Application of chaetomium elatum in denitration of nitrocellulose

A technology of nitrocellulose and Chaetomium, which is applied in environmental engineering and biological fields, can solve the problems of complex and cumbersome, inconvenient nitrogen reduction mechanism, unclear mechanism of nitrocellulose, etc., and achieves simple control, good denitrification and denitrification. Effect

Inactive Publication Date: 2021-06-01
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method is relatively complicated and cumbersome, and the mechanism of microbial nitrogen reduction on nitrocellulose is not clear, so it is difficult to meet the requirements of industrial treatment.
Moreover, most of the microorganisms in the sludge perform anaerobic respiration and facultative anaerobic respiration, which is very inconvenient for studying the specific mechanism of nitrogen reduction.

Method used

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  • Application of chaetomium elatum in denitration of nitrocellulose
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  • Application of chaetomium elatum in denitration of nitrocellulose

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Experimental program
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Effect test

Embodiment 1

[0034] Embodiment 1: the preparation of spore suspension and the cultivation of bacterial strain

[0035] This study will come from China General Microorganism Culture Collection Center, the preservation number is CGMCC NO: 3.5617 a strain of Chaetomium tall ( Chaetomium elatum ), insert the culture medium from the inclined surface of the test tube, and use the spore suspension to inoculate.

[0036] The preparation of spore suspension: will come from China General Microorganism Culture Collection Center, preservation number is a strain of chaetomium tall (CGMCC NO:3.5617) Chaetomium elatum ), use an inoculation loop to take a ring of spores from the inclined surface of the test tube, and add 1 mL of sterile water to prepare a spore suspension.

[0037] Liquid culture of strains: 200 µL of spore suspension was added to 100 mL of liquid potato medium for cultivation.

[0038] The composition of the potato medium is as follows: glucose 20.0, unit g / L; potato extract 1000 mL (2...

Embodiment 2

[0043] Embodiment 2: Chaetomium tall under different days ( Chaetomium elatum ) compared to the nitrogen reduction results of nitrocellulose

[0044] Will be purchased from China General Microorganism Culture Collection Center, a strain of Chaetomium tall ( Chaetomium elatum ), use an inoculation loop to take a ring of spores from the slant of the test tube, add 1 mL of sterile water to make a spore suspension, and then add 200 µL of the spore suspension to 100 mL of liquid potato medium for cultivation. In a shaker at 28°C, culture at 200rpm for 2 days until visible cells are formed, and then add 4g / L nitrocellulose as a substrate to carry out substrate conversion reaction with the tall chaetomium strain.

[0045] After the conversion reaction was carried out for 7 days, the cultured substrate nitrocellulose was re-extracted using a suction filtration device to reduce the loss rate of nitrocellulose. After the suction filtration, take the precipitate on the filter paper; wash...

Embodiment 3

[0047] Embodiment 3: Chaetomium tall under different days ( Chaetomium elatum ) treated nitrocellulose FTIR results comparison.

[0048] The Fourier transform infrared spectra of the nitrocellulose treated with sulfate-reducing bacteria for different days and the original nitrocellulose were compared. 1635cm -1 -ONO 2 Asymmetric stretching vibration, 1274 cm -1 -ONO 2 Symmetric stretching vibration, 1061 cm -1 C-O-C stretching vibration at 996 cm -1 C-O-C stretching vibration at 824 cm -1 is C-O-C stretching vibration. It was found that the main groups of nitrocellulose did not disappear, and no new groups were produced, and the absorption peak of nitro was in Chaetomium tallum ( Chaetomium elatum ) significantly decreased after treatment, and the longer the time, the more obvious the decrease. For details, see figure 2 .

[0049] The denitrification and denitrification of nitrocellulose by the tall Chaetomium strain described in the present invention realizes th...

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Abstract

The invention discloses an application of chaetomium elatum in denitration of nitrocellulose. The application is characterized in that a chaetomium elatum strain converts nitrocellulose, denitration is completed in the conversion process, and nitrate and nitrite can be converted into own nutrient substances in the conversion process, so that better growth of the strain is achieved, and pollution is reduced; a culture medium is simple in components, readily available in raw materials and suitable for industrial production; and nitrocellulose is subjected to denitration treatment by using a single microbial strain, so that the denitrification degree of the nitrocellulose can be controlled more simply and conveniently, and a good foundation is laid for later research on the nitrogen reduction mechanism of the nitrocellulose. According to the invention, alkali hydrolysis pretreatment is not needed before denitration conversion, and a cellulose main chain cannot be damaged; no anaerobic environment is needed in the denitration process of the chaetomium elatum on the nitrocellulose, and the conversion environment is relatively simple; after the strain is co-cultured with nitrocellulose for 14 days, the nitrogen content of nitrocellulose with the original nitrogen content of 13.03% can be reduced to 10.43%.

Description

technical field [0001] The invention belongs to the fields of environmental engineering and biology, and relates to the application of a chaetomium tall in denitrification of nitrocellulose. Background technique [0002] Nitrocellulose is a high molecular polymer material formed by esterification of cellulose and concentrated nitric acid. It has a wide range of applications, such as coatings, celluloid, and propellants. Nitrocellulose per monomer C 6 h 7 o 2 (ONO 2 ) 3 The contained nitrogen content is 14.1%. Nitrocellulose with too high nitrogen content has unstable performance and is extremely explosive, so it is necessary to seek a method to reduce the nitrogen content of nitrocellulose. [0003] Chemically, the nitrogen reduction treatment method for nitrocellulose is alkaline hydrolysis, and the nitrate and nitrite produced by alkaline hydrolysis will cause great pollution to soil and water quality. In addition, it is difficult to control the degree of reaction by...

Claims

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Application Information

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IPC IPC(8): A62D3/02A62D101/06A62D101/26
CPCA62D3/02A62D2101/06A62D2101/26
Inventor 陈可泉陈乔煜张阿磊丁亚军肖忠良欧阳平凯
Owner NANJING UNIV OF TECH
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