackening of wheat roots and stem bases, also known as "black foot disease", and flaky white spikes appear in the field during the filling period. [0003] For a long time, control measures such as chemical seed dressing have been used to prevent and control. However, long-term and excessive use of chemical agents will not only damage the soil micro-ecological environment, aggravate environmental pollution, but also easily delay the emergence of wheat seedlings and reduce the emergence rate. Therefore, the focus of disease control h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/30A01P3/00C12N1/22C12N1/14A01C1/06A01G22/20C12R1/645
CPCA01N63/30C12N1/22C12N1/14A01C1/06A01G22/20
Inventor 徐飞宋玉立王俊美张姣姣韩自行李亚红李丽娟刘露露
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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