Method of industrial quick production and preparation of glucosyl stevioside mixture

Abstract

The invention provides a method of industrial quick production and preparation of a glucosyl stevioside mixture. The method comprises the following steps of: step I, performing enzymatic reaction: dissolving oxidized starch and stevioside, adding CGT (cyclodextrin glycosyltransferase) for the enzymatic reaction at 40-55 DEG C for 7-12h, and performing enzyme deactivation to finish the reaction, step II, performing decoloration ad deodorization, and step III, performing filtration, concentration and drying to form a product. The method takes the oxidized starch as a glycosyl donor; technical steps are reduced; a transglycosylation effect is improved; and the method is simple in technology and easy to operate, reduces requirements of industrialization on equipment and energy consumption at the same time, shortens working hours, lowers the cost and increases unit production capacity.

Description

technical field [0001] The invention belongs to the technical field of stevioside production technology, and more specifically relates to an industrial rapid production method for preparing a glucosyl steviol glycoside mixture. Background technique [0002] With the promotion of stevia, people found that it has a bitter aftertaste. Although stevia has many advantages, under the influence of people's taste habits formed over the years, its economic benefits have been seriously affected in some areas. shock. In recent years, the research on the improvement of stevia sugar taste quality has become another hot issue after the safety research of stevia sugar; The sugar industry reaches new heights. [0003] In the prior art, the overall yield of the product obtained by enzymatically modifying stevioside with glucosyltransferase, fructofuranosidase, and galactosidase is low, and the unit production capacity is low; the proportion of more than 2 grape-based products grafted at th...

AI Technical Summary

Problems solved by technology

[0005] However, this technical solution has certain limitations and is not suitable for industrial production: three different enzyme treatments are required, the steps are cumbersome, and three different enzymes are used, the enzyme dosage and cost are too high; For high-temperature treatment, it is necessary to boil at high temperature for 5 minutes at an acidity of pH 2.8 for the first time, which will cause greater damage to iron-containing equipment, or require higher-quality and more expensive acid-resistant equipment, and also affect the temperature control system. There are also higher requirements, high temperature inactivation of enzymes needs to consume more energy and increase carbon emissions; 3 times of enzyme treatment takes a long time, and the total time of 3 times of enzyme treatment exceeds 37 hours, which is inefficient

Due to the 3 times of enzyme treatment, more impurities were introduced, and the grafting effect was not ideal. There were still more starch enzymatic products that still needed to go through steps such as macroporous resin removal, ion exchange resin desalination, etc., which further extended the working hours and increased cost

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