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Solid-state fermentation method of chaetomium globosum, prepared microbial inoculum and application of microbial inoculum

A technology of Chaetomium globosa and solid-state fermentation, applied in the direction of microorganism-based methods, applications, fungicides, etc., can solve the problems affecting the quality and safety of agricultural products, the prevention and control of soil-borne diseases is severe, and the indicators do not cure the root cause, so as to reduce the occurrence of crop diseases , significant antagonistic effect, obvious effect

Active Publication Date: 2020-11-17
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The prevention and control of soil-borne diseases is mainly to inhibit the activity of pathogens in the rhizosphere system to protect the root system. Although the application of chemical pesticides has a control effect, there are pesticide residues, which affect the quality and safety of agricultural products.
In addition, the use of cultivation technology innovation and the cultivation of resistant crop varieties also have a certain control effect, but the indicators do not cure the root cause, and the prevention and control of soil-borne diseases is still severe

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The shallow plate solid-state culture of Chaetomium globosa HY1

[0025] A ring of Chaetomium globosa strain HY1 preserved on the slant of the test tube was picked with an inoculation loop, inoculated in the activation medium, and cultured at 28°C and 200 rpm for 26 h until the cells became turbid to obtain the activated seed solution. The activation medium is: 1.5% soluble starch, 0.6% yeast powder, 0.4% corn steep liquor, 0.1% manganese sulfate, 0.2% magnesium chloride, pH 7.5.

[0026] According to the inoculum amount of 8% (v / v), the activated seed liquid was inserted into the sterilized solid fermentation medium, and the activated bacteria were mixed with the solid medium evenly, and then placed in a sterile shallow plate incubator, at 28°C Under static culture, sterile air was introduced once every 8 hours, and the ventilation rate was 1L of medium through 3L of sterile air meter; after 3 days of cultivation, the surface of the culture medium was cov...

Embodiment 2

[0028] Embodiment 2: the fermenter solid-state culture of Chaetomium globosa HY1

[0029] Pick a loop of Chaetomium globosa strain HY1 preserved on the slant of the test tube with an inoculation loop, inoculate it in the activation medium, and cultivate it at 28°C and 200 rpm until the bacteria are turbid to obtain the activated seed solution. The activation medium is: 1% soluble starch, 0.4% yeast powder, 0.2% corn steep liquor, 0.2% manganese sulfate, 0.1% magnesium chloride, pH 7.5.

[0030] The solid-state fermentation medium was filled into a 50 L solid-state fermenter with a liquid content of 65%, sterilized in situ at 118°C for 20 minutes, and cooled to room temperature for later use. The solid-state fermentation medium is: 20% dried brewer's grains, 15% bran, 1% glycerin, 1.2% tryptone, 0.6% Na 2 HPO 4 , 0.3% K 2 HPO 4 , 1% chitin powder, the ratio of material to water is 1:1.5, and the pH is natural.

[0031] Insert the activated seed culture solution according t...

Embodiment 3

[0033] Embodiment 3: the solid-state culture of the ventilated fermentation tank of Chaetomium globosa HY1

[0034] Pick a loop of Chaetomium globosa strain HY1 preserved on the slant of the test tube with an inoculation loop, inoculate it in the activation medium, and cultivate it at 28°C and 200 rpm until the bacteria are turbid to obtain the activated seed solution. The mass percentage of the activation medium is: 1.2% soluble starch, 0.5% yeast powder, 0.3% corn steep liquor, 0.15% manganese sulfate, 0.15% magnesium chloride, pH 7.5.

[0035] The solid-state fermentation medium is loaded with 75% liquid volume into 1m 3 In a solid fermentation tank, sterilize in situ at 115°C for 25 minutes, and cool to room temperature for later use. The solid-state fermentation medium is: 18% dried brewer's grains, 12% bran, 0.8% glycerin, 1% tryptone, 0.5% Na 2 HPO 4 , 0.5%K 2 HPO 4 , 1.2% chitin powder, the ratio of material to water is 1:0.82, and the pH is natural.

[0036]The ...

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PUM

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Abstract

The invention discloses a solid state fermentation method of chaetomium globosum, a prepared microbial inoculum and an application of the microbial inoculum. The chaetomium globosum HY1 is adopted toperform strain activation, then solid fermentation is performed, after the solid fermentation is ended, a solid culture is scattered and uniformly mixed, and the mixture is placed in a shady, cool andventilated place to be aired and crushed to obtain the agricultural composite biocontrol microbial inoculum, and the microbial inoculum is used for preventing and treating agricultural root-knot nematodes and soil-derived phytopathogens. The process is simple to operate, parameters are easy to control, the composite biocontrol microbial inoculum produced by solid-state fermentation has high capacity of killing root-knot nematodes and soil-derived phytopathogens, and has wide application prospect and remarkable economic and social benefits.

Description

technical field [0001] The invention relates to a solid-state fermentation method of Chaetomium globosa and the application of the bacterial agent prepared by the same to the prevention and control of agricultural root-knot nematodes and soil-derived plant pathogenic bacteria, belonging to the technical field of microbial pesticides. Background technique [0002] In the process of large-scale and intensive cultivation of modern agriculture, due to reasons such as continuous crops and improper fertilization, soil-borne pathogens often occur. Pathogens invade crops from roots or stems when conditions are suitable, and infect pathogens including fungi. , bacteria, actinomycetes, nematodes, etc., causing huge losses to agricultural production, such as Pythium causing seedling rot and damping-off, Rhizoctonia causing seedling blight, and crops such as Fusarium oxysporum and Verticillium wilt wilting, dying, etc. [0003] In addition to the invasion of soil-borne pathogenic micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/30A01P1/00A01P3/00A01P5/00C12R1/645
CPCC12N1/14A01N63/30C12R2001/645C12N1/145
Inventor 程仕伟张萍冯志彬葛宜和毛志远姜南缪静
Owner LUDONG UNIVERSITY
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