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Gene for improving cadmium accumulation and tolerance of plant, and application thereof

A plant and tolerance technology, applied in the field of bioengineering, can solve the problems of concealed irreversibility of pollution, pollution of surface water and groundwater, long duration, etc.

Inactive Publication Date: 2018-07-06
HEFEI UNIV OF TECH
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Problems solved by technology

This kind of pollution has a wide range, long duration, hidden and irreversible pollution, and cannot be degraded by microorganisms like organic matter, which eventually leads to soil degradation, decline in crop yield and quality, and pollutes surface water and groundwater through runoff and leaching.

Method used

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  • Gene for improving cadmium accumulation and tolerance of plant, and application thereof
  • Gene for improving cadmium accumulation and tolerance of plant, and application thereof
  • Gene for improving cadmium accumulation and tolerance of plant, and application thereof

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Embodiment Construction

[0023] Below in conjunction with embodiment, the present invention will be further described.

[0024] 1. MYB4 gene overexpression line MYB4-OE1, MYB4-OE2 the acquisition

[0025] In order to verify the function of this gene in the regulation of plant heavy metal cadmium stress, we constructed MYB4 Gene overexpression vector. First, the target fragment is amplified. The wild-type Arabidopsis was normally cultured on ½MS medium for two weeks, the total RNA of the plant was extracted, reverse-transcribed to synthesize cDNA, and the synthesized cDNA was used as a template for PCR to amplify a sufficient amount of the target product. Using the PCR product as a template, a second amplification was performed to introduce restriction sites. The PCR product and vector were digested and recovered. Then, the recovered and purified target DNA fragment and the vector were ligated overnight with T4 DNA ligase. The above ligation solution was transferred into DH5a, and positive cl...

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Abstract

The invention relates to a gene for improving the cadmium accumulation and the tolerance of a plant, and application thereof. The gene for improving the cadmium accumulation and the tolerance of the plant has a sequence as shown in a sequence table SEQ ID No:1, and is applied to repairing cadmium-polluted soil. The gene for improving the cadmium tolerance of the plant is transferred into the plantso as to be overexpressed in the plant, and the plant is cadmium-tolerant. A research result shows that under the condition of cadmium treatment, through overexpressing an MYB4 gene, the gene can bebinded with mannose, and the increased expression of related genes such as GSH1, GSH2, PCS1 and PCS2 is promoted, so that the contents of GSH and PC are increased; an external cadmium ion is stored invacuole, and the cadmium tolerance is expressed.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to using genes to improve plant tolerance to cadmium and its application in repairing cadmium pollution in soil. Background technique [0002] With the continuous development of industry and agriculture, heavy metal pollution in soil is becoming more and more severe. Heavy metals in polluted soil mainly include Cd, Pb, Hg, AS, and Zn, Cu, etc. with certain toxicity. This kind of pollution has a wide range, long duration, hidden and irreversible pollution, and cannot be degraded by microorganisms like organic matter, which eventually leads to soil degradation, decline in crop yield and quality, and pollutes surface water and groundwater through runoff and leaching. Moreover, it will also enter the human body through the food chain and be absorbed by the human body, endangering human health. Heavy metals entering the human body will continue to accumulate and conc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/82A01H5/00A01H6/46A01H6/20A01H6/82A01H6/34
CPCC07K14/415C12N15/8271
Inventor 曹树青欧阳剑黄莹严星星樊婷婷刘永胜陈峰张乘
Owner HEFEI UNIV OF TECH
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