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Chicken circular RNA Chr9:10814512|10838667 gene detection primers, methods and applications

A technology for detecting primers and genes, which is applied in the field of genetic engineering, and can solve problems such as drug resistance, food safety bacteria, and enhanced drug resistance of bacterial strains

Active Publication Date: 2021-11-30
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the main method to control Salmonella infection is the use of vaccines and antibiotics, but this cannot fundamentally solve the problem, and the extensive use of antibiotics will cause food safety problems and bacterial drug resistance, and lead to enhanced drug resistance of bacterial strains

Method used

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  • Chicken circular RNA Chr9:10814512|10838667 gene detection primers, methods and applications
  • Chicken circular RNA Chr9:10814512|10838667 gene detection primers, methods and applications
  • Chicken circular RNA Chr9:10814512|10838667 gene detection primers, methods and applications

Examples

Experimental program
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Embodiment 1

[0032] Example 1 Establishment of detection method for chicken circular RNA Chr9:10814512|10838667 gene

[0033] Select individuals negative for Salmonella Enteritidis, and inoculate Salmonella Enteritidis by oral method, the inoculation volume is 10 7 -10 9 cfu / only, on the 7th day after inoculation, the contents of the cecum were collected, and the bacterial content was determined by plate counting method. Take cecal tissue samples, extract RNA, and process the extracted RNA with RNase R to digest linear RNA, and perform reverse transcription to generate cDNA.

[0034] According to the gene sequence of chicken circular RNA Chr9:10814512|10838667, specific circular and linear primers (Table 1-2) were designed for amplification.

[0035] Table 1: Chicken circular RNA Chr9:10814512|10838667 circular primer sequence

[0036]

[0037] Table 2: Chicken circular RNA Chr9:10814512|10838667 linear primer sequence

[0038]

[0039] Quantitative PCR amplification was performe...

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Abstract

The invention discloses a detection primer, method and application of chicken circular RNA Chr9:10814512|10838667 gene. The detection primers include chicken circular RNA Chr9:10814512|10838667 circular upstream primer and chicken circular RNA Chr9:10814512|10838667 circular downstream primer, the nucleotide sequences of which are shown in SEQ ID NO.1 and SEQ ID NO.2 respectively . The above primers and method can be used to accurately detect the expression of the Salmonella Enteritidis infection resistance-related circular RNA, and provide a theoretical and scientific basis for the screening of chickens resistant to Salmonella Enteritidis infection and disease-resistant breeding.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a detection primer, method and application of chicken circular RNA Chr9:10814512|10838667 gene. Background technique [0002] Salmonella enteritidis (SE) is a common zoonotic pathogen. Because it has no host specificity, it can not only cause diseases such as acute gastroenteritis in poultry and even cause death, causing huge economic losses to the poultry industry, but also seriously endangering humans. Health, causing food poisoning, is one of the important pathogenic bacteria that affect people's physical and mental health. Relevant reports pointed out that during 2004, 19,000 people per 1 million people in the United States were hospitalized due to Salmonella infection, and as many as 300 people died due to Salmonella infection; Disease outbreak. Poultry and related processed products are the main source of infection of Salmonella Enteritidis, and th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12N15/11C12R1/42
CPCC12Q1/6888C12Q2600/158C12Q2600/178
Inventor 李显耀郑林娜刘丽英王园美
Owner SHANDONG AGRICULTURAL UNIVERSITY
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