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Qualitative and quantitative detection method of paraquat based on dried blood spot sample

A quantitative detection method, dried blood spot technology, applied in the field of qualitative and quantitative detection of paraquat based on dried blood spot samples, can solve the problems of long operation time, complicated operation, large sample size, etc., and achieve short operation time and low invasiveness , the effect of low detection limit

Active Publication Date: 2018-06-12
HEBEI MEDICAL UNIVERSITY
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Problems solved by technology

[0003] Aiming at the technical problems of large sample size, complicated operation, low recovery rate, long operation time and high detection limit of the pretreatment method for paraquat poisoned blood samples in the prior art, the present invention provides a method based on dried blood spots Qualitative and quantitative detection method of paraquat in samples

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  • Qualitative and quantitative detection method of paraquat based on dried blood spot sample
  • Qualitative and quantitative detection method of paraquat based on dried blood spot sample
  • Qualitative and quantitative detection method of paraquat based on dried blood spot sample

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Embodiment 1

[0030] The embodiment of the present invention provides a qualitative and quantitative detection method for paraquat based on dried blood spot samples, specifically as follows:

[0031] 1. Preparation of dried blood spot samples: Take 100 μL of whole blood and drop in On the FTA blood collection card (Whatman Company of the United States), after the blood is completely infiltrated, it is dried in a microwave at 1000-1200W for 4-6 minutes, sealed and packaged, and stored at room temperature.

[0032] 2. Sample pretreatment: use 8mm puncher to prepare Punch holes in the FTA blood collection card, place the blood spot in a 2mL centrifuge tube, add 200μL of mobile phase and 5μl of 4μg / mL internal standard ethyl paraquat, and immediately sonicate for 10 minutes, then centrifuge at 12000r / min for 5min at 4°C , and the supernatant was filtered through a 0.2 μm filter membrane to be tested.

[0033] 3. Preparation of standard curve: Add 5 μL of 0.2 μg / mL–200 μg / mL paraquat stand...

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Abstract

The invention relates to a qualitative and quantitative detection method of paraquat based on a dried blood spot sample. The method is characterized in that a dried blood spot method is adopted for performing preliminary treatment on the sample, and a high performance liquid chromatography / mass spectrometry combined analysis method is adopted for performing detection. The method provided by the invention has the advantages that sampling of a lot of blood is not needed, and the invasion is less; centrifugal separation of blood is not needed, and the operating time is short; the interference caused by blood-borne viruses such as AIDS virus, hepatitis B virus and hepatitis C virus is little, and the detection limit is low; and dried blood spots allow the sample to be stably transported and stored at room temperature, so that re-detection can be conveniently performed at places other than the sampling site or at other times.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a qualitative and quantitative detection method for paraquat based on dried blood spot samples. Background technique [0002] Paraquat is a compound that is highly toxic to both humans and animals. It is the herbicide with the highest mortality rate of acute poisoning in humans. Paraquat poisoning leads to high morbidity and mortality, and there is no specific drug. The mortality rate of oral poisoning can reach 90% %above. With the increase of herbicide poisoning cases such as paraquat, the qualitative and quantitative detection of such poisons is of great significance. Reliable and efficient sample pretreatment method is the first link in the entire detection process, but also the most time-consuming part, accounting for about 80% of the entire detection process. At present, the methods used for pretreatment of blood samples poisoned by paraquat are mainly protei...

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 文迪马春玲杨杨向平于峰
Owner HEBEI MEDICAL UNIVERSITY