A Depth Imaging Super-resolution Microscopic Imaging System

Abstract

The invention discloses a depth imaging super-resolution microscopic imaging system. The depth imaging super-resolution microscopic imaging system includes a first laser, a first lens, a first dichroscope, a second dichroscope, a second laser, a sixth lens, a phase modulation assembly, a third dichroscope, a deformation reflector, an X axis scanning vibrating mirror, a phase compensation assembly,a Y axis scanning vibrating mirror, a vertical optical path refraction matching unit, a second quarter wave-plate, a microscopic object lens, a sample table, a horizontal optical path refraction matching unit, a first optical filter, a wavefront sensor, a second optical filter, a tenth lens and an imaging device. By means of the two-photon effect, by compensating the fluorescent wavefront phase change through the wavefront sensor and the deformation reflector, by combining with movement of the sample table in the Z axis direction, the depth imaging super-resolution microscopic imaging systemcan realize three-dimensional depth imaging while correcting the aberration caused by the samples, thus being relatively higher in the imaging quality.

Description

technical field [0001] The invention relates to the field of optical microscopic imaging, in particular to a depth imaging super-resolution microscopic imaging system. Background technique [0002] In a conventional optical microscopic imaging system, due to the diffraction effect of optical components, the light spot formed on the sample after the parallel incident illumination light is focused by the microscopic objective lens is not an ideal spot, but a spot with a certain size. Diffraction spot, according to Abbe's diffraction limit formula, the diameter of the smallest spot that can be focused by visible light is about 200nm. In 1994, German scientist S.W.Hell first proposed STED super-resolution microscopy imaging technology, which surpassed the diffraction limit and achieved a spatial resolution of 30 nanometers in 2006. This outstanding work won him the Nobel Prize in Chemistry in 2014. prize. [0003] The basic idea of ​​STED super-resolution is to use the stimula...

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Problems solved by technology

[0006] The present invention aims at the aberration problem of sample depth direction scanning existing when the existing super-resolution microscope system realizes three-dimensional imaging of the biological sample structure, and proposes a depth imaging super-resolution microscope system

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