A method for constructing three-dimensional glioma tissue based on 3D bioprinting
A 3D printing and glioma technology, applied in the fields of biology and tissue engineering, can solve the problems of 2D culture system, such as lack of cell morphology and function, and indicators that cannot be objectively evaluated
Inactive Publication Date: 2021-04-30
杭州枫霖科技有限公司
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[0022] The implementation of the present invention will be illustrated by specific specific examples below, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification.
[0023] A method for constructing three-dimensional glioma tissue based on biological 3D printing, comprising the following steps:
[0024] (1) Prepare the sponge scaffold material and test its mechanical properties, then use the bio-3D printing technology to add the sponge into the porous plate, go through the freeze-drying process for 24 hours, and then sterilize it with ultraviolet light for 2 hours to obtain the scaffold for constructing glioma. Three-dimensional tissue scaffold;
[0025] (2) Mix the human glioma cells with the culture medium and 3D print them on the sponge scaffold material prepared in step (1), and then culture them in 10% FBS culture medium for 7 days to prepare a three-dimensional glioma Tis...
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Abstract
The invention discloses a method for constructing three-dimensional glioma tissue based on biological 3D printing. The steps are as follows: 1) prepare a sponge scaffold material, and detect its mechanical properties, use biological 3D printing technology to add the sponge into a porous plate, and pass through 24h The freeze-drying process, followed by UV sterilization for 2 hours to obtain a three-dimensional tissue scaffold for constructing glioma; 2) Mix human glioma cells with culture medium and 3D print them on the sponge scaffold material, and then 3D print them on the sponge scaffold material containing 10 Cultured in %FBS medium for 7 days to prepare three-dimensional glioma tissue engineering units; 3) Detect cell viability and glycosaminoglycan secretion in three-dimensional glioma tissue; 4) Use bioequivalence histochemical method to detect 2D and 3D growth status at day 7. The advantage is that the invention effectively promotes the formation of glioma tissue functions, provides useful references for scientific research on brain tissue engineering and clinical treatment of brain cancer, and provides effective methods and technical support for the clinical application of bioprinting technology.
Description
technical field [0001] The invention belongs to the technical field of biology and tissue engineering, and in particular relates to a method based on constructing three-dimensional glioma tissue. Background technique [0002] Drug development requires in vitro and in vivo experiments. Glioma cells have been extensively studied and applied in drug screening and tissue engineering in traditional 2D culture. Many indicators cannot be evaluated objectively, which is still a problem that needs to be overcome. The 3D culture system can better simulate the growth environment of cells in the human body, has a more complete culture system than 2D, and is being widely used in various fields. [0003] The 3D culture system needs to be realized with the help of different biological materials. Biological scaffold materials such as collagen and nanomaterials are widely used in tissue engineering. Cell growth requires certain mechanical strength, and the sponge scaffold material has good...
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IPC IPC(8): C12N5/09B33Y10/00
CPCB33Y10/00C12N5/0693C12N2513/00C12N2533/54
Inventor 崔晓峰程卓高桂芳
Owner 杭州枫霖科技有限公司