Human liver cancer marker and application thereof

A marker and liver cancer technology, applied in the field of biomedicine, can solve the problems of less cancer research, no report on the role of liver cancer, and decreased expression of GPT

Inactive Publication Date: 2018-07-24
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The research results of our laboratory show that in human liver cancer tissues, the expression of GPT is significantly decreased. At present, the relevant reports on the function of GPT at h

Method used

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  • Human liver cancer marker and application thereof
  • Human liver cancer marker and application thereof
  • Human liver cancer marker and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: Bioinformatics analysis: using the GEO tumor public database to retrieve the liver cancer expression profile data in the database, the research team collected and sorted it out in the early stage and then applied to search the liver cancer expression profile data set in the GEO tumor gene chip public data set and the TCGA database The HCC cohort included 10 datasets with matched cancer and paracancerous samples (see Table 1), with a total of 1255 paracancerous and 1476 liver cancer tissues. Download the liver cancer expression profile sequencing data queue, analyze the expression of GPT gene in liver cancer and para-cancerous tissues in different research cohorts, analysis method: use BRB-array tools gene expression analysis tool, import the downloaded liver cancer expression profile file, select mRNA as the data type , export the expression data of GPT, and carry out the matching of clinical data, and carry out the difference analysis, the result indicates th...

Embodiment 2

[0041]Example 2: Using the TCGA tumor public database to download the expression profile sequencing data of liver cancer. Analyze the expression of GPT gene in liver cancer and para-cancerous tissues, analysis method: use BRB-array tools gene expression analysis tool, import the downloaded liver cancer expression profile file, select mRNA as the data type, export the expression data of GPT, and carry out clinical data analysis The results of GPT showed significantly low expression in liver cancer; then KM survival analysis results showed that the overall survival rate of patients with low GPT expression was significantly lower than that of patients with low GPT expression, and the patients with low GPT expression The progression-free survival rate was significantly lower than that of patients with low expression of GPT.

Embodiment 3

[0042] Example 3: Analysis of the expression of GPT protein in liver cancer and adjacent normal tissues by immunohistochemistry

[0043] Tissue chip: using the pan-tumor tissue chip constructed by the research group, the source of the tissue sample is the First Affiliated Hospital of Zhengzhou University, where the tumor type includes (), and the clinical information includes gender, age, TNM, tumor size,

[0044] Staging, grading, follow-up information, etc. The diameter of the chip sample points is 1.5mm, and the fixation method is formalin; at the same time, a commercial tissue chip (Shanghai Xinchao Biotechnology Co., Ltd.) is used. Including gender, age, TNM, tumor size,

[0045] Staging, grading, follow-up information, etc. The diameter of the chip sample points is 1.5mm, and the fixation method is formalin fixation. The main operation steps of immunohistochemistry: put the tissue chip into the oven, adjust the temperature to 63 degrees, and bake the wax for one hour;...

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Abstract

The invention relates to the technical field of biomedicines, discloses a human liver cancer marker and application thereof, and particularly relates to application of a GPT gene to diagnosis and treatment of hepatocellular carcinoma. By a gene chip data analysis and immunohistochemistry method, that the GPT gene is differentially expressed in a hepatocellular carcinoma tissue and a normal tissueis proved, and that expression of the GPT gene is closely related to clinical prognosis of a patient and the GPT gene can be used as an index for early diagnosing the hepatocellular carcinoma is confirmed. Through study on the expression of the GPT gene in liver cancer and a relationship between the GPT gene and the clinical prognosis, a new marker is provided for early diagnosis and prognostic judgment of the liver cancer so as to improve the early diagnosis rate of a liver cancer patient and improve the clinical prognosis of the patient.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and relates to a human liver cancer marker and its application, in particular to the application of GPT gene in the diagnosis and treatment of hepatocellular carcinoma. Background technique [0002] Hepatocellular carcinoma is one of the malignant tumors with extremely high morbidity and mortality in the world, and the mortality rate of liver cancer in my country ranks second among malignant tumors. Due to the insidious onset and early metastasis of hepatocellular carcinoma, most patients have missed the opportunity of surgical resection at the time of diagnosis, and because liver cancer is not sensitive to chemotherapy and radiotherapy, even if radical resection of liver cancer is performed, there will still be 60-70% chance Patients with liver cancer relapse within 5 years, making the treatment of liver cancer still face great challenges. At present, the main diagnostic methods for liver c...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12Q1/6837G01N33/574
CPCC12Q1/6837C12Q1/6886C12Q2600/118C12Q2600/158G01N33/57438
Inventor 孙冉冉
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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