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Application of a kind of Chaetomium globosa in the prevention and treatment of wheat stem rot

A technique for Chaetomium globosa, stem base rot in applications, microbial-based methods, fungicides, etc.

Active Publication Date: 2022-03-04
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of isolated Chaetomium globosa to control wheat stem rot

Method used

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  • Application of a kind of Chaetomium globosa in the prevention and treatment of wheat stem rot
  • Application of a kind of Chaetomium globosa in the prevention and treatment of wheat stem rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Isolation of Chaetomium globosa Strain 12XP1-2-3

[0019] At the wheat grain filling stage, the unaffected plants in the wheat take-all field were collected in Xiping County, Henan Province and put into paper bags. The samples were stored in a refrigerator at 4°C. Rinse the root of the sample, and then take 2-3cm of wheat root tissue, disinfect the surface with 75% absolute ethanol for 10-30s, disinfect with 1% NaCIO for 1.5min, rinse with sterile water for 3 times, and blot dry with sterile filter paper After hydration, cut into small sections of about 0.5 cm, and place them on a potato dextrose agar medium (PDA, raw material composition: 300 g of peeled potatoes, 20 g of glucose, 20 g of agar and 1000 ml of distilled water) with sterile tweezers. After 5-7 days of cultivation under the same conditions, pick off the edge mycelia and grow on a new PDA plate. After 5-7 days under the same conditions, pick off the mycelium pieces with uniform colonies and place them on a...

Embodiment 2

[0021] Extraction of DNA from Chaetomium globosa Strain 12XP1-2-3

[0022] Pick the chaetomium strain 12XP1-2-3 onto the PDA medium, and after culturing at 20°C for 5-7 days, pick 5 edge mycelium pieces and place them evenly on the PDA plate covered with sterilized cellophane. After 3-5 days, scrape the mycelium with a sterilized small shovel, freeze it with liquid nitrogen, and store it in a refrigerator at -20°C. When extracting DNA, take out 20-25mg mycelium and put it into a pre-cooled 1.5ml EP tube; add a little liquid nitrogen and grind the mycelium to powder in the EP tube with a pre-cooled iron nail; add 500μl Extraction Buffer (50mM Tris- Cl (pH 8.0), 150mM NaCl, 100mM EDTA (8.0)), oscillated on a vortex shaker to suspend the precipitate and mix well; Mix it upside down, and place it in a water bath at 37°C for 1-3 hours; then add 75 μl of 5M NaCl, mix it upside down; add 65 μl of CTAB / NaCl (10% CTAB, 0.7M NaCl) solution, and bathe in water at 65°C for 30 minutes; A...

Embodiment 3

[0024] Molecular identification of Chaetomium globosa strain 12XP1-2-3

[0025] In this test, primers ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS4 (5'-TCC TCCGCT TAT TGA TAT GC-3') were used to amplify the strains containing ITS1, 5.8S rDNA and ITS2 of DNA fragments. The system refers to Daval et al. (2010). Amplification reaction conditions: The PCR reaction program is: 95°C for 3min; 95°C for 45s, 50°C for 30s, 72°C for 1min, 35 cycles; 72°C for 10min. After the PCR products were sequenced, BLAST sequence alignment analysis was carried out in NCBI. The result of the sequence alignment shows that the biocontrol bacterium 12XP1-2-3 is a globular hair shell.

[0026] Table 1. PCR reaction system

[0027] Element concentration Dosage (μl) 10×PCR buffer 10mM 5.0 dNTP mix 10mM 1.0 TaqDNase 5u 0.5 Primer 1 20μM 1.0 Primer 2 20μM 1.0 DNA template 10-50ng / μl 1.0 double distilled water pure water 40.5 Tot...

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Abstract

The invention provides an application of Chaetomium globosa in preventing and treating wheat stem base rot. The preservation name of the Chaetomium globosa is 12XP1-2-3, the preservation number is CGMCC No.17183, and the preservation unit is China Microbial Bacteria The General Microbiology Center of the Species Depository Management Committee, the deposit date is January 23, 2019. The field test results showed that using Chaetomium globulus 12XP1‑2‑3 to coat wheat seeds could reduce the diseased stem rate, disease grade and disease index by 19.0%‑41.3%, 43.7% and 4.7%‑45.4%, respectively.

Description

technical field [0001] The invention relates to the application of chaetomium globosa in preventing and treating wheat stem rot. Background technique [0002] Wheat stalk rot is an important root disease of wheat, which can reduce the weight of spikes and 1,000-grain. It can infect wheat at the seedling stage, tillering stage, jointing stage, and adult plant stage. The typical symptom is that the base of the wheat stem turns brown and becomes the color of soy sauce. , Scattered white spikes appeared in the field during the filling period. [0003] For wheat stalk rot, there are currently no effective control agents. The use of existing chemical agents will not only damage the soil micro-ecological environment and aggravate environmental pollution, but some seed dressing agents will also delay the emergence of wheat seedlings and affect the emergence of seedlings. Therefore, the focus of disease control has gradually shifted to biological control and agricultural control mea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P3/00C12R1/645
CPCC12N1/14A01N63/30C12R2001/645C12N1/145A01N65/00A01P3/00A01N25/00A01N25/04C12N15/00
Inventor 徐飞宋玉立王俊美张姣姣韩自行李亚红石瑞杰李丽娟刘露露
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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