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Medicinal application of collagen protein peptide, cucurbitacin oral micelle, and preparation method thereof

A collagen peptide and cucurbitacin technology, applied in the field of medicine, can solve the problems of complicated preparation methods and high cost, and achieve the effects of improving bioavailability, low cost and improving membrane permeability

Active Publication Date: 2018-06-29
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the above-mentioned technical problems, the present invention provides the pharmaceutical application of collagen peptide, cucurbitacin oral micelles and its preparation method. A low-cost, high-efficiency preparation method of collagen peptides that can promote the oral absorption of drugs. Another object of the present invention is the application of positively charged collagen peptides in oral administration, and provides collagen peptides containing Cucurbitacin Oral Micelles of Protein Peptides Enhance the Oral Bioavailability of Cucurbitacin

Method used

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  • Medicinal application of collagen protein peptide, cucurbitacin oral micelle, and preparation method thereof
  • Medicinal application of collagen protein peptide, cucurbitacin oral micelle, and preparation method thereof
  • Medicinal application of collagen protein peptide, cucurbitacin oral micelle, and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Preparation of positively charged collagen peptides: Weigh 5 g of D152 cation exchange resin and place it in a conical flask, add 10 mL of collagen peptide aqueous solution with a mass concentration of 0.1 g / mL, wherein the molecular weight of the collagen peptide is 3000 Da, After oscillating and adsorbing in a shaker at 40°C for 3 hours, collect the adsorbed resin, elute with 10 mL of 0.2 mol / L sodium chloride aqueous solution for 2 hours, filter, and discard the eluent; mol / L sodium chloride aqueous solution repeats the same steps for elution 3 times, collects the eluate, and combines the obtained eluents to obtain the total solution, concentrates to remove salt, dialyzes with a dialysis bag, and freeze-dries to obtain a positively charged collagen peptide , its appearance is white soft powder with obvious crystal luster.

Embodiment 2

[0048] Analysis of the amino acid composition of collagen peptides: Weigh 0.1 g of commercially available collagen peptides and the collagen peptides prepared in Example 1, add 10 ml of HCl with a concentration of 6 mol / L, oscillate and mix well, seal and place in an oven at 110°C Hydrolyze for 24 hours, filter the hydrolyzate, take 500μl filtrate, vacuum dry at 55°C, add 0.02mol / L HCl to the dried sample to dissolve and dilute, mix the standard solution with amino acid for ninhydrin colorimetry, determine the degree of sample dilution, use 0.22 μm microporous membrane filtration, automatic amino acid analyzer for analysis, the composition and content changes of different amino acid types in collagen peptides before and after separation are shown in Table 1;

[0049] Table 1 Amino acid composition analysis of 3000Da commercially available collagen peptides and positively charged collagen peptides

[0050]

[0051]

[0052] The amount of acidic amino acid and basic amino ...

Embodiment 3

[0054] Preparation of collagen peptide-total cucurbitacin mixed nanomicelles:

[0055] 1) Prepare 5 mg / mL total cucurbitacin methanol solution (based on the concentration of cucurbitacin B, the content of cucurbitacin B is 65%), take 2 mL of the above solution, add 48 mg lecithin, and stir at 40 ° C for 45 min;

[0056] 2) Weigh 71 mg of caprylic capric acid macrogol glyceride and 29 mg of polyethylene glycol 15-hydroxystearate, add them to the mixed solution obtained in step 1), and continue stirring for 45 min at the same temperature;

[0057] 3) Evaporate the solution obtained in step 2) to remove methanol, blow with nitrogen to completely remove the organic solvent, and obtain a uniformly distributed transparent drug-containing lipid film;

[0058] 4) Add 17 mL of purified water to dissolve the lipid film obtained in step 3), and after ultrasonic hydration at 40°C for 1 hour, centrifuge at 10,000 rpm for 20 minutes;

[0059] 5) Take the supernatant obtained in step 4), ad...

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Abstract

The invention belongs to the technical field of medicines and especially relates to a medicinal application of collagen protein peptide, a cucurbitacin oral micelle, and a preparation method thereof.The application is that the collagen protein peptide is used as a medicine transmembrane tool in preparation of the oral medicine preparation, wherein the collagen protein peptide carries positive charges. The invention provides the cucurbitacin oral micelle containing the collagen protein peptide, which comprises, by weight, 8-15 parts of cucurbitacin, 30-70 parts of lecithin, 50-75 parts of octanoic decanoic polyethylene glycol glyceride, 25-50 parts of polyethylene glycol 15-hydroxystearate and 80-100 parts of the collagen protein peptide carrying the positive charges. The collagen proteinpeptide, cooperated with other carrier materials, can improve membrane-permeability of the medicine and reduce excretion of the medicine, so that the collagen protein peptide can be used as the medicine transmembrane tool. For solving problems about oral bioavailability of the cucurbitacin, the invention provides the technology of collagen protein peptide coating nano-micelle, wherein by means ofthe characters of the collagen protein peptide, the problem of low bioavailability of medicines, such as the cucurbitacin, is solved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the pharmaceutical application of collagen peptide, cucurbitacin oral micelles and a preparation method thereof. Background technique [0002] The clinical application of drugs is mostly oral administration, and the efficacy of the drug mainly depends on the absorption of the effective components in the intestinal tract. Biopharmaceutical properties such as solubility, stability, membrane permeability and first-pass action of drugs are the main factors affecting the absorption of drugs through the gastrointestinal tract after oral administration. Many active ingredients have low solubility and poor permeability, and due to reasons such as transporter efflux and intestinal metabolism, oral bioavailability is very low, which greatly restricts the clinical efficacy of drugs. The research reports on improving the oral bioavailability of drugs by using new preparation te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/107A61K47/42A61K47/14A61K31/575A61P1/16A61P35/00
CPCA61K9/0053A61K9/1075A61K31/575A61K47/14A61K47/42
Inventor 唐岚傅璐璐王建张振海杨燕单伟光
Owner ZHEJIANG UNIV OF TECH
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