Solid-state fermentation method of chaetomium globosum, prepared microbial inoculum and application of microbial inoculum
A technology of Chaetomium globosa and solid-state fermentation, applied in the direction of microorganism-based methods, applications, fungicides, etc., can solve the problems affecting the quality and safety of agricultural products, the prevention and control of soil-borne diseases is severe, and the indicators do not cure the root cause, so as to reduce the occurrence of crop diseases , significant antagonistic effect, obvious effect
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Embodiment 1
[0024] Embodiment 1: The shallow plate solid-state culture of Chaetomium globosa HY1
[0025] A ring of Chaetomium globosa strain HY1 preserved on the slant of the test tube was picked with an inoculation loop, inoculated in the activation medium, and cultured at 28°C and 200 rpm for 26 h until the cells became turbid to obtain the activated seed solution. The activation medium is: 1.5% soluble starch, 0.6% yeast powder, 0.4% corn steep liquor, 0.1% manganese sulfate, 0.2% magnesium chloride, pH 7.5.
[0026] According to the inoculum amount of 8% (v / v), the activated seed liquid was inserted into the sterilized solid fermentation medium, and the activated bacteria were mixed with the solid medium evenly, and then placed in a sterile shallow plate incubator, at 28°C Under static culture, sterile air was introduced once every 8 hours, and the ventilation rate was 1L of medium through 3L of sterile air meter; after 3 days of cultivation, the surface of the culture medium was cov...
Embodiment 2
[0028] Embodiment 2: the fermenter solid-state culture of Chaetomium globosa HY1
[0029] Pick a loop of Chaetomium globosa strain HY1 preserved on the slant of the test tube with an inoculation loop, inoculate it in the activation medium, and cultivate it at 28°C and 200 rpm until the bacteria are turbid to obtain the activated seed solution. The activation medium is: 1% soluble starch, 0.4% yeast powder, 0.2% corn steep liquor, 0.2% manganese sulfate, 0.1% magnesium chloride, pH 7.5.
[0030] The solid-state fermentation medium was filled into a 50 L solid-state fermenter with a liquid content of 65%, sterilized in situ at 118°C for 20 minutes, and cooled to room temperature for later use. The solid-state fermentation medium is: 20% dried brewer's grains, 15% bran, 1% glycerin, 1.2% tryptone, 0.6% Na 2 HPO 4 , 0.3% K 2 HPO 4 , 1% chitin powder, the ratio of material to water is 1:1.5, and the pH is natural.
[0031] Insert the activated seed culture solution according t...
Embodiment 3
[0033] Embodiment 3: the solid-state culture of the ventilated fermentation tank of Chaetomium globosa HY1
[0034] Pick a loop of Chaetomium globosa strain HY1 preserved on the slant of the test tube with an inoculation loop, inoculate it in the activation medium, and cultivate it at 28°C and 200 rpm until the bacteria are turbid to obtain the activated seed solution. The mass percentage of the activation medium is: 1.2% soluble starch, 0.5% yeast powder, 0.3% corn steep liquor, 0.15% manganese sulfate, 0.15% magnesium chloride, pH 7.5.
[0035] The solid-state fermentation medium is loaded with 75% liquid volume into 1m 3 In a solid fermentation tank, sterilize in situ at 115°C for 25 minutes, and cool to room temperature for later use. The solid-state fermentation medium is: 18% dried brewer's grains, 12% bran, 0.8% glycerin, 1% tryptone, 0.5% Na 2 HPO 4 , 0.5%K 2 HPO 4 , 1.2% chitin powder, the ratio of material to water is 1:0.82, and the pH is natural.
[0036]The ...
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