Construction method for immune model of cryptocaryon irritans to locally infect gill tissues of epinephelus coioides

A technology to stimulate Cryptocaryonia and grouper, applied in fish farming, application, climate change adaptation, etc., can solve problems such as differences in immune response that cannot be ruled out

Active Publication Date: 2018-07-20
SOUTH CHINA AGRI UNIV
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Problems solved by technology

However, this method cannot rule out differences in the immune response of the same fish under normal conditions in the tail and skin other than the tail

Method used

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specific Embodiment approach

[0026] A method for constructing an immune model that stimulates the local infection of Cryptocaryon spp. clotted grouper gill tissue, comprising the following steps:

[0027] 1. Stimulate the collection and concentration calculation of live Cryptocaryon larvae

[0028] Cryptocaryon stimuli were subcultured and collected using a special subculture system for Cryptocaryon stimuli, and the subculture host was Pompano ovata. Collect well-developed Cryptocaryon irritant cysts, place them in a sterilized clean beaker and incubate them with high-pressure sterilized natural seawater with appropriate amount of penicillin and streptomycin added; collect irritant cryptocysts within 2 hours of hatching. Take 50 μL of nuclei larva fluid after mixing thoroughly, add 10 μL of formaldehyde to kill the larvae, count under a microscope, and calculate the concentration of larvae in the fluid.

[0029] 2. Grouping and domestication of grouper

[0030] The fish used in the experiment is about 2...

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Abstract

The invention discloses a method for immunization by using cryptocaryon irritans to locally infect the unilateral gill of epinephelus coioides for the first time, the optimized infection concentrationand infection duration are used to construct an immune model of the cryptocaryon irritans to locally infect gill tissues of the epinephelus coioides for the first time, the negative effects caused bycongenital difference in the immune response process of fish local gill tissue mucous membranes are completely eliminated, and an ideal immune model is provided for studying the local mucous membraneimmune response rule. A construction method for the immune model mainly comprises five steps of (1), collecting cryptocaryon irritans live larvae, and conducting concentration calculation; (2), conducting grouping and domestication on the epinephelus coioides; (3), conducting local gill tissue immunization; (4), measuring infection intensity; (5) treating a fish body after local infection.

Description

technical field [0001] The invention belongs to the field of fish immunity, in particular to the direction of fish mucosal immunity, and specifically relates to a method for constructing an immune model for inducing local mucosal immunity of gill tissue of oblique-banded grouper. Background technique [0002] Cryptocaryon irritans (Cryptocaryon irritans) is a protociliate that can infect almost all marine bony fishes and cause severe fatal diseases - Cryptocaryon irritans or white spot disease (Colorni A and BurgessP., 1997) . The life cycle of Cryptocaryon stimuli includes four stages: trophozoite, cyst precursor, cyst and larvae, among which only the trophozoite is an obligate parasitic life and feeds on host body fluids, tissue fragments and intact cells. In recent years, due to the overloaded development of marine aquaculture in my country and the deterioration of the aquaculture environment, Cryptocaryoniasis has become a large-scale, frequent outbreak of severe infect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 李言伟但学明莫泽权
Owner SOUTH CHINA AGRI UNIV
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