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Method for reducing raratrioza sinica and improving production property of wolfberry by biological preventing and controlling

A technology of wolfberry psyllid and production performance, which is applied in the field of biological control, can solve the problems of increasing the dosage of pesticides, increasing pesticide residues, and affecting the quality of wolfberry, so as to improve the survival rate, reduce the wolfberry psyllid, and improve the production performance of wolfberry Effect

Inactive Publication Date: 2018-07-13
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although pesticides can control the harm of psyllids, long-term use will cause psyllids to develop drug resistance, resulting in increased dosage of pesticides and increased pesticide residues in wolfberry products, which will affect the quality of wolfberries

Method used

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  • Method for reducing raratrioza sinica and improving production property of wolfberry by biological preventing and controlling
  • Method for reducing raratrioza sinica and improving production property of wolfberry by biological preventing and controlling
  • Method for reducing raratrioza sinica and improving production property of wolfberry by biological preventing and controlling

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] 1) High-density culture of chitinase-producing Sb, the starting density of Sb is 2.75×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 11g, soybean powder 20g, shrimp powder 1g, the balance was water, adjusted to pH=6.5, sterilized at 121°C for 20min), the inoculum size was 2%, 36 Cultivate with shaking at ℃ for 22 hours, and the density of the bacterial solution is 3.30×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0058] 2) Add corn flour and water and stir to make corn milk, the concentration of corn milk is 50%, add raw amylase composed of α-amylase (EC.3.2.1.1) and glucoamylase (EC.3.2.1.3) (the enzyme dosage is respectively 5U / L and 500U / L), cultured with shaking at 37°C for 24hrs, the starch in the cornflour granules was partially hydrolyzed to form holes, and dried at 120°C after centrifugation to become microporous cornflour.

[0059] 3) Add microporous corn flour to the Sb bacterial solution to a concentration...

Embodiment 2

[0065] 1) High-density culture of chitinase-producing Sb, the starting density of Sb is 3.14×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 17g, soybean powder 30g, shrimp powder 3g, the balance was water, adjusted to pH=7.5, sterilized at 121°C for 20min), the inoculum size was 4%, 38 Cultivate with shaking at ℃ for 26 hours, and the density of the bacterial solution is 6.58×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0066] 2) Add corn flour and water and stir to make corn milk, the concentration of corn milk is 50%, add raw amylase composed of α-amylase (EC.3.2.1.1) and glucoamylase (EC.3.2.1.3) (the enzyme dosage is respectively 5U / L and 500U / L), cultured with shaking at 37°C for 24hrs, the starch in the cornflour granules was partially hydrolyzed to form holes, and dried at 120°C after centrifugation to become microporous cornflour.

[0067] 3) Add microporous corn flour to the concentration of 5% (W / V) in the Sb bact...

Embodiment 3

[0073] 1) High-density culture of chitinase-producing Sb, the starting density of Sb is 2.18×10 6 Individuals / mL, inoculated into liquid culture medium (based on 1 L, glucose 14g, soybean powder 25g, shrimp powder 2g, the balance was water, adjusted to pH=7.0, sterilized at 121°C for 20min), the inoculum size was 3%, 37 Cultivate with shaking at ℃ for 24 hours, and the density of the bacterial solution is 6.32×10 9 cfu / mL to obtain a high-density Sb bacterial solution.

[0074] 2) Add corn flour and water and stir to make corn milk, the concentration of corn milk is 50%, add raw amylase composed of α-amylase (EC.3.2.1.1) and glucoamylase (EC.3.2.1.3) (the enzyme dosage is respectively 5U / L and 500U / L), cultured with shaking at 37°C for 24hrs, the starch in the cornflour granules was partially hydrolyzed to form holes, and dried at 120°C after centrifugation to become microporous cornflour.

[0075] 3) Add microporous corn flour to the concentration of 5% (W / V) in the Sb bact...

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Abstract

The invention discloses a method for reducing praratrioza sinica and improving production property of wolfberry by biological preventing and controlling. Bacillus subtilis of high-yield chitinase bacillus subtilis (CICC 10339) (short for Sb) is prepared into a bacterial agent, and the bacterial agent is combined with a biological fertilizer to use, so as to reduce the praratrioza sinica and improve the production property of wolfberry, thereby providing a green biological preventing and controlling strategy. The method comprises the following steps of culturing the Sb, and preparing an Sb bacterial liquid with density of 10<9> to 10<10>cfu / mL; hydrolyzing corn flour by raw amylase, so as to prepare microporous corn flour; embedding the Sb into micropores of the microporous corn flour, so as to prepare an Sb bacterial liquid of microporous corn flour; preparing the Sb bacterial liquid of microporous corn flour into an Sb bacterial agent; using the Sb bacterial liquid as a fermenting agent to prepare an Sb biological fertilizer; spraying the Sb bacterial agent to the surface of a wolfberry plant, and applying the Sb biological fertilizer to the root of the wolfberry plant, thereby effectively reducing the praratrioza sinica.

Description

technical field [0001] The invention relates to the field of biological control, in particular to a method for reducing wolfberry psyllids and improving production performance through biological control. Background technique [0002] Pests and diseases occur in various growth stages of wolfberry, among which psyllid is one of the main pests and diseases. Lycium barbarum psyllids have 3-4 generations per year, overwinter as adults on soil clods, tree trunks, litter, bark, or wall cracks, and start to move when wolfberry germinates in the next spring, laying eggs on the back or surface of leaves, yellow , dense as hair, commonly known as jaundice, blooms from June to July, adults and nymphs insert their mouthparts into the leaf tissue on the back of the leaf, suck the juice, and excrete a large amount of white feces, resulting in yellow leaves and thin branches, and tree vigor is weak. Berry development was inhibited and quality decreased. Nymphs are more dangerous than adul...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P7/04A01P21/00C05G3/02C12N1/20C12N11/04C12R1/125C05G3/60
CPCA01N63/10C05D1/00C05G3/60C12N1/20C12N11/04C05F1/005C05F5/002C05F11/00C05F11/08
Inventor 阮晖胡超凡金迅邱亦亦丁丽娜
Owner ZHEJIANG UNIV
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