Chaetomium globosum and application thereof in promoting growth and increasing yield of wheat

A technology of Chaetomium globosa and wheat grains, applied in the direction of application, microbial-based methods, and microbial-using methods, can solve problems that have not been reported, and achieve the effect of increasing yield and increasing chlorophyll content

Inactive Publication Date: 2020-12-15
INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the use of isola...

Method used

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  • Chaetomium globosum and application thereof in promoting growth and increasing yield of wheat
  • Chaetomium globosum and application thereof in promoting growth and increasing yield of wheat
  • Chaetomium globosum and application thereof in promoting growth and increasing yield of wheat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Isolation of Chaetomium globosa Strain 12XP1-2-3

[0023] At the wheat grain filling stage, the uninfected plants in the wheat fields were collected in Xiping County, Henan Province and put into paper bags. The samples were stored in a refrigerator at 4°C. Rinse the root of the sample, and then take 2-3cm of wheat root tissue, disinfect the surface with 75% absolute ethanol for 10-30s, disinfect with 1% NaCIO for 1.5min, rinse with sterile water for 3 times, and blot dry with sterile filter paper After hydration, cut into small sections of about 0.5 cm, and place them on a potato dextrose agar medium (PDA, raw material composition: 300 g of peeled potatoes, 20 g of glucose, 20 g of agar and 1000 ml of distilled water) with sterile tweezers. After 5-7 days of cultivation under the same conditions, pick off the edge mycelia and grow on a new PDA plate. After 5-7 days under the same conditions, pick off the mycelium pieces with uniform colonies and place them on a PDA sla...

Embodiment 2

[0025] Extraction of DNA from Chaetomium globosa Strain 12XP1-2-3

[0026] Pick the chaetomium strain 12XP1-2-3 onto the PDA medium, and after culturing at 20°C for 5-7 days, pick 5 edge mycelium pieces and place them evenly on the PDA plate covered with sterilized cellophane. After 3-5 days, scrape the mycelium with a sterilized small shovel, freeze it with liquid nitrogen, and store it in a refrigerator at -20°C. When extracting DNA, take out 20-25 mg mycelium and put it into a pre-cooled 1.5ml EP tube; add a little liquid nitrogen and grind the mycelium to powder in the EP tube with a pre-cooled iron nail; add 500 μl Extraction Buffer (50mM Tris -Cl (pH 8.0), 150mM NaCl, 100mM EDTA (8.0)), shake the suspended pellet on a vortex shaker and mix well; , mixed upside down, placed in a water bath at 37°C for 1-3 hours; then added 75 μl of 5M NaCl, mixed upside down; added 65 μl of CTAB / NaCl (10% CTAB, 0.7M NaCl) solution, bathed in water at 65°C for 30 minutes ;Add an equal vo...

Embodiment 3

[0028] Molecular identification of Chaetomium globosa strain 12XP1-2-3

[0029] In this test, primers ITS1 (5'-TCC GTA GGT GAA CCT GCG G-3') and ITS4 (5'-TCC TCCGCT TAT TGA TAT GC-3') were used to amplify the strains containing ITS1, 5.8S rDNA and ITS2 of DNA fragments. The system refers to Daval et al. (2010). Amplification reaction conditions: The PCR reaction program is: 95°C for 3min; 95°C for 45s, 50°C for 30s, 72°C for 1min, 35 cycles; 72°C for 10min. After the PCR products were sequenced, BLAST sequence alignment analysis was carried out in NCBI. The result of the sequence alignment shows that the biocontrol bacterium 12XP1-2-3 is a globular hair shell.

[0030] Table 1. PCR reaction system

[0031]

[0032]

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Abstract

The invention provides chaetomium globosum (Chaetomium globosum), which has a preservation name of 12XP1-2-3 and a preservation number of CGMCC No.17183, wherein the preservation unit is the China general microbiological culture collection centre; and the preservation date is January 23th, 2019. The invention further provides application of chaetomium globosum in promoting growth and increasing yield of wheat. Field test results show that: when the chaetomium globosum 12XP1-2-3 provided by the invention is used for wheat seed coating treatment, the chlorophyll content of leaves can be increased by 1.9%-14.7% in the booting stage; and the wheat yield can be increased by 2.3%-10.9%.

Description

technical field [0001] The invention relates to a chaetomium globosa and its application in promoting growth and increasing production of wheat. Background technique [0002] Agricultural production has high requirements on wheat yield, and the use of microbial inoculum seed dressing to promote wheat growth and increase yield is a hot research direction at present. Chaetomium sp. fungi are widely distributed in soil and plants. This type of microorganism can colonize the roots of wheat and promote the nutrient absorption of plants. At present, there is no report on using the isolated Chaetomium globosa to promote the growth and yield of wheat. Contents of the invention [0003] The invention provides a kind of Chaetomium globosa 12XP1-2-3, which can be used to promote the growth and increase of wheat. The field drug efficacy test results show that after coating wheat seeds with Chaetomium globosa 12XP1-2-3, the booting stage It can increase the chlorophyll content of le...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/22A01N63/30A01P21/00A01C1/06C12R1/645
CPCC12N1/14C12N1/22A01N63/30A01C1/06C12R2001/645C12N1/145A01P21/00
Inventor 宋玉立徐飞王俊美韩自行李亚红冯超红
Owner INST OF PLANT PROTECTION HENAN ACAD OF AGRI SCI
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